Amorphous carbon nanoparticles: a versatile label for rapid diagnostic (immuno)assays

G.A. Posthuma-Trumpie, J.H. Wichers, M. Koets, L.B.J.M. Berendsen, A. van Amerongen

Research output: Contribution to journalArticleAcademicpeer-review

81 Citations (Scopus)

Abstract

Carbon nanoparticles (CNPs) labeled with reporter molecules can serve as signaling labels in rapid diagnostic assays as an alternative to gold, colored latex, silica, quantum dots, or up-converting phosphor nanoparticles. Detailed here is the preparation of biomolecule-labeled CNPs and examples of their use as a versatile label. CNPs can be loaded with a range of biomolecules, such as DNA, antibodies, and proteins (e.g., neutravidin or a fusion protein of neutravidin with an enzyme), and the resulting conjugates can be used to detect analytes of high or low molecular mass.
Original languageEnglish
Pages (from-to)593-600
JournalAnalytical and Bioanalytical Chemistry
Volume402
Issue number2
DOIs
Publication statusPublished - 2012

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Keywords

  • lateral flow immunoassay
  • computer image-analysis
  • fungal alpha-amylase
  • immunochromatographic strip
  • escherichia-coli
  • aqueous-solution
  • assay
  • nanotubes
  • antigen
  • spp.

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