Alpha-gliadin genes from the A, B, and D genomes of wheat contain different sets of celiac disease epitopes

T.W.J.M. van Herpen, V. Goryunova-Svetlana, J. van der Schoot, M. Mitreva, E.M.J. Salentijn, O.F.J. Vorst, M.F. Schenk, P. van Veelen, F. de Koning, L.J.M. van Soest, B.J. Vosman, H.J. Bosch, L.J.W.J. Gilissen, M.J.M. Smulders

Research output: Contribution to journalArticleAcademicpeer-review

191 Citations (Scopus)


Background - Bread wheat (Triticum aestivum) is an important staple food. However, wheat gluten proteins cause celiac disease (CD) in 0.5 to 1% of the general population. Among these proteins, the a-gliadins contain several peptides that are associated to the disease. Results - We obtained 230 distinct a-gliadin gene sequences from severaldiploid wheat species representing the ancestral A, B, and D genomes of the hexaploid bread wheat. The large majority of these sequences (87%) contained an internal stop codon. All a-gliadin sequences could be distinguished according to the genome of origin on the basis of sequence similarity, of the average length of the polyglutamine repeats, and of the differences in the presence of four peptides that have been identified as T cell stimulatory epitopes in CD patients through binding to HLA-DQ2/8. By sequence similarity, a-gliadins from the public database of hexaploid T. aestivum could be assigned directly to chromosome 6A, 6B, or 6D. T. monococcum (A genome) sequences, as well as those from chromosome 6A of bread wheat, almost invariably contained epitope glia-a9 and glia-a20, but never the intact epitopes glia-a and glia-a2. A number of sequences from T. speltoides, as well as a number of sequences fromchromosome 6B of bread wheat, did not contain any of the four T cell epitopes screened for. The sequences from T. tauschii (D genome), as well as those from chromosome 6D of bread wheat, were found to contain all of these T cell epitopes in variable combinations per gene. The differences in epitope composition resulted mainly from point mutations. These substitutions appeared to be genome specific. Conclusion - Our analysis shows that a-gliadin sequences from the three genomes of bread wheat form distinct groups. The four known T cell stimulatory epitopes are distributed non-randomly across the sequences, indicating that the three genomes contribute differently to epitope content. A systematic analysis of all known epitopes in gliadins and glutenins will lead to better understanding of the differences in toxicity among wheat varieties. On the basis of such insight, breeding strategies can be designed to generate less toxic varieties of wheat which may be tolerated by at least part of the CD patient population.
Original languageEnglish
Article number1
JournalBMC Genomics
Publication statusPublished - 2006


  • tissue transglutaminase
  • hexaploid wheat
  • cereal toxicity
  • t-cells
  • dna
  • evolution
  • peptides
  • family
  • loci


Dive into the research topics of 'Alpha-gliadin genes from the A, B, and D genomes of wheat contain different sets of celiac disease epitopes'. Together they form a unique fingerprint.

Cite this