An enzyme with rhamnogalacturonase activity

L.V. Kofod (Inventor), L.N. Andersen (Inventor), H. Dalboge (Inventor), M.S. Kauppinen (Inventor), S. Christgau (Inventor), H.P. Heldt-Hansen (Inventor), C. Christophersen (Inventor), P.M. Nielsen (Inventor), A.G.J. Voragen (Inventor), H.A. Schols (Inventor)

Research output: Patent

Abstract

An enzyme exhibiting rhamnogalacturonase activity, which enzyme: a) is encoded by the DNA sequence shown in SEQ ID No. 1 or a sequence homologous thereto encoding a polypeptide with RGase activity, b) has the amino acid sequence shown in SEQ ID No. 2 or an analogue thereof, c) is reactive with an antibody raised against the enzyme encoded by the DNA sequence shown in SEQ ID No. 1, d) has a pH optimum above pH 5, and/or e) has a relative activity of at least 30% at a pH in the range of 5.5-6.5. The enzyme may be used for reducing the viscosity of a plant cell wall material and for extracting high molecular weight molecules from a plant cell wall material.

Original languageEnglish
Patent numberWO9420612
Priority date5/03/93
Publication statusPublished - 15 Sep 1994

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