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Abstract
Epidemic and pandemic outbreaks can greatly affect our societies, as observed with the recent SARS-CoV-2 pandemic. Consequently, it has become a priority to develop vaccination platforms rapidly adaptable to emerging threats. In this research, I make use of a novel tobacco-based cell-free protein synthesis system (known as ALiCE) to quickly develop, test, and produce vaccine candidates in the event of a pandemic. We show how the ALiCE system can be used to quickly screen different vaccine candidates, scale-up their production, and test their immunogenic properties. We also developed methods to support the purification of vaccine candidates produced at scale, and to allow higher-throughput screening approaches via the utilization of alternative genetic material. Lastly, a plug-and-play vaccination platform was developed that allows the rapid adaptation to different pathogens. Altogether, this research shows the potential of ALiCE to be used as a pandemic preparedness platform, which might be used in the future to quickly develop vaccine candidates upon an outbreak.
Original language | English |
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Qualification | Doctor of Philosophy |
Awarding Institution |
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Supervisors/Advisors |
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Award date | 21 Feb 2025 |
Place of Publication | Wageningen |
Publisher | |
DOIs | |
Publication status | Published - 21 Feb 2025 |
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Vaccine and therapeutic protein production in the ALiCE cell-free protein synthesis system
Armero Gimenez, J. (PhD candidate), Smant, G. (Promotor), Wilbers, R. (Co-promotor) & Schots, A. (Other)
1/11/20 → …
Project: PhD