Alcohol acetyl transferases for alkyl alkanoate production

A.J. Kruis (Inventor), M. Levisson (Inventor), A.E. Mars (Inventor), S.W.M. Kengen (Inventor), J. van der Oost (Inventor), J.P.M. Sanders (Inventor), R.A. Weusthuis (Inventor)

Research output: PatentOther research output

Abstract

The present invention is directed to a process for the production of alkyl alkanoate wherein a polypeptide that has alcohol acetyl transferase activity (AAT) and has in its three-dimensional structure an alpha-beta hydrolase fold and an active site comprising a serine, histidine, and optionally aspartic acid is, used to convert: - C3-C10 alkanol and acetyl coenzyme A or, - C1-C10 alkanol with C3-C10 acyl coenzyme A, or - Methanol with acetyl coenzyme A, into an alkyl alkanoate. The process is further directed to the use of a recombinant expression vector or plasmid or host cell comprising the nucleotide sequence encoding the polypeptide. The polypeptides according to the invention are genes isolated from Kluyveromyces marxianus, Kluyveromyces lactis, Wickerhamomyces ciferrii, Cyberlindnera jadinii, Hanseniaspora uvarum, Eremothecium cymbalarie and Saccharomyces cerevisiae.
Original languageEnglish
Patent numberWO2018099719
Priority date1/12/16
Publication statusPublished - 8 Jun 2018

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Transferases
Kluyveromyces
Acetyl Coenzyme A
Alcohols
Peptides
Eremothecium
Hanseniaspora
Acyl Coenzyme A
Hydrolases
Histidine
Aspartic Acid
Serine
Methanol
Saccharomyces cerevisiae
Catalytic Domain
Plasmids
Genes

Cite this

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title = "Alcohol acetyl transferases for alkyl alkanoate production",
abstract = "The present invention is directed to a process for the production of alkyl alkanoate wherein a polypeptide that has alcohol acetyl transferase activity (AAT) and has in its three-dimensional structure an alpha-beta hydrolase fold and an active site comprising a serine, histidine, and optionally aspartic acid is, used to convert: - C3-C10 alkanol and acetyl coenzyme A or, - C1-C10 alkanol with C3-C10 acyl coenzyme A, or - Methanol with acetyl coenzyme A, into an alkyl alkanoate. The process is further directed to the use of a recombinant expression vector or plasmid or host cell comprising the nucleotide sequence encoding the polypeptide. The polypeptides according to the invention are genes isolated from Kluyveromyces marxianus, Kluyveromyces lactis, Wickerhamomyces ciferrii, Cyberlindnera jadinii, Hanseniaspora uvarum, Eremothecium cymbalarie and Saccharomyces cerevisiae.",
author = "A.J. Kruis and M. Levisson and A.E. Mars and S.W.M. Kengen and {van der Oost}, J. and J.P.M. Sanders and R.A. Weusthuis",
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Alcohol acetyl transferases for alkyl alkanoate production. / Kruis, A.J. (Inventor); Levisson, M. (Inventor); Mars, A.E. (Inventor); Kengen, S.W.M. (Inventor); van der Oost, J. (Inventor); Sanders, J.P.M. (Inventor); Weusthuis, R.A. (Inventor).

Patent No.: WO2018099719.

Research output: PatentOther research output

TY - PAT

T1 - Alcohol acetyl transferases for alkyl alkanoate production

AU - Kruis, A.J.

AU - Levisson, M.

AU - Mars, A.E.

AU - Kengen, S.W.M.

AU - van der Oost, J.

AU - Sanders, J.P.M.

AU - Weusthuis, R.A.

PY - 2018/6/8

Y1 - 2018/6/8

N2 - The present invention is directed to a process for the production of alkyl alkanoate wherein a polypeptide that has alcohol acetyl transferase activity (AAT) and has in its three-dimensional structure an alpha-beta hydrolase fold and an active site comprising a serine, histidine, and optionally aspartic acid is, used to convert: - C3-C10 alkanol and acetyl coenzyme A or, - C1-C10 alkanol with C3-C10 acyl coenzyme A, or - Methanol with acetyl coenzyme A, into an alkyl alkanoate. The process is further directed to the use of a recombinant expression vector or plasmid or host cell comprising the nucleotide sequence encoding the polypeptide. The polypeptides according to the invention are genes isolated from Kluyveromyces marxianus, Kluyveromyces lactis, Wickerhamomyces ciferrii, Cyberlindnera jadinii, Hanseniaspora uvarum, Eremothecium cymbalarie and Saccharomyces cerevisiae.

AB - The present invention is directed to a process for the production of alkyl alkanoate wherein a polypeptide that has alcohol acetyl transferase activity (AAT) and has in its three-dimensional structure an alpha-beta hydrolase fold and an active site comprising a serine, histidine, and optionally aspartic acid is, used to convert: - C3-C10 alkanol and acetyl coenzyme A or, - C1-C10 alkanol with C3-C10 acyl coenzyme A, or - Methanol with acetyl coenzyme A, into an alkyl alkanoate. The process is further directed to the use of a recombinant expression vector or plasmid or host cell comprising the nucleotide sequence encoding the polypeptide. The polypeptides according to the invention are genes isolated from Kluyveromyces marxianus, Kluyveromyces lactis, Wickerhamomyces ciferrii, Cyberlindnera jadinii, Hanseniaspora uvarum, Eremothecium cymbalarie and Saccharomyces cerevisiae.

M3 - Patent

M1 - WO2018099719

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