An intracellular pH gradient in the anammox bacterium Kuenenia stuttgartiensis as evaluated by P-31 NMR

W.R.L. van der Star, C. Dijkema, P. de Waard, C. Picioreanu, M. Strous, M.C.M. van Loosdrecht

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54 Citations (Scopus)

Abstract

The cytoplasm of anaerobic ammonium oxidizing (anammox) bacteria consists of three compartments separated by membranes. It has been suggested that a proton motive force may be generated over the membrane of the innermost compartment, the "anammoxosome". P-31 nuclear magnetic resonance (NMR) spectroscopy was employed to investigate intracellular pH differences in the anammox bacterium Kuenenia stuttgartiensis. With in vivo NMR, spectra were recorded of active, highly concentrated suspensions of K. stuttgartiensis in a wide-bore NMR tube. At different external pH values, two stable and distinct phosphate peaks were apparent in the recorded spectra. These peaks were equivalent with pH values of 7.3 and 6.3 and suggested the presence of a proton motive force over an intracytoplasmic membrane in K. stuttgartiensis. This study provides for the second time-after discovery of acidocalcisome-like compartments in Agrobacterium tumefaciens-evidence for an intracytoplasmic pH gradient in a chemotrophic prokaryotic cell
Original languageEnglish
Pages (from-to)311-317
JournalApplied Microbiology and Biotechnology
Volume86
Issue number1
DOIs
Publication statusPublished - 2010

Keywords

  • ammonium-oxidizing bacteria
  • nuclear-magnetic-resonance
  • proton gradients
  • 4 genera
  • compartmentation
  • enrichment
  • oxidation
  • reactor
  • cells
  • identification

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