An 8-Week High-Fat Diet Induces Obesity and Insulin Resistance with Small Changes in the Muscle Transcriptome of C57BL/6J Mice

J. de Wilde; E. Smit; R. Mohren; M.V. Boekschoten; P.J. de Groot; S.A.A. van den Berg; S. Bijland; P.J. Voshol; K. Willems van Dijk; N.J.W. de Wit; A. Bunschoten; G. Schaart; M.F.M. Hulshof; E.C.M. Mariman

doi:10.1159/000308466

An 8-Week High-Fat Diet Induces Obesity and Insulin Resistance with Small Changes in the Muscle Transcriptome of C57BL/6J Mice

J. de Wilde, E. Smit, R. Mohren, M.V. Boekschoten, P.J. de Groot, S.A.A. van den Berg, S. Bijland, P.J. Voshol, K. Willems van Dijk, N.J.W. de Wit, A. Bunschoten, G. Schaart, M.F.M. Hulshof, E.C.M. Mariman

Research output: Contribution to journal › Article › Academic › peer-review

28 Citations (Scopus)

Abstract

Background: Skeletal muscle is responsible for most of the insulin-stimulated glucose uptake and metabolism. Therefore, it plays an important role in the development of insulin resistance, one of the characteristics of the metabolic syndrome (MS). As the prevalence of the MS is increasing, there is an urgent need for more effective intervention strategies. Methods: C57BL/6J mice were fed an 8-week low-fat diet (10 kcal%; LFD) or high-fat diet (45 kcal%; HFD). Microarray analysis was performed by using two comparisons: (1) 8-week HFD transcriptome versus 8-week LFD transcriptome and (2) transcriptome of mice sacrificed at the start of the intervention versus 8-week LFD transcriptome and 8-week HFD transcriptome, respectively. Results: Although an 8-week HFD induced obesity and impaired insulin sensitivity, HFD-responsive changes in the muscle transcriptome were relatively small (

Original language	English
Pages (from-to)	280-291
Journal	Journal of Nutrigenetics and Nutrigenomics
Volume	2
Issue number	6
DOIs	https://doi.org/10.1159/000308466
Publication status	Published - 2009

Keywords

glucose-transporter expression
skeletal-muscle
metabolic syndrome
adipose-tissue
triglyceride content
oxidative capacity
protein-kinases
lipid-content
sensitivity
cascade

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1159/000308466

Cite this

de Wilde, J., Smit, E., Mohren, R., Boekschoten, M. V., de Groot, P. J., van den Berg, S. A. A., Bijland, S., Voshol, P. J., Willems van Dijk, K., de Wit, N. J. W., Bunschoten, A., Schaart, G., Hulshof, M. F. M., & Mariman, E. C. M. (2009). An 8-Week High-Fat Diet Induces Obesity and Insulin Resistance with Small Changes in the Muscle Transcriptome of C57BL/6J Mice. Journal of Nutrigenetics and Nutrigenomics, 2(6), 280-291. https://doi.org/10.1159/000308466

@article{96af6f17811646e2b06e392fed352ae4,

title = "An 8-Week High-Fat Diet Induces Obesity and Insulin Resistance with Small Changes in the Muscle Transcriptome of C57BL/6J Mice",

abstract = "Background: Skeletal muscle is responsible for most of the insulin-stimulated glucose uptake and metabolism. Therefore, it plays an important role in the development of insulin resistance, one of the characteristics of the metabolic syndrome (MS). As the prevalence of the MS is increasing, there is an urgent need for more effective intervention strategies. Methods: C57BL/6J mice were fed an 8-week low-fat diet (10 kcal%; LFD) or high-fat diet (45 kcal%; HFD). Microarray analysis was performed by using two comparisons: (1) 8-week HFD transcriptome versus 8-week LFD transcriptome and (2) transcriptome of mice sacrificed at the start of the intervention versus 8-week LFD transcriptome and 8-week HFD transcriptome, respectively. Results: Although an 8-week HFD induced obesity and impaired insulin sensitivity, HFD-responsive changes in the muscle transcriptome were relatively small (",

keywords = "glucose-transporter expression, skeletal-muscle, metabolic syndrome, adipose-tissue, triglyceride content, oxidative capacity, protein-kinases, lipid-content, sensitivity, cascade",

author = "{de Wilde}, J. and E. Smit and R. Mohren and M.V. Boekschoten and {de Groot}, P.J. and {van den Berg}, S.A.A. and S. Bijland and P.J. Voshol and {Willems van Dijk}, K. and {de Wit}, N.J.W. and A. Bunschoten and G. Schaart and M.F.M. Hulshof and E.C.M. Mariman",

year = "2009",

doi = "10.1159/000308466",

language = "English",

volume = "2",

pages = "280--291",

journal = "Journal of Nutrigenetics and Nutrigenomics",

issn = "1661-6499",

publisher = "Karger",

number = "6",

}

de Wilde, J, Smit, E, Mohren, R, Boekschoten, MV, de Groot, PJ, van den Berg, SAA, Bijland, S, Voshol, PJ, Willems van Dijk, K, de Wit, NJW , Bunschoten, A, Schaart, G, Hulshof, MFM & Mariman, ECM 2009, 'An 8-Week High-Fat Diet Induces Obesity and Insulin Resistance with Small Changes in the Muscle Transcriptome of C57BL/6J Mice', Journal of Nutrigenetics and Nutrigenomics, vol. 2, no. 6, pp. 280-291. https://doi.org/10.1159/000308466

TY - JOUR

T1 - An 8-Week High-Fat Diet Induces Obesity and Insulin Resistance with Small Changes in the Muscle Transcriptome of C57BL/6J Mice

AU - de Wilde, J.

AU - Smit, E.

AU - Mohren, R.

AU - Boekschoten, M.V.

AU - de Groot, P.J.

AU - van den Berg, S.A.A.

AU - Bijland, S.

AU - Voshol, P.J.

AU - Willems van Dijk, K.

AU - de Wit, N.J.W.

AU - Bunschoten, A.

AU - Schaart, G.

AU - Hulshof, M.F.M.

AU - Mariman, E.C.M.

PY - 2009

Y1 - 2009

N2 - Background: Skeletal muscle is responsible for most of the insulin-stimulated glucose uptake and metabolism. Therefore, it plays an important role in the development of insulin resistance, one of the characteristics of the metabolic syndrome (MS). As the prevalence of the MS is increasing, there is an urgent need for more effective intervention strategies. Methods: C57BL/6J mice were fed an 8-week low-fat diet (10 kcal%; LFD) or high-fat diet (45 kcal%; HFD). Microarray analysis was performed by using two comparisons: (1) 8-week HFD transcriptome versus 8-week LFD transcriptome and (2) transcriptome of mice sacrificed at the start of the intervention versus 8-week LFD transcriptome and 8-week HFD transcriptome, respectively. Results: Although an 8-week HFD induced obesity and impaired insulin sensitivity, HFD-responsive changes in the muscle transcriptome were relatively small (

AB - Background: Skeletal muscle is responsible for most of the insulin-stimulated glucose uptake and metabolism. Therefore, it plays an important role in the development of insulin resistance, one of the characteristics of the metabolic syndrome (MS). As the prevalence of the MS is increasing, there is an urgent need for more effective intervention strategies. Methods: C57BL/6J mice were fed an 8-week low-fat diet (10 kcal%; LFD) or high-fat diet (45 kcal%; HFD). Microarray analysis was performed by using two comparisons: (1) 8-week HFD transcriptome versus 8-week LFD transcriptome and (2) transcriptome of mice sacrificed at the start of the intervention versus 8-week LFD transcriptome and 8-week HFD transcriptome, respectively. Results: Although an 8-week HFD induced obesity and impaired insulin sensitivity, HFD-responsive changes in the muscle transcriptome were relatively small (

KW - glucose-transporter expression

KW - skeletal-muscle

KW - metabolic syndrome

KW - adipose-tissue

KW - triglyceride content

KW - oxidative capacity

KW - protein-kinases

KW - lipid-content

KW - sensitivity

KW - cascade

U2 - 10.1159/000308466

DO - 10.1159/000308466

M3 - Article

SN - 1661-6499

VL - 2

SP - 280

EP - 291

JO - Journal of Nutrigenetics and Nutrigenomics

JF - Journal of Nutrigenetics and Nutrigenomics

IS - 6

ER -

An 8-Week High-Fat Diet Induces Obesity and Insulin Resistance with Small Changes in the Muscle Transcriptome of C57BL/6J Mice

Abstract

Keywords

UN SDGs

Access to Document

Fingerprint

Cite this