Altered Gut Microbial Fermentation and Colonization with Methanobrevibacter smithii in Renal Transplant Recipients

Tim J. Knobbe; Rianne M. Douwes; Daan Kremer; Casper J. Swarte; Michele F. Eisenga; António W. Gomes-Neto; Marco van Londen; Frans T.M. Peters; Hans Blokzijl; Ilja M. Nolte; Wouter H. Hendriks; Hermie J.M. Harmsen; Stephan J.L. Bakker

doi:10.3390/jcm9020518

Altered Gut Microbial Fermentation and Colonization with Methanobrevibacter smithii in Renal Transplant Recipients

Tim J. Knobbe^*, Rianne M. Douwes, Daan Kremer, Casper J. Swarte, Michele F. Eisenga, António W. Gomes-Neto, Marco van Londen, Frans T.M. Peters, Hans Blokzijl, Ilja M. Nolte, Wouter H. Hendriks, Hermie J.M. Harmsen, Stephan J.L. Bakker

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

7 Citations (Scopus)

Abstract

Renal transplant recipients (RTRs) often suffer from posttransplant diarrhea. The observed dysbiosis in RTR may influence the fermentation processes in the gut. In this study, we aimed to investigate whether fermentation differs between RTRs and healthy controls (HCs), by measuring breath H2 and CH4 concentrations. Additionally, we determined the fecal presence of the methanogen Methanobrevibacter smithii (M. smithii), which plays a main role in the process of methanogenesis. Data from the TransplantLines Biobank and Cohort Study (NCT03272841) was used. A total of 142 RTRs and 77 HCs were included. Breath H2 concentrations in RTRs were not significantly different from HCs. Breath CH4 concentrations in RTRs were significantly lower compared with HCs (median [interquartile range (IQR)] 7.5 [3.9–10.6] ppm vs. 16.0 [8.0–45.5] ppm, p < 0.001). M. smithii was less frequently present in the feces of RTRs compared to HCs (28.6% vs. 86.4% resp., p < 0.001). Our findings regarding the altered methanogenesis in the gut of RTRs show similarities with previous results in inflammatory bowel disease patients. These findings provide novel insight into the alterations of fermentation after renal transplantation, which may contribute to understanding the occurrence of posttransplant diarrhea. View Full-Text

Original language	English
Article number	518
Journal	Journal of Clinical Medicine
Volume	9
Issue number	2
DOIs	https://doi.org/10.3390/jcm9020518
Publication status	Published - 14 Feb 2020

Keywords

Methanogenesis
Methanosphaera stadtmanae
Mucins
Posttransplant diarrhea
Sulfate-reducing bacteria

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Knobbe, T. J., Douwes, R. M., Kremer, D., Swarte, C. J., Eisenga, M. F., Gomes-Neto, A. W., van Londen, M., Peters, F. T. M., Blokzijl, H., Nolte, I. M., Hendriks, W. H., Harmsen, H. J. M., & Bakker, S. J. L. (2020). Altered Gut Microbial Fermentation and Colonization with Methanobrevibacter smithii in Renal Transplant Recipients. Journal of Clinical Medicine, 9(2), Article 518. https://doi.org/10.3390/jcm9020518

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abstract = "Renal transplant recipients (RTRs) often suffer from posttransplant diarrhea. The observed dysbiosis in RTR may influence the fermentation processes in the gut. In this study, we aimed to investigate whether fermentation differs between RTRs and healthy controls (HCs), by measuring breath H2 and CH4 concentrations. Additionally, we determined the fecal presence of the methanogen Methanobrevibacter smithii (M. smithii), which plays a main role in the process of methanogenesis. Data from the TransplantLines Biobank and Cohort Study (NCT03272841) was used. A total of 142 RTRs and 77 HCs were included. Breath H2 concentrations in RTRs were not significantly different from HCs. Breath CH4 concentrations in RTRs were significantly lower compared with HCs (median [interquartile range (IQR)] 7.5 [3.9–10.6] ppm vs. 16.0 [8.0–45.5] ppm, p < 0.001). M. smithii was less frequently present in the feces of RTRs compared to HCs (28.6% vs. 86.4% resp., p < 0.001). Our findings regarding the altered methanogenesis in the gut of RTRs show similarities with previous results in inflammatory bowel disease patients. These findings provide novel insight into the alterations of fermentation after renal transplantation, which may contribute to understanding the occurrence of posttransplant diarrhea. View Full-Text ",

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Knobbe, TJ, Douwes, RM, Kremer, D, Swarte, CJ, Eisenga, MF, Gomes-Neto, AW, van Londen, M, Peters, FTM, Blokzijl, H, Nolte, IM, Hendriks, WH, Harmsen, HJM & Bakker, SJL 2020, 'Altered Gut Microbial Fermentation and Colonization with Methanobrevibacter smithii in Renal Transplant Recipients', Journal of Clinical Medicine, vol. 9, no. 2, 518. https://doi.org/10.3390/jcm9020518

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T1 - Altered Gut Microbial Fermentation and Colonization with Methanobrevibacter smithii in Renal Transplant Recipients

AU - Knobbe, Tim J.

AU - Douwes, Rianne M.

AU - Kremer, Daan

AU - Swarte, Casper J.

AU - Eisenga, Michele F.

AU - Gomes-Neto, António W.

AU - van Londen, Marco

AU - Peters, Frans T.M.

AU - Blokzijl, Hans

AU - Nolte, Ilja M.

AU - Hendriks, Wouter H.

AU - Harmsen, Hermie J.M.

AU - Bakker, Stephan J.L.

PY - 2020/2/14

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N2 - Renal transplant recipients (RTRs) often suffer from posttransplant diarrhea. The observed dysbiosis in RTR may influence the fermentation processes in the gut. In this study, we aimed to investigate whether fermentation differs between RTRs and healthy controls (HCs), by measuring breath H2 and CH4 concentrations. Additionally, we determined the fecal presence of the methanogen Methanobrevibacter smithii (M. smithii), which plays a main role in the process of methanogenesis. Data from the TransplantLines Biobank and Cohort Study (NCT03272841) was used. A total of 142 RTRs and 77 HCs were included. Breath H2 concentrations in RTRs were not significantly different from HCs. Breath CH4 concentrations in RTRs were significantly lower compared with HCs (median [interquartile range (IQR)] 7.5 [3.9–10.6] ppm vs. 16.0 [8.0–45.5] ppm, p < 0.001). M. smithii was less frequently present in the feces of RTRs compared to HCs (28.6% vs. 86.4% resp., p < 0.001). Our findings regarding the altered methanogenesis in the gut of RTRs show similarities with previous results in inflammatory bowel disease patients. These findings provide novel insight into the alterations of fermentation after renal transplantation, which may contribute to understanding the occurrence of posttransplant diarrhea. View Full-Text

AB - Renal transplant recipients (RTRs) often suffer from posttransplant diarrhea. The observed dysbiosis in RTR may influence the fermentation processes in the gut. In this study, we aimed to investigate whether fermentation differs between RTRs and healthy controls (HCs), by measuring breath H2 and CH4 concentrations. Additionally, we determined the fecal presence of the methanogen Methanobrevibacter smithii (M. smithii), which plays a main role in the process of methanogenesis. Data from the TransplantLines Biobank and Cohort Study (NCT03272841) was used. A total of 142 RTRs and 77 HCs were included. Breath H2 concentrations in RTRs were not significantly different from HCs. Breath CH4 concentrations in RTRs were significantly lower compared with HCs (median [interquartile range (IQR)] 7.5 [3.9–10.6] ppm vs. 16.0 [8.0–45.5] ppm, p < 0.001). M. smithii was less frequently present in the feces of RTRs compared to HCs (28.6% vs. 86.4% resp., p < 0.001). Our findings regarding the altered methanogenesis in the gut of RTRs show similarities with previous results in inflammatory bowel disease patients. These findings provide novel insight into the alterations of fermentation after renal transplantation, which may contribute to understanding the occurrence of posttransplant diarrhea. View Full-Text

KW - Methanogenesis

KW - Methanosphaera stadtmanae

KW - Mucins

KW - Posttransplant diarrhea

KW - Sulfate-reducing bacteria

U2 - 10.3390/jcm9020518

DO - 10.3390/jcm9020518

M3 - Article

SN - 2077-0383

VL - 9

JO - Journal of Clinical Medicine

JF - Journal of Clinical Medicine

IS - 2

M1 - 518

ER -

Altered Gut Microbial Fermentation and Colonization with Methanobrevibacter smithii in Renal Transplant Recipients

Abstract

Keywords

UN SDGs

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