An efficient method for DNA extraction from Cladosporioid fungi

M.A. Moslem, A.H. Bahkali, K.A. Abd-Elsalam, P.J.G.M. de Wit

Research output: Contribution to journalArticleAcademicpeer-review

16 Citations (Scopus)

Abstract

We developed an efficient method for DNA extraction from Cladosporioid fungi, which are important fungal plant pathogens. The cell wall of Cladosporioid fungi is often melanized, which makes it difficult to extract DNA from their cells. In order to overcome this we grew these fungi for three days on agar plates and extracted DNA from mycelium mats after manual or electric homogenization. High-quality DNA was isolated, with an A260/A280 ratio ranging between 1.6 and 2.0. Isolated genomic DNA was efficiently digested with restriction enzymes and produced distinct banding patterns on agarose gels for the different Cladosporium species. Clear DNA fragments from the isolated DNA were amplified by PCR using small and large subunit rDNA primers, demonstrating that this method provides DNA of sufficiently high quality for molecular analyses
Original languageEnglish
Pages (from-to)2283-2291
JournalGenetics and Molecular Research
Volume9
Issue number4
DOIs
Publication statusPublished - 2010

Keywords

  • candida-albicans
  • pcr
  • amplification
  • pathogens
  • diagnosis

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