An integrated QSAR-PBK/D modelling approach for predicting detoxification and DNA adduct formation of 18 acyclic food-borne a,ß-unsaturated aldehydes.

R. Kiwamoto, A. Spenkelink, I.M.C.M. Rietjens, A. Punt

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)

Abstract

Acyclic a,ß-unsaturated aldehydes present in food raise a concern because the a,ß-unsaturated aldehyde moiety is considered a structural alert for genotoxicity. However, controversy remains on whether in vivo at realistic dietary exposure DNA adduct formation is significant. The aim of the present study was to develop physiologically based kinetic/dynamic (PBK/D) models to examine dose-dependent detoxification and DNA adduct formation of a group of 18 food-borne acyclic a,ß-unsaturated aldehydes without 2- or 3-alkylation, and with no more than one conjugated double bond. Parameters for the PBK/D models were obtained using quantitative structure-activity relationships (QSARs) defined with a training set of six selected aldehydes. Using the QSARs, PBK/D models for the other 12 aldehydes were defined. Results revealed that DNA adduct formation in the liver increases with decreasing bulkiness of the molecule especially due to less efficient detoxification. 2-Propenal (acrolein) was identified to induce the highest DNA adduct levels. At realistic dietary intake, the predicted DNA adduct levels for all aldehydes were two orders of magnitude lower than endogenous background levels observed in disease free human liver, suggesting that for all 18 aldehydes DNA adduct formation is negligible at the relevant levels of dietary intake. The present study provides a proof of principle for the use of QSAR-based PBK/D modelling to facilitate group evaluations and read-across in risk assessment.
Original languageEnglish
Pages (from-to)108-117
JournalToxicology and Applied Pharmacology
Volume282
Issue number1
DOIs
Publication statusPublished - 2015

Keywords

  • in-silico model
  • exocyclic 1,n-2-propanodeoxyguanosine
  • 1,n(2)-propanodeoxyguanosine adducts
  • trans-2-hexenal detoxification
  • glutathione transferases
  • estragole bioactivation
  • pharmacokinetic model
  • carbonyl-compounds
  • human liver
  • rat

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