Carbon nanoparticles (CNPs) labeled with reporter molecules can serve as signaling labels in rapid diagnostic assays as an alternative to gold, colored latex, silica, quantum dots, or up-converting phosphor nanoparticles. Detailed here is the preparation of biomolecule-labeled CNPs and examples of their use as a versatile label. CNPs can be loaded with a range of biomolecules, such as DNA, antibodies, and proteins (e.g., neutravidin or a fusion protein of neutravidin with an enzyme), and the resulting conjugates can be used to detect analytes of high or low molecular mass.
- lateral flow immunoassay
- computer image-analysis
- fungal alpha-amylase
- immunochromatographic strip
Posthuma-Trumpie, G. A., Wichers, J. H., Koets, M., Berendsen, L. B. J. M., & van Amerongen, A. (2012). Amorphous carbon nanoparticles: a versatile label for rapid diagnostic (immuno)assays. Analytical and Bioanalytical Chemistry, 402(2), 593-600. https://doi.org/10.1007/s00216-011-5340-5