Anaerobic Degradation of N-ϵ-Carboxymethyllysine, a Major Glycation End-Product, by Human Intestinal Bacteria

Thi Phuong Nam Bui, Antonio Dario Troise, Vincenzo Fogliano*, Willem M. De Vos

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

Modifications of lysine contribute to the amount of dietary advanced glycation end-products reaching the colon. However, little is known about the ability of intestinal bacteria to metabolize dietary N-ϵ-carboxymethyllysine (CML). Successive transfers of fecal microbiota in growth media containing CML were used to identify and isolate species able to metabolize CML under anaerobic conditions. From our study, only donors exposed to processed foods degraded CML, and anaerobic bacteria enrichments from two of them used 77 and 100% of CML. Oscillibacter and Cloacibacillus evryensis increased in the two donors after the second transfer, highlighting that the bacteria from these taxa could be candidates for anaerobic CML degradation. A tentative identification of CML metabolites produced by a pure culture of Cloacibacillus evryensis was performed by mass spectrometry: carboxymethylated biogenic amines and carboxylic acids were identified as CML degradation products. The study confirmed the ability of intestinal bacteria to metabolize CML under anoxic conditions.

Original languageEnglish
Pages (from-to)6594-6602
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume67
Issue number23
DOIs
Publication statusPublished - 12 Jun 2019

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Keywords

  • dietary advanced glycation end-products
  • intestinal metabolism
  • Maillard reaction
  • microbiota
  • N-ϵ-carboxymethyllysine

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