The addition of an internal control (IC) at the appropriate concentration enables recognize of false negatives in the detection of Agrobacterium tumefaciens and improves the reliability of PCR for crown gall diagnosis. Co-amplification of the IC and target sequence from A. tumefaciens ensures the attainment of at least one amplification product in every PCR reaction if the DNA extracted is of high enough quality to be amplified.
- phytopathogenic agrobacterium strains
- plant tumors
Cubero, J., van der Wolf, J. M., van Beckhoven, J. R. C. M., & Lopez, M. M. (2002). An internal control for the diagnosis of Crown Gall by PCR. Journal of Microbiological Methods, 51(3), 387-392. https://doi.org/10.1016/S0167-7012(02)00153-7