Analysis of 16S rDNA reveals bacterial shift during in vitro fermentation of fermentable carbohydrate using piglet faeces as inoculum

Zhu Wei-Yun, B.A. Williams, S.R. Konstantinov, S. Tamminga, W.M. de Vos, A.D.L. Akkermans

Research output: Contribution to journalArticleAcademicpeer-review

42 Citations (Scopus)

Abstract

In vitro fermentation of sugar beet pulp (SBP) was carried out to determine which bacterial species would be enriched by use of this carbohydrate source. Faeces from four weaning piglets as a source of inoculum was also compared. The microbial diversity of the prominent bacteria before and after this in vitro fermentation was analysed using denaturing gradient gel electrophoresis (DGGE) of PCR amplicons of 16S rDNA. Before fermentation, the DGGE profiles showed differences between cultures inoculated with faeces from different piglets, though some bands were common to all piglets. After fermentation of SBP, three dominant bands appeared, of which two bands appeared in all samples and one for both replicates of one piglet. Sequences of the corresponding 16S rDNA of two bands showed 92% similarity to Eubacterium eligens and 96% similarity to Lachnospira pectinoschiza, and that of the third band 95% to L. pectinoschiza. (C) 2003 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)175-180
JournalAnaerobe
Volume9
Issue number4
DOIs
Publication statusPublished - 2003

Fingerprint

Ribosomal DNA
Feces
Fermentation
Carbohydrates
Denaturing Gradient Gel Electrophoresis
Beta vulgaris
Eubacterium
Weaning
Bacteria
Polymerase Chain Reaction
In Vitro Techniques

Keywords

  • gradient gel-electrophoresis
  • ribosomal-rna
  • grassland soils
  • gas-production
  • sp-nov
  • intestine
  • pigs
  • dna
  • communities
  • microbiota

Cite this

Zhu Wei-Yun ; Williams, B.A. ; Konstantinov, S.R. ; Tamminga, S. ; de Vos, W.M. ; Akkermans, A.D.L. / Analysis of 16S rDNA reveals bacterial shift during in vitro fermentation of fermentable carbohydrate using piglet faeces as inoculum. In: Anaerobe. 2003 ; Vol. 9, No. 4. pp. 175-180.
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abstract = "In vitro fermentation of sugar beet pulp (SBP) was carried out to determine which bacterial species would be enriched by use of this carbohydrate source. Faeces from four weaning piglets as a source of inoculum was also compared. The microbial diversity of the prominent bacteria before and after this in vitro fermentation was analysed using denaturing gradient gel electrophoresis (DGGE) of PCR amplicons of 16S rDNA. Before fermentation, the DGGE profiles showed differences between cultures inoculated with faeces from different piglets, though some bands were common to all piglets. After fermentation of SBP, three dominant bands appeared, of which two bands appeared in all samples and one for both replicates of one piglet. Sequences of the corresponding 16S rDNA of two bands showed 92{\%} similarity to Eubacterium eligens and 96{\%} similarity to Lachnospira pectinoschiza, and that of the third band 95{\%} to L. pectinoschiza. (C) 2003 Elsevier Ltd. All rights reserved.",
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Analysis of 16S rDNA reveals bacterial shift during in vitro fermentation of fermentable carbohydrate using piglet faeces as inoculum. / Zhu Wei-Yun; Williams, B.A.; Konstantinov, S.R.; Tamminga, S.; de Vos, W.M.; Akkermans, A.D.L.

In: Anaerobe, Vol. 9, No. 4, 2003, p. 175-180.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Zhu Wei-Yun, null

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AB - In vitro fermentation of sugar beet pulp (SBP) was carried out to determine which bacterial species would be enriched by use of this carbohydrate source. Faeces from four weaning piglets as a source of inoculum was also compared. The microbial diversity of the prominent bacteria before and after this in vitro fermentation was analysed using denaturing gradient gel electrophoresis (DGGE) of PCR amplicons of 16S rDNA. Before fermentation, the DGGE profiles showed differences between cultures inoculated with faeces from different piglets, though some bands were common to all piglets. After fermentation of SBP, three dominant bands appeared, of which two bands appeared in all samples and one for both replicates of one piglet. Sequences of the corresponding 16S rDNA of two bands showed 92% similarity to Eubacterium eligens and 96% similarity to Lachnospira pectinoschiza, and that of the third band 95% to L. pectinoschiza. (C) 2003 Elsevier Ltd. All rights reserved.

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