An updated conventional- and a novel GM potato late blight R gene differential set for virulence monitoring of Phytophthora infestans

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Abstract

Late blight is an important disease in potato that is caused by the oomycete Phytophthora infestans. In the past, Solanum demissum late blight resistance (R) genes were introgressed into cultivated potato (Solanum tuberosum). Eleven of these resistant plants were selected to characterize the virulence spectrum of individual P. infestans isolates and to monitor the dynamics of virulence in P. infestans populations. These plants are referred to as the Mastenbroek and Black differential sets. It has long been assumed that each differential plant contained one single R gene. In the current study and previous studies, however, most Mastenbroek differential plants were shown to harbor multiple R gene(s), which blurs virulence typing of late blight isolates. In order to acquire more accurate virulence profiles, we extended the Mastenbroek differential set with Solanum spp. plants harboring reduced R gene complexity and with plants containing recently identified R genes from related but different Solanum species. In addition, a differential set of ten Genetically Modified (GM) plants harboring single late blight R genes in the same genetic background (Desiree). By analyzing the virulence spectra of recently collected isolates using both newly described differential sets, we found that the GM Desiree differential set was more accurate for isolate virulence typing than the conventional (extended) differential set. Besides, the GM Desiree differential set was shown to be useful as trap plants to isolate novel P. infestans strains and to monitor virulence towards particular R genes in P. infestans populations `on site´. Legislative restrictions are, however, limiting the use of the GM Desiree differential set.
LanguageEnglish
Pages219-234
JournalEuphytica
Volume202
Issue number2
DOIs
Publication statusPublished - 2015

Fingerprint

Phytophthora infestans
vpr Genes
Solanum tuberosum
Virulence
virulence
monitoring
Solanum
genes
Oomycetes
Solanum demissum
Genetically Modified Plants
potatoes
Population
genetic background
traps

Keywords

  • resistance genes
  • solanum-bulbocastanum
  • disease resistance
  • durable resistance
  • rxlr effectors
  • united-states
  • pathogen
  • population
  • races
  • diversity

Cite this

@article{138d56f5f97e4477aefc0d4c2f6e0146,
title = "An updated conventional- and a novel GM potato late blight R gene differential set for virulence monitoring of Phytophthora infestans",
abstract = "Late blight is an important disease in potato that is caused by the oomycete Phytophthora infestans. In the past, Solanum demissum late blight resistance (R) genes were introgressed into cultivated potato (Solanum tuberosum). Eleven of these resistant plants were selected to characterize the virulence spectrum of individual P. infestans isolates and to monitor the dynamics of virulence in P. infestans populations. These plants are referred to as the Mastenbroek and Black differential sets. It has long been assumed that each differential plant contained one single R gene. In the current study and previous studies, however, most Mastenbroek differential plants were shown to harbor multiple R gene(s), which blurs virulence typing of late blight isolates. In order to acquire more accurate virulence profiles, we extended the Mastenbroek differential set with Solanum spp. plants harboring reduced R gene complexity and with plants containing recently identified R genes from related but different Solanum species. In addition, a differential set of ten Genetically Modified (GM) plants harboring single late blight R genes in the same genetic background (Desiree). By analyzing the virulence spectra of recently collected isolates using both newly described differential sets, we found that the GM Desiree differential set was more accurate for isolate virulence typing than the conventional (extended) differential set. Besides, the GM Desiree differential set was shown to be useful as trap plants to isolate novel P. infestans strains and to monitor virulence towards particular R genes in P. infestans populations `on site´. Legislative restrictions are, however, limiting the use of the GM Desiree differential set.",
keywords = "resistance genes, solanum-bulbocastanum, disease resistance, durable resistance, rxlr effectors, united-states, pathogen, population, races, diversity",
author = "{Suxian Zhu}, Suxian and J.H. Vossen and {Bergervoet-van Deelen}, J.E.M. and M.A. Nijenhuis and L.P. Kodde and G.J.T. Kessel and V.G.A.A. Vleeshouwers and R.G.F. Visser and E. Jacobsen",
year = "2015",
doi = "10.1007/s10681-014-1276-0",
language = "English",
volume = "202",
pages = "219--234",
journal = "Euphytica",
issn = "0014-2336",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - An updated conventional- and a novel GM potato late blight R gene differential set for virulence monitoring of Phytophthora infestans

AU - Suxian Zhu, Suxian

AU - Vossen, J.H.

AU - Bergervoet-van Deelen, J.E.M.

AU - Nijenhuis, M.A.

AU - Kodde, L.P.

AU - Kessel, G.J.T.

AU - Vleeshouwers, V.G.A.A.

AU - Visser, R.G.F.

AU - Jacobsen, E.

PY - 2015

Y1 - 2015

N2 - Late blight is an important disease in potato that is caused by the oomycete Phytophthora infestans. In the past, Solanum demissum late blight resistance (R) genes were introgressed into cultivated potato (Solanum tuberosum). Eleven of these resistant plants were selected to characterize the virulence spectrum of individual P. infestans isolates and to monitor the dynamics of virulence in P. infestans populations. These plants are referred to as the Mastenbroek and Black differential sets. It has long been assumed that each differential plant contained one single R gene. In the current study and previous studies, however, most Mastenbroek differential plants were shown to harbor multiple R gene(s), which blurs virulence typing of late blight isolates. In order to acquire more accurate virulence profiles, we extended the Mastenbroek differential set with Solanum spp. plants harboring reduced R gene complexity and with plants containing recently identified R genes from related but different Solanum species. In addition, a differential set of ten Genetically Modified (GM) plants harboring single late blight R genes in the same genetic background (Desiree). By analyzing the virulence spectra of recently collected isolates using both newly described differential sets, we found that the GM Desiree differential set was more accurate for isolate virulence typing than the conventional (extended) differential set. Besides, the GM Desiree differential set was shown to be useful as trap plants to isolate novel P. infestans strains and to monitor virulence towards particular R genes in P. infestans populations `on site´. Legislative restrictions are, however, limiting the use of the GM Desiree differential set.

AB - Late blight is an important disease in potato that is caused by the oomycete Phytophthora infestans. In the past, Solanum demissum late blight resistance (R) genes were introgressed into cultivated potato (Solanum tuberosum). Eleven of these resistant plants were selected to characterize the virulence spectrum of individual P. infestans isolates and to monitor the dynamics of virulence in P. infestans populations. These plants are referred to as the Mastenbroek and Black differential sets. It has long been assumed that each differential plant contained one single R gene. In the current study and previous studies, however, most Mastenbroek differential plants were shown to harbor multiple R gene(s), which blurs virulence typing of late blight isolates. In order to acquire more accurate virulence profiles, we extended the Mastenbroek differential set with Solanum spp. plants harboring reduced R gene complexity and with plants containing recently identified R genes from related but different Solanum species. In addition, a differential set of ten Genetically Modified (GM) plants harboring single late blight R genes in the same genetic background (Desiree). By analyzing the virulence spectra of recently collected isolates using both newly described differential sets, we found that the GM Desiree differential set was more accurate for isolate virulence typing than the conventional (extended) differential set. Besides, the GM Desiree differential set was shown to be useful as trap plants to isolate novel P. infestans strains and to monitor virulence towards particular R genes in P. infestans populations `on site´. Legislative restrictions are, however, limiting the use of the GM Desiree differential set.

KW - resistance genes

KW - solanum-bulbocastanum

KW - disease resistance

KW - durable resistance

KW - rxlr effectors

KW - united-states

KW - pathogen

KW - population

KW - races

KW - diversity

U2 - 10.1007/s10681-014-1276-0

DO - 10.1007/s10681-014-1276-0

M3 - Article

VL - 202

SP - 219

EP - 234

JO - Euphytica

T2 - Euphytica

JF - Euphytica

SN - 0014-2336

IS - 2

ER -