An improved conjugation method for Pseudomonas syringae

Helen C. Neale; Michelle T. Hulin; Richard J. Harrison; Robert W. Jackson; John W. Mansfield; Dawn L. Arnold

doi:10.1016/j.mimet.2020.106025

An improved conjugation method for Pseudomonas syringae

Helen C. Neale, Michelle T. Hulin, Richard J. Harrison, Robert W. Jackson, John W. Mansfield, Dawn L. Arnold^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

1 Citation (Scopus)

Abstract

In order to achieve saturating transposon mutagenesis of the genome of plant pathogenic strains of Pseudomonas syringae we needed to improve plasmid conjugation frequency. Manipulation of the growth stage of donor and recipient cells allowed the required increase in frequency and facilitated conjugation of otherwise recalcitrant strains.

Original language	English
Article number	106025
Journal	Journal of Microbiological Methods
Volume	177
DOIs	https://doi.org/10.1016/j.mimet.2020.106025
Publication status	Published - Oct 2020
Externally published	Yes

Keywords

Conjugation
Pseudomonas
TnSeq

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10.1016/j.mimet.2020.106025

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@article{937e617e54284abdbd8e0119bae85d26,

title = "An improved conjugation method for Pseudomonas syringae",

abstract = "In order to achieve saturating transposon mutagenesis of the genome of plant pathogenic strains of Pseudomonas syringae we needed to improve plasmid conjugation frequency. Manipulation of the growth stage of donor and recipient cells allowed the required increase in frequency and facilitated conjugation of otherwise recalcitrant strains.",

keywords = "Conjugation, Pseudomonas, TnSeq",

author = "Neale, \{Helen C.\} and Hulin, \{Michelle T.\} and Harrison, \{Richard J.\} and Jackson, \{Robert W.\} and Mansfield, \{John W.\} and Arnold, \{Dawn L.\}",

year = "2020",

month = oct,

doi = "10.1016/j.mimet.2020.106025",

language = "English",

volume = "177",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier",

}

TY - JOUR

T1 - An improved conjugation method for Pseudomonas syringae

AU - Neale, Helen C.

AU - Hulin, Michelle T.

AU - Harrison, Richard J.

AU - Jackson, Robert W.

AU - Mansfield, John W.

AU - Arnold, Dawn L.

PY - 2020/10

Y1 - 2020/10

N2 - In order to achieve saturating transposon mutagenesis of the genome of plant pathogenic strains of Pseudomonas syringae we needed to improve plasmid conjugation frequency. Manipulation of the growth stage of donor and recipient cells allowed the required increase in frequency and facilitated conjugation of otherwise recalcitrant strains.

AB - In order to achieve saturating transposon mutagenesis of the genome of plant pathogenic strains of Pseudomonas syringae we needed to improve plasmid conjugation frequency. Manipulation of the growth stage of donor and recipient cells allowed the required increase in frequency and facilitated conjugation of otherwise recalcitrant strains.

KW - Conjugation

KW - Pseudomonas

KW - TnSeq

U2 - 10.1016/j.mimet.2020.106025

DO - 10.1016/j.mimet.2020.106025

M3 - Article

C2 - 32795634

AN - SCOPUS:85089736396

SN - 0167-7012

VL - 177

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

M1 - 106025

ER -

An improved conjugation method for Pseudomonas syringae

Abstract

Keywords

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