Amaryllidaceae alkaloids: identification and partial characterization of montanine production in Rhodophiala bifida plant

Andressa Reis, Kevin Magne, Sophie Massot, Luciana R. Tallini, Marina Scopel, Jaume Bastida, Pascal Ratet*, José A.S. Zuanazzi

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)

Abstract

Rhodophiala bifida (R. bifida) is a representative of the Amaryllidaceae plant family and is rich in montanine, an alkaloid with high pharmaceutical potential. Despite the interest in these compounds, many steps of the biosynthetic pathway have not been elucidated. In this study, we identified the alkaloids produced in different organs of R. bifida under different growth conditions, set up the conditions for in vitro R. bifida regeneration and initiated the molecular characterization of two R. bifida genes involved in alkaloids biosynthesis: the Norbelladine 4′-O-Methyltransferase (RbN4OMT) and the Cytochrome P450 (RbCYP96T). We show that montanine is the main alkaloid produced in the different R. bifida organs and developed a direct organogenesis regeneration protocol, using twin-scale explants cultivated on media enriched with naphthalene acetic acid and benzyladenine. Finally, we analyzed the RbN4OMT and RbCYP96T gene expressions in different organs and culture conditions and compared them to alkaloid production. In different organs of R. bifida young, adult and regenerated plants, as well as under various growing conditions, the transcripts accumulation was correlated with the production of alkaloids. This work provides new tools to improve the production of this important pharmaceutical compound and for future biotechnological studies.

Original languageEnglish
Article number8471
JournalScientific Reports
Volume9
Issue number1
DOIs
Publication statusPublished - 1 Dec 2019

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  • Research Output

    • 8 Citations
    • 1 Comment/Letter to the editor

    Author Correction: Amaryllidaceae alkaloids: identification and partial characterization of montanine production in Rhodophiala bifida plant

    Reis, A., Magne, K., Massot, S., Tallini, L. R., Scopel, M., Bastida, J., Ratet, P. & Zuanazzi, J. A. S., 1 Dec 2019, In : Scientific Reports. 9, 1, 15797.

    Research output: Contribution to journalComment/Letter to the editorAcademic

    Open Access
  • Cite this

    Reis, A., Magne, K., Massot, S., Tallini, L. R., Scopel, M., Bastida, J., Ratet, P., & Zuanazzi, J. A. S. (2019). Amaryllidaceae alkaloids: identification and partial characterization of montanine production in Rhodophiala bifida plant. Scientific Reports, 9(1), [8471]. https://doi.org/10.1038/s41598-019-44746-7