An improved conjugation method for Pseudomonas syringae

Helen C. Neale, Michelle T. Hulin, Richard J. Harrison, Robert W. Jackson, John W. Mansfield, Dawn L. Arnold*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)


In order to achieve saturating transposon mutagenesis of the genome of plant pathogenic strains of Pseudomonas syringae we needed to improve plasmid conjugation frequency. Manipulation of the growth stage of donor and recipient cells allowed the required increase in frequency and facilitated conjugation of otherwise recalcitrant strains.

Original languageEnglish
Article number106025
JournalJournal of Microbiological Methods
Publication statusPublished - Oct 2020
Externally publishedYes


  • Conjugation
  • Pseudomonas
  • TnSeq


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