Abstract
The major damage induced in DNA by ultraviolet light is the induction of cyclobutane pyrimidine dimers (CPDs). Amsacta moorei entomopoxvirus (AMEV) encodes a CPD photolyase (AMV025) with a putative role in converting these dimers back into monomers. In infected Lymantria dispar cells transcription of the AMV025 gene started 8 h post inoculation (p.i.) and continued through 38 h p.i. Transcription was inhibited by a DNA synthesis blocker. Transient expression in an Escherichia coil strain that lacks its endogenous photolyase, rescued growth of the UV-irradiated bacteria in a light-dependent manner, showing that AMV025 encodes a functional DNA photolyase
Original language | English |
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Pages (from-to) | 363-365 |
Journal | Journal of Invertebrate Pathology |
Volume | 105 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2010 |
Keywords
- chrysodeixis-chalcites
- fowlpox virus
- genome sequence
- cpd-photolyase
- repair enzyme
- baculovirus
- gene
- nucleopolyhedrovirus
- identification
- cryptochromes