Anaerobic Degradation of N-ϵ-Carboxymethyllysine, a Major Glycation End-Product, by Human Intestinal Bacteria

Thi Phuong Nam Bui, Antonio Dario Troise, Vincenzo Fogliano, Willem M. De Vos

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Modifications of lysine contribute to the amount of dietary advanced glycation end-products reaching the colon. However, little is known about the ability of intestinal bacteria to metabolize dietary N-ϵ-carboxymethyllysine (CML). Successive transfers of fecal microbiota in growth media containing CML were used to identify and isolate species able to metabolize CML under anaerobic conditions. From our study, only donors exposed to processed foods degraded CML, and anaerobic bacteria enrichments from two of them used 77 and 100% of CML. Oscillibacter and Cloacibacillus evryensis increased in the two donors after the second transfer, highlighting that the bacteria from these taxa could be candidates for anaerobic CML degradation. A tentative identification of CML metabolites produced by a pure culture of Cloacibacillus evryensis was performed by mass spectrometry: carboxymethylated biogenic amines and carboxylic acids were identified as CML degradation products. The study confirmed the ability of intestinal bacteria to metabolize CML under anoxic conditions.

LanguageEnglish
Pages6594-6602
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume67
Issue number23
DOIs
Publication statusPublished - 12 Jun 2019

Fingerprint

glycation
intestinal microorganisms
anaerobic conditions
Bacteria
Degradation
degradation
bacteria
biogenic amines
processed foods
carboxylic acids
colon
lysine
culture media
mass spectrometry
metabolites
Processed foods
N(6)-carboxymethyllysine
Advanced Glycosylation End Products
Biogenic Amines
Anaerobic Bacteria

Keywords

  • dietary advanced glycation end-products
  • intestinal metabolism
  • Maillard reaction
  • microbiota
  • N-ϵ-carboxymethyllysine

Cite this

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title = "Anaerobic Degradation of N-ϵ-Carboxymethyllysine, a Major Glycation End-Product, by Human Intestinal Bacteria",
abstract = "Modifications of lysine contribute to the amount of dietary advanced glycation end-products reaching the colon. However, little is known about the ability of intestinal bacteria to metabolize dietary N-ϵ-carboxymethyllysine (CML). Successive transfers of fecal microbiota in growth media containing CML were used to identify and isolate species able to metabolize CML under anaerobic conditions. From our study, only donors exposed to processed foods degraded CML, and anaerobic bacteria enrichments from two of them used 77 and 100{\%} of CML. Oscillibacter and Cloacibacillus evryensis increased in the two donors after the second transfer, highlighting that the bacteria from these taxa could be candidates for anaerobic CML degradation. A tentative identification of CML metabolites produced by a pure culture of Cloacibacillus evryensis was performed by mass spectrometry: carboxymethylated biogenic amines and carboxylic acids were identified as CML degradation products. The study confirmed the ability of intestinal bacteria to metabolize CML under anoxic conditions.",
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Anaerobic Degradation of N-ϵ-Carboxymethyllysine, a Major Glycation End-Product, by Human Intestinal Bacteria. / Bui, Thi Phuong Nam; Troise, Antonio Dario; Fogliano, Vincenzo; De Vos, Willem M.

In: Journal of Agricultural and Food Chemistry, Vol. 67, No. 23, 12.06.2019, p. 6594-6602.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Anaerobic Degradation of N-ϵ-Carboxymethyllysine, a Major Glycation End-Product, by Human Intestinal Bacteria

AU - Bui, Thi Phuong Nam

AU - Troise, Antonio Dario

AU - Fogliano, Vincenzo

AU - De Vos, Willem M.

PY - 2019/6/12

Y1 - 2019/6/12

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AB - Modifications of lysine contribute to the amount of dietary advanced glycation end-products reaching the colon. However, little is known about the ability of intestinal bacteria to metabolize dietary N-ϵ-carboxymethyllysine (CML). Successive transfers of fecal microbiota in growth media containing CML were used to identify and isolate species able to metabolize CML under anaerobic conditions. From our study, only donors exposed to processed foods degraded CML, and anaerobic bacteria enrichments from two of them used 77 and 100% of CML. Oscillibacter and Cloacibacillus evryensis increased in the two donors after the second transfer, highlighting that the bacteria from these taxa could be candidates for anaerobic CML degradation. A tentative identification of CML metabolites produced by a pure culture of Cloacibacillus evryensis was performed by mass spectrometry: carboxymethylated biogenic amines and carboxylic acids were identified as CML degradation products. The study confirmed the ability of intestinal bacteria to metabolize CML under anoxic conditions.

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