Alternative approaches can greatly reduce the number of fish used for acute toxicity testing

C.C. Hoekzema, A.J. Murk, B.J. van de Waart, J.C.M. van der Hoeven, D.F. de Roode

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

We first examined the uptake kinetics of Cd and Zn in the juvenile marine black sea bream (Acanthopagrus schlegeli) over a wide range of ambient Cd and Zn concentrations, and the relationships with metal accumulation (uptake rate and amount of nonexchangeable surface binding) were established for different fish tissues. Both Cd and Zn accumulation in the body increased linearly with exposure time after the initial metal surface binding. The dissolved Cd and Zn uptake rate constants were 2.64 and 6.50 L/kg/d, respectively, and the kinetics followed a first-order process. No evidence of biphasic transport was found, in contrast to the situation in freshwater fish. Viscera were the most important sites of metal uptake, and gills were the second most important sites. The black sea breams were then acclimated at different Cd or Zn concentrations from either waterborne or dietary source for one week, and the alteration of metal uptake kinetics or subcellular distribution and metallothionein (MT) induction were further quantified. The Cd body burden was enhanced up to 8.6- and 49-fold after waterborne and dietary Cd pre-exposure, respectively. Cadmium pre-exposure also altered the tissue-specific subcellular Cd distribution and significantly elevated tissue MT levels. In contrast, the black sea breams were able to regulate Zn accumulation, and waterborne or dietary Zn pre-exposure had only weak influences on Zn body burden and redistribution. Both Cd and Zn pre-exposures enhanced the metal uptake rate constants, whereas the nonexchangeable surface bindings were less impacted by these pre-exposures. We demonstrated a positive relationship between the Cd uptake rate and Cd or MT concentration in the fish. Pre-exposure to metals may substantially modify the kinetics of metal uptake.
LanguageEnglish
Pages1322-1325
JournalEnvironmental Toxicology and Chemistry
Volume25
Issue number5
DOIs
Publication statusPublished - 2006

Fingerprint

Fish
Toxicity
Fishes
Metals
toxicity
Black Sea
metal
Testing
fish
metallothionein
Metallothionein
Body Burden
kinetics
Kinetics
Tissue
Rate constants
Sea Bream
Viscera
exposure
Fresh Water

Keywords

  • toxicity
  • chemicals
  • tests
  • toxicology
  • testing
  • fishes
  • european-union
  • ecotoxicity
  • strategy
  • daphnia
  • embryo
  • algae

Cite this

Hoekzema, C.C. ; Murk, A.J. ; van de Waart, B.J. ; van der Hoeven, J.C.M. ; de Roode, D.F. / Alternative approaches can greatly reduce the number of fish used for acute toxicity testing. In: Environmental Toxicology and Chemistry. 2006 ; Vol. 25, No. 5. pp. 1322-1325.
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abstract = "We first examined the uptake kinetics of Cd and Zn in the juvenile marine black sea bream (Acanthopagrus schlegeli) over a wide range of ambient Cd and Zn concentrations, and the relationships with metal accumulation (uptake rate and amount of nonexchangeable surface binding) were established for different fish tissues. Both Cd and Zn accumulation in the body increased linearly with exposure time after the initial metal surface binding. The dissolved Cd and Zn uptake rate constants were 2.64 and 6.50 L/kg/d, respectively, and the kinetics followed a first-order process. No evidence of biphasic transport was found, in contrast to the situation in freshwater fish. Viscera were the most important sites of metal uptake, and gills were the second most important sites. The black sea breams were then acclimated at different Cd or Zn concentrations from either waterborne or dietary source for one week, and the alteration of metal uptake kinetics or subcellular distribution and metallothionein (MT) induction were further quantified. The Cd body burden was enhanced up to 8.6- and 49-fold after waterborne and dietary Cd pre-exposure, respectively. Cadmium pre-exposure also altered the tissue-specific subcellular Cd distribution and significantly elevated tissue MT levels. In contrast, the black sea breams were able to regulate Zn accumulation, and waterborne or dietary Zn pre-exposure had only weak influences on Zn body burden and redistribution. Both Cd and Zn pre-exposures enhanced the metal uptake rate constants, whereas the nonexchangeable surface bindings were less impacted by these pre-exposures. We demonstrated a positive relationship between the Cd uptake rate and Cd or MT concentration in the fish. Pre-exposure to metals may substantially modify the kinetics of metal uptake.",
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year = "2006",
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Alternative approaches can greatly reduce the number of fish used for acute toxicity testing. / Hoekzema, C.C.; Murk, A.J.; van de Waart, B.J.; van der Hoeven, J.C.M.; de Roode, D.F.

In: Environmental Toxicology and Chemistry, Vol. 25, No. 5, 2006, p. 1322-1325.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Alternative approaches can greatly reduce the number of fish used for acute toxicity testing

AU - Hoekzema, C.C.

AU - Murk, A.J.

AU - van de Waart, B.J.

AU - van der Hoeven, J.C.M.

AU - de Roode, D.F.

PY - 2006

Y1 - 2006

N2 - We first examined the uptake kinetics of Cd and Zn in the juvenile marine black sea bream (Acanthopagrus schlegeli) over a wide range of ambient Cd and Zn concentrations, and the relationships with metal accumulation (uptake rate and amount of nonexchangeable surface binding) were established for different fish tissues. Both Cd and Zn accumulation in the body increased linearly with exposure time after the initial metal surface binding. The dissolved Cd and Zn uptake rate constants were 2.64 and 6.50 L/kg/d, respectively, and the kinetics followed a first-order process. No evidence of biphasic transport was found, in contrast to the situation in freshwater fish. Viscera were the most important sites of metal uptake, and gills were the second most important sites. The black sea breams were then acclimated at different Cd or Zn concentrations from either waterborne or dietary source for one week, and the alteration of metal uptake kinetics or subcellular distribution and metallothionein (MT) induction were further quantified. The Cd body burden was enhanced up to 8.6- and 49-fold after waterborne and dietary Cd pre-exposure, respectively. Cadmium pre-exposure also altered the tissue-specific subcellular Cd distribution and significantly elevated tissue MT levels. In contrast, the black sea breams were able to regulate Zn accumulation, and waterborne or dietary Zn pre-exposure had only weak influences on Zn body burden and redistribution. Both Cd and Zn pre-exposures enhanced the metal uptake rate constants, whereas the nonexchangeable surface bindings were less impacted by these pre-exposures. We demonstrated a positive relationship between the Cd uptake rate and Cd or MT concentration in the fish. Pre-exposure to metals may substantially modify the kinetics of metal uptake.

AB - We first examined the uptake kinetics of Cd and Zn in the juvenile marine black sea bream (Acanthopagrus schlegeli) over a wide range of ambient Cd and Zn concentrations, and the relationships with metal accumulation (uptake rate and amount of nonexchangeable surface binding) were established for different fish tissues. Both Cd and Zn accumulation in the body increased linearly with exposure time after the initial metal surface binding. The dissolved Cd and Zn uptake rate constants were 2.64 and 6.50 L/kg/d, respectively, and the kinetics followed a first-order process. No evidence of biphasic transport was found, in contrast to the situation in freshwater fish. Viscera were the most important sites of metal uptake, and gills were the second most important sites. The black sea breams were then acclimated at different Cd or Zn concentrations from either waterborne or dietary source for one week, and the alteration of metal uptake kinetics or subcellular distribution and metallothionein (MT) induction were further quantified. The Cd body burden was enhanced up to 8.6- and 49-fold after waterborne and dietary Cd pre-exposure, respectively. Cadmium pre-exposure also altered the tissue-specific subcellular Cd distribution and significantly elevated tissue MT levels. In contrast, the black sea breams were able to regulate Zn accumulation, and waterborne or dietary Zn pre-exposure had only weak influences on Zn body burden and redistribution. Both Cd and Zn pre-exposures enhanced the metal uptake rate constants, whereas the nonexchangeable surface bindings were less impacted by these pre-exposures. We demonstrated a positive relationship between the Cd uptake rate and Cd or MT concentration in the fish. Pre-exposure to metals may substantially modify the kinetics of metal uptake.

KW - toxiciteit

KW - chemicaliën

KW - tests

KW - toxicologie

KW - testen

KW - vissen

KW - toxicity

KW - chemicals

KW - tests

KW - toxicology

KW - testing

KW - fishes

KW - european-union

KW - ecotoxicity

KW - strategy

KW - daphnia

KW - embryo

KW - algae

U2 - 10.1897/05-293R2.1

DO - 10.1897/05-293R2.1

M3 - Article

VL - 25

SP - 1322

EP - 1325

JO - Environmental Toxicology and Chemistry

T2 - Environmental Toxicology and Chemistry

JF - Environmental Toxicology and Chemistry

SN - 0730-7268

IS - 5

ER -