Alkaline pretreatments of lignin-rich by-products and their implications for enzymatic degradation

P. Murciano-Martinez

Research output: Thesisinternal PhD, WU


The increasing interest in plant biomass based biofuels and chemicals arouses mainly from the increased awareness of a possible finiteness of fuels. The current main challenge to produce such biofuels and biochemicals is economic efficiency, but also knowledge concerning type and effectiveness of both thermally assisted chemical and enzymatic treatments, needed to generate fermentable sugars, is lacking. The subject of this thesis is to gain understanding of the effect of both sulphuric acid and NaOH catalysed pretreatments of sugar cane bagasse and oil palm empty fruit bunches on subsequent enzymatic saccharification, with a focus on the NaOH catalysed pretreatments. The fate of the main polymers present, lignin, cellulose and xylan, was studied and also single-activity xylan degrading enzymes, all from Rasamsonia emersonii, were studied for their mode-of-action.

Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
  • Gruppen, Harry, Promotor
  • Kabel, Mirjam, Co-promotor
Award date1 Apr 2016
Place of PublicationWageningen
Print ISBNs9789462576629
Publication statusPublished - 2016


  • degradation
  • enzymes
  • pretreatment
  • byproducts
  • lignin
  • food chemistry
  • delignification
  • sugarcane bagasse

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  • Projects

    Process development for the production of C3-acids from lignocellulosic feedstocks.

    Murciano Martinez, P., Gruppen, H. & Kabel, M.


    Project: PhD

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