An accurate in vitro assay for high-throughput disease testing of Phytophthora infestans in potato

Research output: Contribution to journalArticleAcademicpeer-review

22 Citations (Scopus)

Abstract

Research and breeding for resistance to Phytophthora infestans, the causal agent of the devastating late blight disease in potato (Solanum tuberosum), requires extensive disease assessments. Especially for large-scale experiments such as recombinant screenings for genetic mapping of novel resistance genes, a quick and space-effective method is required. Potato breeding clones and collections of wild Solanum germ plasm are often maintained in vitro, and this environment is extremely favorable for late blight disease development. In this paper, we describe the development of a high-throughput in vitro disease testing assay. We compared the new method with the well-established detached-leaf assay and proved that the in vitro assay is accurate and reliable, and can routinely be used for investigating the qualitative interaction between potato and P. infestans
Original languageEnglish
Pages (from-to)1263-1267
JournalPlant Disease
Volume89
Issue number12
DOIs
Publication statusPublished - 2005

Keywords

  • broad-spectrum resistance
  • major gene resistance
  • late blight
  • solanum-bulbocastanum
  • complementary races
  • bary
  • fungus
  • mont

Fingerprint Dive into the research topics of 'An accurate in vitro assay for high-throughput disease testing of Phytophthora infestans in potato'. Together they form a unique fingerprint.

Cite this