An efficient enzymatic synthesis of 5-aminovaleric acid

A. Pukin, C.G. Boeriu, E.L. Scott, J.P.M. Sanders, M.C.R. Franssen

Research output: Contribution to journalArticleAcademicpeer-review

31 Citations (Scopus)

Abstract

The title compound was prepared enzymatically from l-lysine in an excellent yield and under buffer-free conditions. l-Lysine was oxidized by the action of l-lysine a-oxidase from Trichoderma viride followed by spontaneous oxidative decarboxylation of the intermediate 6-amino-2-oxocaproic acid in the reaction medium. l-Lysine a-oxidase was immobilized on an epoxy-activated solid support (Sepabeads EC-EP) and the activity of both solution-based and immobilized enzyme in this reaction was determined.
Original languageEnglish
Pages (from-to)58-62
JournalJournal of Molecular Catalysis. B, Enzymatic
Volume65
Issue number1-4
DOIs
Publication statusPublished - 2010

Keywords

  • lysine alpha-oxidase
  • amperometric biosensor
  • amino-acids
  • immobilization
  • electrode
  • sepabeads
  • stability
  • proteins

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