An internal control for the diagnosis of Crown Gall by PCR

J. Cubero; J.M. van der Wolf; J.R.C.M. van Beckhoven; M.M. Lopez

doi:10.1016/S0167-7012(02)00153-7

An internal control for the diagnosis of Crown Gall by PCR

J. Cubero, J.M. van der Wolf, J.R.C.M. van Beckhoven, M.M. Lopez

Research output: Contribution to journal › Article › Academic › peer-review

29 Citations (Scopus)

Abstract

The addition of an internal control (IC) at the appropriate concentration enables recognize of false negatives in the detection of Agrobacterium tumefaciens and improves the reliability of PCR for crown gall diagnosis. Co-amplification of the IC and target sequence from A. tumefaciens ensures the attainment of at least one amplification product in every PCR reaction if the DNA extracted is of high enough quality to be amplified.

Original language	English
Pages (from-to)	387-392
Number of pages	5
Journal	Journal of Microbiological Methods
Volume	51
Issue number	3
DOIs	https://doi.org/10.1016/S0167-7012(02)00153-7
Publication status	Published - 2002

Keywords

polymerase-chain-reaction
phytopathogenic agrobacterium strains
plant tumors
tumefaciens
radiobacter
localization
bacteremia
extraction
disease
tissues

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10.1016/S0167-7012(02)00153-7

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title = "An internal control for the diagnosis of Crown Gall by PCR",

abstract = "The addition of an internal control (IC) at the appropriate concentration enables recognize of false negatives in the detection of Agrobacterium tumefaciens and improves the reliability of PCR for crown gall diagnosis. Co-amplification of the IC and target sequence from A. tumefaciens ensures the attainment of at least one amplification product in every PCR reaction if the DNA extracted is of high enough quality to be amplified.",

keywords = "polymerase-chain-reaction, phytopathogenic agrobacterium strains, plant tumors, tumefaciens, radiobacter, localization, bacteremia, extraction, disease, tissues",

author = "J. Cubero and {van der Wolf}, J.M. and {van Beckhoven}, J.R.C.M. and M.M. Lopez",

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T1 - An internal control for the diagnosis of Crown Gall by PCR

AU - Cubero, J.

AU - van der Wolf, J.M.

AU - van Beckhoven, J.R.C.M.

AU - Lopez, M.M.

PY - 2002

Y1 - 2002

N2 - The addition of an internal control (IC) at the appropriate concentration enables recognize of false negatives in the detection of Agrobacterium tumefaciens and improves the reliability of PCR for crown gall diagnosis. Co-amplification of the IC and target sequence from A. tumefaciens ensures the attainment of at least one amplification product in every PCR reaction if the DNA extracted is of high enough quality to be amplified.

AB - The addition of an internal control (IC) at the appropriate concentration enables recognize of false negatives in the detection of Agrobacterium tumefaciens and improves the reliability of PCR for crown gall diagnosis. Co-amplification of the IC and target sequence from A. tumefaciens ensures the attainment of at least one amplification product in every PCR reaction if the DNA extracted is of high enough quality to be amplified.

KW - polymerase-chain-reaction

KW - phytopathogenic agrobacterium strains

KW - plant tumors

KW - tumefaciens

KW - radiobacter

KW - localization

KW - bacteremia

KW - extraction

KW - disease

KW - tissues

U2 - 10.1016/S0167-7012(02)00153-7

DO - 10.1016/S0167-7012(02)00153-7

M3 - Article

SN - 0167-7012

VL - 51

SP - 387

EP - 392

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 3

ER -

An internal control for the diagnosis of Crown Gall by PCR

Abstract

Keywords

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