An analysis of characterized plant sesquiterpene synthases

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

Plants exhibit a vast array of sesquiterpenes, C15 hydrocarbons which often function as herbivore-repellents or pollinator-attractants. These in turn are produced by a diverse range of sesquiterpene synthases. A comprehensive analysis of these enzymes in terms of product specificity has been hampered by the lack of a centralized resource of sufficient functionally annotated sequence data. To address this, we have gathered 262 plant sesquiterpene synthase sequences with experimentally characterized products. The annotated enzyme sequences allowed for an analysis of terpene synthase motifs, leading to the extension of one motif and recognition of a variant of another. In addition, putative terpene synthase sequences were obtained from various resources and compared with the annotated sesquiterpene synthases. This analysis indicated regions of terpene synthase sequence space which so far are unexplored experimentally. Finally, we present a case describing mutational studies on residues altering product specificity, for which we analyzed conservation in our database. This demonstrates an application of our database in choosing likely-functional residues for mutagenesis studies aimed at understanding or changing sesquiterpene synthase product specificity.

Original languageEnglish
Pages (from-to)157-165
JournalPhytochemistry
Volume158
Early online date13 Nov 2018
DOIs
Publication statusPublished - Feb 2019

Fingerprint

Sesquiterpenes
sesquiterpenoids
terpenoids
Databases
Mutagenesis
Herbivory
attractants
Enzymes
repellents
Hydrocarbons
enzymes
mutagenesis
pollinators
Sequence Analysis
hydrocarbons
Conservation
herbivores
sequence analysis
terpene synthase

Keywords

  • Database
  • Enzyme
  • Product specificity
  • Sesquiterpene
  • Sesquiterpene synthase
  • Terpene synthase

Cite this

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title = "An analysis of characterized plant sesquiterpene synthases",
abstract = "Plants exhibit a vast array of sesquiterpenes, C15 hydrocarbons which often function as herbivore-repellents or pollinator-attractants. These in turn are produced by a diverse range of sesquiterpene synthases. A comprehensive analysis of these enzymes in terms of product specificity has been hampered by the lack of a centralized resource of sufficient functionally annotated sequence data. To address this, we have gathered 262 plant sesquiterpene synthase sequences with experimentally characterized products. The annotated enzyme sequences allowed for an analysis of terpene synthase motifs, leading to the extension of one motif and recognition of a variant of another. In addition, putative terpene synthase sequences were obtained from various resources and compared with the annotated sesquiterpene synthases. This analysis indicated regions of terpene synthase sequence space which so far are unexplored experimentally. Finally, we present a case describing mutational studies on residues altering product specificity, for which we analyzed conservation in our database. This demonstrates an application of our database in choosing likely-functional residues for mutagenesis studies aimed at understanding or changing sesquiterpene synthase product specificity.",
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author = "Janani Durairaj and {Di Girolamo}, Alice and Bouwmeester, {Harro J.} and {de Ridder}, Dick and Jules Beekwilder and {van Dijk}, {Aalt D.J.}",
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language = "English",
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pages = "157--165",
journal = "Phytochemistry",
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An analysis of characterized plant sesquiterpene synthases. / Durairaj, Janani; Di Girolamo, Alice; Bouwmeester, Harro J.; de Ridder, Dick; Beekwilder, Jules; van Dijk, Aalt D.J.

In: Phytochemistry, Vol. 158, 02.2019, p. 157-165.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - An analysis of characterized plant sesquiterpene synthases

AU - Durairaj, Janani

AU - Di Girolamo, Alice

AU - Bouwmeester, Harro J.

AU - de Ridder, Dick

AU - Beekwilder, Jules

AU - van Dijk, Aalt D.J.

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AB - Plants exhibit a vast array of sesquiterpenes, C15 hydrocarbons which often function as herbivore-repellents or pollinator-attractants. These in turn are produced by a diverse range of sesquiterpene synthases. A comprehensive analysis of these enzymes in terms of product specificity has been hampered by the lack of a centralized resource of sufficient functionally annotated sequence data. To address this, we have gathered 262 plant sesquiterpene synthase sequences with experimentally characterized products. The annotated enzyme sequences allowed for an analysis of terpene synthase motifs, leading to the extension of one motif and recognition of a variant of another. In addition, putative terpene synthase sequences were obtained from various resources and compared with the annotated sesquiterpene synthases. This analysis indicated regions of terpene synthase sequence space which so far are unexplored experimentally. Finally, we present a case describing mutational studies on residues altering product specificity, for which we analyzed conservation in our database. This demonstrates an application of our database in choosing likely-functional residues for mutagenesis studies aimed at understanding or changing sesquiterpene synthase product specificity.

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KW - Product specificity

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