Amine Metabolism Is Influenced by Dietary Protein Source

Soumya K. Kar, Alfons J.M. Jansman, Dirkjan Schokker, Leo Kruijt, Amy C. Harms, Jerry M. Wells, Mari A. Smits

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Growth in world population will inevitably leads to increased demand for protein for humans and animals. Protein from insects and blood plasma are being considered as possible alternatives, but more research on their nutritional quality and health effects is needed. Here, we studied the effect of dietary protein source on metabolism and metabolic amine profiles in serum and urine of mice. Groups of mice were fed semi-purified diets containing 300 g/kg of soybean meal, casein, partially delactosed whey powder, spray-dried plasma
protein, wheat gluten meal, and yellow mealworm. Feed and water intake as well as body weight gain were measured for 28 days. After 14 and 28 days, serum and urine samples were collected for measurement of a large panel of amine metabolites. MetaboAnalyst 3.0 was used for analysis of the raw metabolic data. Out of 68 targeted amine metabolites, we could detect 54 in urine and 41 in blood serum. Dietary protein sources were found to have profound effects on host metabolism, particularly in systemic amine profiles, considered here as an endophenotype. We recommend serum over urine to screen for the amine metabolic endophenotype based on partial least squares discriminant analysis. We concluded that metabolites like alpha-aminobutyric acid and methylhistidine are sensitive indicators of too much or too little availability of specific amino acids in the different protein diets. Furthermore, we concluded that amine metabolic profiles can be useful for assessing the nutritional quality of different protein sources.
Original languageEnglish
Article number41
JournalFrontiers in Nutrition
Volume4
DOIs
Publication statusPublished - 1 Sep 2017

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amines
protein sources
dietary protein
blood serum
metabolism
urine
metabolites
alpha-aminobutyric acid
nutritive value
whey powder
Tenebrio molitor
wheat gluten
mice
blood plasma
discriminant analysis
soybean meal
least squares
casein
proteins
weight gain

Cite this

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title = "Amine Metabolism Is Influenced by Dietary Protein Source",
abstract = "Growth in world population will inevitably leads to increased demand for protein for humans and animals. Protein from insects and blood plasma are being considered as possible alternatives, but more research on their nutritional quality and health effects is needed. Here, we studied the effect of dietary protein source on metabolism and metabolic amine profiles in serum and urine of mice. Groups of mice were fed semi-purified diets containing 300 g/kg of soybean meal, casein, partially delactosed whey powder, spray-dried plasmaprotein, wheat gluten meal, and yellow mealworm. Feed and water intake as well as body weight gain were measured for 28 days. After 14 and 28 days, serum and urine samples were collected for measurement of a large panel of amine metabolites. MetaboAnalyst 3.0 was used for analysis of the raw metabolic data. Out of 68 targeted amine metabolites, we could detect 54 in urine and 41 in blood serum. Dietary protein sources were found to have profound effects on host metabolism, particularly in systemic amine profiles, considered here as an endophenotype. We recommend serum over urine to screen for the amine metabolic endophenotype based on partial least squares discriminant analysis. We concluded that metabolites like alpha-aminobutyric acid and methylhistidine are sensitive indicators of too much or too little availability of specific amino acids in the different protein diets. Furthermore, we concluded that amine metabolic profiles can be useful for assessing the nutritional quality of different protein sources.",
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Amine Metabolism Is Influenced by Dietary Protein Source. / Kar, Soumya K.; Jansman, Alfons J.M.; Schokker, Dirkjan; Kruijt, Leo; Harms, Amy C.; Wells, Jerry M.; Smits, Mari A.

In: Frontiers in Nutrition, Vol. 4, 41, 01.09.2017.

Research output: Contribution to journalArticleAcademicpeer-review

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