Akkermansia muciniphila reduces Porphyromonas gingivalis-induced inflammation and periodontal bone destruction

Olivier Huck, Hannah Mulhall, George Rubin, Zev Kizelnik, Radha Iyer, John D. Perpich, Nasreen Haque, Patrice D. Cani, Willem M. de Vos, Salomon Amar*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

Aim: Akkermansia muciniphila is a beneficial gut commensal, whose anti-inflammatory properties have recently been demonstrated. This study aimed to evaluate the effect of A. muciniphila on Porphyromonas gingivalis elicited inflammation. Material and Methods: In lean and obese mice, A. muciniphila was administered in P. gingivalis-induced calvarial abscess and in experimental periodontitis model (EIP). Bone destruction and inflammation were evaluated by histomorphometric analysis. In vitro, A. muciniphila was co-cultured with P. gingivalis, growth and virulence factor expression was evaluated. Bone marrow macrophages (BMMϕ) and gingival epithelial cells (TIGK) were exposed to both bacterial strains, and the expression of inflammatory mediators, as well as tight junction markers, was analysed. Results: In a model of calvarial infection, A. muciniphila decreased inflammatory cell infiltration and bone destruction. In EIP, treatment with A. muciniphila resulted in a decreased alveolar bone loss. In vitro, the addition of A. muciniphila to P. gingivalis-infected BMMϕ increased anti-inflammatory IL-10 and decreased IL-12. Additionally, A. muciniphila exposure increases the expression of junctional integrity markers such as integrin-β1, E-cadherin and ZO-1 in TIGK cells. A. muciniphila co-culture with P. gingivalis reduced gingipains mRNA expression. Discussion: This study demonstrated the protective effects of A. muciniphila administration and may open consideration to its use as an adjunctive therapeutic agent to periodontal treatment.

Original languageEnglish
Pages (from-to)202-212
JournalJournal of Clinical Periodontology
Volume47
Issue number2
Early online date1 Nov 2019
DOIs
Publication statusPublished - Feb 2020

Keywords

  • infection
  • inflammation
  • periodontitis
  • probiotic

Fingerprint Dive into the research topics of 'Akkermansia muciniphila reduces Porphyromonas gingivalis-induced inflammation and periodontal bone destruction'. Together they form a unique fingerprint.

  • Cite this

    Huck, O., Mulhall, H., Rubin, G., Kizelnik, Z., Iyer, R., Perpich, J. D., Haque, N., Cani, P. D., de Vos, W. M., & Amar, S. (2020). Akkermansia muciniphila reduces Porphyromonas gingivalis-induced inflammation and periodontal bone destruction. Journal of Clinical Periodontology, 47(2), 202-212. https://doi.org/10.1111/jcpe.13214