An internal control for the diagnosis of Crown Gall by PCR

    Research output: Contribution to journalArticleAcademicpeer-review

    29 Citations (Scopus)

    Abstract

    The addition of an internal control (IC) at the appropriate concentration enables recognize of false negatives in the detection of Agrobacterium tumefaciens and improves the reliability of PCR for crown gall diagnosis. Co-amplification of the IC and target sequence from A. tumefaciens ensures the attainment of at least one amplification product in every PCR reaction if the DNA extracted is of high enough quality to be amplified.
    Original languageEnglish
    Pages (from-to)387-392
    Number of pages5
    JournalJournal of Microbiological Methods
    Volume51
    Issue number3
    DOIs
    Publication statusPublished - 2002

    Keywords

    • polymerase-chain-reaction
    • phytopathogenic agrobacterium strains
    • plant tumors
    • tumefaciens
    • radiobacter
    • localization
    • bacteremia
    • extraction
    • disease
    • tissues

    Fingerprint Dive into the research topics of 'An internal control for the diagnosis of Crown Gall by PCR'. Together they form a unique fingerprint.

  • Cite this