An enzyme with rhamnogalacturonase activity

L.V. Kofod; L.N. Andersen; H. Dalboge; M.S. Kauppinen; S. Christgau; H.P. Heldt-Hansen; C. Christophersen; P.M. Nielsen; A.G.J. Voragen; H.A. Schols

An enzyme with rhamnogalacturonase activity

L.V. Kofod (Inventor), L.N. Andersen (Inventor), H. Dalboge (Inventor), M.S. Kauppinen (Inventor), S. Christgau (Inventor), H.P. Heldt-Hansen (Inventor), C. Christophersen (Inventor), P.M. Nielsen (Inventor), A.G.J. Voragen (Inventor), H.A. Schols (Inventor)

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Research output: Patent

Abstract

An enzyme exhibiting rhamnogalacturonase activity, which enzyme: a) is encoded by the DNA sequence shown in SEQ ID No. 1 or a sequence homologous thereto encoding a polypeptide with RGase activity, b) has the amino acid sequence shown in SEQ ID No. 2 or an analogue thereof, c) is reactive with an antibody raised against the enzyme encoded by the DNA sequence shown in SEQ ID No. 1, d) has a pH optimum above pH 5, and/or e) has a relative activity of at least 30% at a pH in the range of 5.5-6.5. The enzyme may be used for reducing the viscosity of a plant cell wall material and for extracting high molecular weight molecules from a plant cell wall material.

Original language	English
Patent number	WO9420612
Priority date	5/03/93
Publication status	Published - 15 Sept 1994

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Kofod, L. V., Andersen, L. N., Dalboge, H., Kauppinen, M. S., Christgau, S., Heldt-Hansen, H. P., Christophersen, C., Nielsen, P. M., Voragen, A. G. J., & Schols, H. A. (1994). An enzyme with rhamnogalacturonase activity. (Patent No. WO9420612). https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=WO&NR=9420612A1&KC=A1&FT=D&ND=1&date=19940915&DB=&locale=en_ep

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title = "An enzyme with rhamnogalacturonase activity",

abstract = "An enzyme exhibiting rhamnogalacturonase activity, which enzyme: a) is encoded by the DNA sequence shown in SEQ ID No. 1 or a sequence homologous thereto encoding a polypeptide with RGase activity, b) has the amino acid sequence shown in SEQ ID No. 2 or an analogue thereof, c) is reactive with an antibody raised against the enzyme encoded by the DNA sequence shown in SEQ ID No. 1, d) has a pH optimum above pH 5, and/or e) has a relative activity of at least 30% at a pH in the range of 5.5-6.5. The enzyme may be used for reducing the viscosity of a plant cell wall material and for extracting high molecular weight molecules from a plant cell wall material.",

author = "L.V. Kofod and L.N. Andersen and H. Dalboge and M.S. Kauppinen and S. Christgau and H.P. Heldt-Hansen and C. Christophersen and P.M. Nielsen and A.G.J. Voragen and H.A. Schols",

note = "aanvrager: NOVO Nordisk A/S, Bagsvaerd, Denmark; WO9420612",

year = "1994",

month = sep,

day = "15",

language = "English",

type = "Patent",

}

TY - PAT

T1 - An enzyme with rhamnogalacturonase activity

AU - Kofod, L.V.

AU - Andersen, L.N.

AU - Dalboge, H.

AU - Kauppinen, M.S.

AU - Christgau, S.

AU - Heldt-Hansen, H.P.

AU - Christophersen, C.

AU - Nielsen, P.M.

AU - Voragen, A.G.J.

AU - Schols, H.A.

N1 - aanvrager: NOVO Nordisk A/S, Bagsvaerd, Denmark

PY - 1994/9/15

Y1 - 1994/9/15

N2 - An enzyme exhibiting rhamnogalacturonase activity, which enzyme: a) is encoded by the DNA sequence shown in SEQ ID No. 1 or a sequence homologous thereto encoding a polypeptide with RGase activity, b) has the amino acid sequence shown in SEQ ID No. 2 or an analogue thereof, c) is reactive with an antibody raised against the enzyme encoded by the DNA sequence shown in SEQ ID No. 1, d) has a pH optimum above pH 5, and/or e) has a relative activity of at least 30% at a pH in the range of 5.5-6.5. The enzyme may be used for reducing the viscosity of a plant cell wall material and for extracting high molecular weight molecules from a plant cell wall material.

AB - An enzyme exhibiting rhamnogalacturonase activity, which enzyme: a) is encoded by the DNA sequence shown in SEQ ID No. 1 or a sequence homologous thereto encoding a polypeptide with RGase activity, b) has the amino acid sequence shown in SEQ ID No. 2 or an analogue thereof, c) is reactive with an antibody raised against the enzyme encoded by the DNA sequence shown in SEQ ID No. 1, d) has a pH optimum above pH 5, and/or e) has a relative activity of at least 30% at a pH in the range of 5.5-6.5. The enzyme may be used for reducing the viscosity of a plant cell wall material and for extracting high molecular weight molecules from a plant cell wall material.

M3 - Patent

M1 - WO9420612

ER -

An enzyme with rhamnogalacturonase activity

Abstract

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