An integrated and optimized process for cleaner production of ethanol and biodiesel from corn stover by Mucor indicus

Razieh Shafiei Alavijeh, Keikhosro Karimi*, Corjan van den Berg

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

A two-stage process was successfully developed for biodiesel and ethanol production from corn stover using zygomycetes fungus Mucor indicus. Dilute-acid pretreatment followed by enzymatic saccharification was applied to release the maximum amount of sugars (glucose and xylose) from the lignocellulosic structure of corn stover. Dilute-acid hydrolysis was optimized by a response surface design. Under the optimal reaction conditions (i.e., 1.8% v/v H2SO4, 121 °C for 22 min), the hydrolysis resulted in the production of 270 g glucose per kg of dry corn stover (57.8% theoretical yield) and 100 g xylose per kg of dry corn stover (84.0% theoretical yield). Validation of the model exhibited proper fit between predicted and observed values of glucose and xylose concentrations: 91.4% regression adjustment for xylose and 98.2% for glucose. In the first stage, cells fermented the enzymatic hydrolysate to the maximum amount of 74.5% (0.38 g g−1) ethanol as the main product. In the second stage, the dilute-acid hydrolysate was used for lipid accumulation in the fungal cells of the first stage fermentation. The hydrolysates were used without detoxification since the fungus is among the most resistant microorganisms to the inhibitors available in the acid hydrolysates. Effects of addition of different nutrient sources, including fungal extract and yeast extract along with mineral salts, were also investigated to maximize lipid yield. Overall, 21.4 g ethanol and 2.2 g biodiesel (obtained from 4.00 g accumulated lipid) were produced from 100 g dry corn stover.

Original languageEnglish
Article number119321
JournalJournal of Cleaner Production
Volume249
DOIs
Publication statusPublished - 10 Mar 2020

Fingerprint

cleaner production
Xylose
Biodiesel
Glucose
ethanol
Ethanol
maize
Lipids
glucose
Acids
Fungi
lipid
acid
Hydrolysis
hydrolysis
Saccharification
Detoxification
fungus
Sugars
Microorganisms

Keywords

  • Biodiesel production
  • Ethanol production
  • Lignocellulosic substrate
  • Microbial lipid
  • Mucor indicus

Cite this

@article{eadbe72156504ae493120f7004d459d4,
title = "An integrated and optimized process for cleaner production of ethanol and biodiesel from corn stover by Mucor indicus",
abstract = "A two-stage process was successfully developed for biodiesel and ethanol production from corn stover using zygomycetes fungus Mucor indicus. Dilute-acid pretreatment followed by enzymatic saccharification was applied to release the maximum amount of sugars (glucose and xylose) from the lignocellulosic structure of corn stover. Dilute-acid hydrolysis was optimized by a response surface design. Under the optimal reaction conditions (i.e., 1.8{\%} v/v H2SO4, 121 °C for 22 min), the hydrolysis resulted in the production of 270 g glucose per kg of dry corn stover (57.8{\%} theoretical yield) and 100 g xylose per kg of dry corn stover (84.0{\%} theoretical yield). Validation of the model exhibited proper fit between predicted and observed values of glucose and xylose concentrations: 91.4{\%} regression adjustment for xylose and 98.2{\%} for glucose. In the first stage, cells fermented the enzymatic hydrolysate to the maximum amount of 74.5{\%} (0.38 g g−1) ethanol as the main product. In the second stage, the dilute-acid hydrolysate was used for lipid accumulation in the fungal cells of the first stage fermentation. The hydrolysates were used without detoxification since the fungus is among the most resistant microorganisms to the inhibitors available in the acid hydrolysates. Effects of addition of different nutrient sources, including fungal extract and yeast extract along with mineral salts, were also investigated to maximize lipid yield. Overall, 21.4 g ethanol and 2.2 g biodiesel (obtained from 4.00 g accumulated lipid) were produced from 100 g dry corn stover.",
keywords = "Biodiesel production, Ethanol production, Lignocellulosic substrate, Microbial lipid, Mucor indicus",
author = "{Shafiei Alavijeh}, Razieh and Keikhosro Karimi and {van den Berg}, Corjan",
year = "2020",
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language = "English",
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journal = "Journal of Cleaner Production",
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An integrated and optimized process for cleaner production of ethanol and biodiesel from corn stover by Mucor indicus. / Shafiei Alavijeh, Razieh; Karimi, Keikhosro; van den Berg, Corjan.

In: Journal of Cleaner Production, Vol. 249, 119321, 10.03.2020.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - An integrated and optimized process for cleaner production of ethanol and biodiesel from corn stover by Mucor indicus

AU - Shafiei Alavijeh, Razieh

AU - Karimi, Keikhosro

AU - van den Berg, Corjan

PY - 2020/3/10

Y1 - 2020/3/10

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AB - A two-stage process was successfully developed for biodiesel and ethanol production from corn stover using zygomycetes fungus Mucor indicus. Dilute-acid pretreatment followed by enzymatic saccharification was applied to release the maximum amount of sugars (glucose and xylose) from the lignocellulosic structure of corn stover. Dilute-acid hydrolysis was optimized by a response surface design. Under the optimal reaction conditions (i.e., 1.8% v/v H2SO4, 121 °C for 22 min), the hydrolysis resulted in the production of 270 g glucose per kg of dry corn stover (57.8% theoretical yield) and 100 g xylose per kg of dry corn stover (84.0% theoretical yield). Validation of the model exhibited proper fit between predicted and observed values of glucose and xylose concentrations: 91.4% regression adjustment for xylose and 98.2% for glucose. In the first stage, cells fermented the enzymatic hydrolysate to the maximum amount of 74.5% (0.38 g g−1) ethanol as the main product. In the second stage, the dilute-acid hydrolysate was used for lipid accumulation in the fungal cells of the first stage fermentation. The hydrolysates were used without detoxification since the fungus is among the most resistant microorganisms to the inhibitors available in the acid hydrolysates. Effects of addition of different nutrient sources, including fungal extract and yeast extract along with mineral salts, were also investigated to maximize lipid yield. Overall, 21.4 g ethanol and 2.2 g biodiesel (obtained from 4.00 g accumulated lipid) were produced from 100 g dry corn stover.

KW - Biodiesel production

KW - Ethanol production

KW - Lignocellulosic substrate

KW - Microbial lipid

KW - Mucor indicus

U2 - 10.1016/j.jclepro.2019.119321

DO - 10.1016/j.jclepro.2019.119321

M3 - Article

VL - 249

JO - Journal of Cleaner Production

JF - Journal of Cleaner Production

SN - 0959-6526

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