Alteration of cell wall polysaccharides through transgenic expression of UDP-Glc 4-epimerase-encoding genes in potato tubers

Jie Hong Huang, Anne Kortstee, Dianka C.T. Dees, Luisa M. Trindade, Henk A. Schols, Harry Gruppen*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

Uridine diphosphate (UDP)-glucose 4-epimerase (UGE) catalyzes the conversion of UDP-glucose to UDP-galactose. Cell wall materials from the cv. Kardal (wild-type, background) and two UGE transgenic lines (UGE 45-1 and UGE 51-16) were isolated and fractionated. The galactose (Gal) content (mg/100 g tuber) from UGE 45-1 transgenic line was 38% higher than that of wild-type, and resulted in longer pectin side chains. The Gal content present in UGE 51-16 was 17% lower than that of wild-type, although most pectin populations maintained the same level of Gal. Both UGE transgenic lines showed unexpectedly a decrease in acetylation and an increase in methyl-esterification of pectin. Both UGE transgenic lines showed similar proportions of homogalacturonan and rhamnogalacturonan I within pectin backbone as the wild-type, except for the calcium-bound pectin fraction exhibiting relatively less rhamnogalacturonan I. Next to pectin modification, xyloglucan populations from both transgenic lines were altered resulting in different XSGG and XXGG proportion in comparison to wild-type.

Original languageEnglish
Pages (from-to)337-344
JournalCarbohydrate Polymers
Volume146
DOIs
Publication statusPublished - 2016

Fingerprint

UDPglucose 4-Epimerase
Racemases and Epimerases
Gene encoding
Uridine Diphosphate
Polysaccharides
Glucose
Cells
Galactose
Uridine Diphosphate Glucose
Uridine Diphosphate Galactose
Acetylation
Esterification
glucose tetrasaccharide
pectin
Calcium

Keywords

  • Cell wall polysaccharides
  • Nucleotide sugar interconversion
  • Plant biosynthesis
  • Solanum tuberosum
  • Transgenic potato
  • UDP-sugars

Cite this

@article{cf68d200eaaa4fbcbae2aa9a9f6d6265,
title = "Alteration of cell wall polysaccharides through transgenic expression of UDP-Glc 4-epimerase-encoding genes in potato tubers",
abstract = "Uridine diphosphate (UDP)-glucose 4-epimerase (UGE) catalyzes the conversion of UDP-glucose to UDP-galactose. Cell wall materials from the cv. Kardal (wild-type, background) and two UGE transgenic lines (UGE 45-1 and UGE 51-16) were isolated and fractionated. The galactose (Gal) content (mg/100 g tuber) from UGE 45-1 transgenic line was 38{\%} higher than that of wild-type, and resulted in longer pectin side chains. The Gal content present in UGE 51-16 was 17{\%} lower than that of wild-type, although most pectin populations maintained the same level of Gal. Both UGE transgenic lines showed unexpectedly a decrease in acetylation and an increase in methyl-esterification of pectin. Both UGE transgenic lines showed similar proportions of homogalacturonan and rhamnogalacturonan I within pectin backbone as the wild-type, except for the calcium-bound pectin fraction exhibiting relatively less rhamnogalacturonan I. Next to pectin modification, xyloglucan populations from both transgenic lines were altered resulting in different XSGG and XXGG proportion in comparison to wild-type.",
keywords = "Cell wall polysaccharides, Nucleotide sugar interconversion, Plant biosynthesis, Solanum tuberosum, Transgenic potato, UDP-sugars",
author = "Huang, {Jie Hong} and Anne Kortstee and Dees, {Dianka C.T.} and Trindade, {Luisa M.} and Schols, {Henk A.} and Harry Gruppen",
year = "2016",
doi = "10.1016/j.carbpol.2016.03.044",
language = "English",
volume = "146",
pages = "337--344",
journal = "Carbohydrate Polymers",
issn = "0144-8617",
publisher = "Elsevier",

}

Alteration of cell wall polysaccharides through transgenic expression of UDP-Glc 4-epimerase-encoding genes in potato tubers. / Huang, Jie Hong; Kortstee, Anne; Dees, Dianka C.T.; Trindade, Luisa M.; Schols, Henk A.; Gruppen, Harry.

In: Carbohydrate Polymers, Vol. 146, 2016, p. 337-344.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Alteration of cell wall polysaccharides through transgenic expression of UDP-Glc 4-epimerase-encoding genes in potato tubers

AU - Huang, Jie Hong

AU - Kortstee, Anne

AU - Dees, Dianka C.T.

AU - Trindade, Luisa M.

AU - Schols, Henk A.

AU - Gruppen, Harry

PY - 2016

Y1 - 2016

N2 - Uridine diphosphate (UDP)-glucose 4-epimerase (UGE) catalyzes the conversion of UDP-glucose to UDP-galactose. Cell wall materials from the cv. Kardal (wild-type, background) and two UGE transgenic lines (UGE 45-1 and UGE 51-16) were isolated and fractionated. The galactose (Gal) content (mg/100 g tuber) from UGE 45-1 transgenic line was 38% higher than that of wild-type, and resulted in longer pectin side chains. The Gal content present in UGE 51-16 was 17% lower than that of wild-type, although most pectin populations maintained the same level of Gal. Both UGE transgenic lines showed unexpectedly a decrease in acetylation and an increase in methyl-esterification of pectin. Both UGE transgenic lines showed similar proportions of homogalacturonan and rhamnogalacturonan I within pectin backbone as the wild-type, except for the calcium-bound pectin fraction exhibiting relatively less rhamnogalacturonan I. Next to pectin modification, xyloglucan populations from both transgenic lines were altered resulting in different XSGG and XXGG proportion in comparison to wild-type.

AB - Uridine diphosphate (UDP)-glucose 4-epimerase (UGE) catalyzes the conversion of UDP-glucose to UDP-galactose. Cell wall materials from the cv. Kardal (wild-type, background) and two UGE transgenic lines (UGE 45-1 and UGE 51-16) were isolated and fractionated. The galactose (Gal) content (mg/100 g tuber) from UGE 45-1 transgenic line was 38% higher than that of wild-type, and resulted in longer pectin side chains. The Gal content present in UGE 51-16 was 17% lower than that of wild-type, although most pectin populations maintained the same level of Gal. Both UGE transgenic lines showed unexpectedly a decrease in acetylation and an increase in methyl-esterification of pectin. Both UGE transgenic lines showed similar proportions of homogalacturonan and rhamnogalacturonan I within pectin backbone as the wild-type, except for the calcium-bound pectin fraction exhibiting relatively less rhamnogalacturonan I. Next to pectin modification, xyloglucan populations from both transgenic lines were altered resulting in different XSGG and XXGG proportion in comparison to wild-type.

KW - Cell wall polysaccharides

KW - Nucleotide sugar interconversion

KW - Plant biosynthesis

KW - Solanum tuberosum

KW - Transgenic potato

KW - UDP-sugars

U2 - 10.1016/j.carbpol.2016.03.044

DO - 10.1016/j.carbpol.2016.03.044

M3 - Article

VL - 146

SP - 337

EP - 344

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

SN - 0144-8617

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