Akkermansia muciniphila Exerts Lipid-Lowering and Immunomodulatory Effects without Affecting Neointima Formation in Hyperlipidemic APOE*3-Leiden.CETP Mice

Saeed Katiraei, Margreet R. de Vries, Alice H. Costain, Kathrin Thiem, Lisa R. Hoving, Janna A. van Diepen, Hermelijn H. Smits, Kristien E. Bouter, Patrick C.N. Rensen, Paul H.A. Quax, Max Nieuwdorp, Mihai G. Netea, Willem M. de Vos, Patrice D. Cani, Clara Belzer, Ko Willems van Dijk*, Jimmy F.P. Berbée, Vanessa van Harmelen

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Scope: Akkermansia muciniphila (A. muciniphila) is an intestinal commensal with anti-inflammatory properties both in the intestine and other organs. The aim is to investigate the effects of oral administration of A. muciniphila on lipid metabolism, immunity, and cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L).CETP mice. Methods and results: Hyperlipidemic male E3L.CETP mice are daily treated with 2 × 108 CFU A. muciniphila by oral gavage for 4 weeks and the effects are determined on plasma lipid levels, immune parameters, and cuff-induced neointima formation and composition. A. muciniphila administration lowers body weight and plasma total cholesterol and triglycerides levels. A. muciniphila influences the immune cell composition in mesenteric lymph nodes, as evident from an increased total B cell population, while reducing the total T cell and neutrophil populations. Importantly, A. muciniphila reduces the expression of the activation markers MHCII on dendritic cells and CD86 on B cells. A. muciniphila also increases whole blood ex vivo lipopolysaccharide-stimulated IL-10 release. Finally, although treatment with A. muciniphila improves lipid metabolism and immunity, it does not affect neointima formation or composition. Conclusions: Four weeks of treatment with A. muciniphila exerts lipid-lowering and immunomodulatory effects, which are insufficient to inhibit neointima formation in hyperlipidemic E3L.CETP mice.

Original languageEnglish
Article number1900732
JournalMolecular Nutrition and Food Research
DOIs
Publication statusE-pub ahead of print - 6 Aug 2019

Fingerprint

Neointima
Lipids
lipid metabolism
B-lymphocytes
mice
lipids
immunity
Lipid Metabolism
Immunity
B-Lymphocytes
dendritic cells
interleukin-10
anti-inflammatory activity
blood lipids
lipopolysaccharides
oral administration
lymph nodes
neutrophils
mouth
intestines

Keywords

  • Akkermansia muciniphila
  • atherosclerosis
  • immunity
  • lipid metabolism
  • mesenteric lymph nodes

Cite this

Katiraei, Saeed ; de Vries, Margreet R. ; Costain, Alice H. ; Thiem, Kathrin ; Hoving, Lisa R. ; van Diepen, Janna A. ; Smits, Hermelijn H. ; Bouter, Kristien E. ; Rensen, Patrick C.N. ; Quax, Paul H.A. ; Nieuwdorp, Max ; Netea, Mihai G. ; de Vos, Willem M. ; Cani, Patrice D. ; Belzer, Clara ; van Dijk, Ko Willems ; Berbée, Jimmy F.P. ; van Harmelen, Vanessa. / Akkermansia muciniphila Exerts Lipid-Lowering and Immunomodulatory Effects without Affecting Neointima Formation in Hyperlipidemic APOE*3-Leiden.CETP Mice. In: Molecular Nutrition and Food Research. 2019.
@article{db6a674dbf3d4bcdba9aafd3af3dcee7,
title = "Akkermansia muciniphila Exerts Lipid-Lowering and Immunomodulatory Effects without Affecting Neointima Formation in Hyperlipidemic APOE*3-Leiden.CETP Mice",
abstract = "Scope: Akkermansia muciniphila (A. muciniphila) is an intestinal commensal with anti-inflammatory properties both in the intestine and other organs. The aim is to investigate the effects of oral administration of A. muciniphila on lipid metabolism, immunity, and cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L).CETP mice. Methods and results: Hyperlipidemic male E3L.CETP mice are daily treated with 2 × 108 CFU A. muciniphila by oral gavage for 4 weeks and the effects are determined on plasma lipid levels, immune parameters, and cuff-induced neointima formation and composition. A. muciniphila administration lowers body weight and plasma total cholesterol and triglycerides levels. A. muciniphila influences the immune cell composition in mesenteric lymph nodes, as evident from an increased total B cell population, while reducing the total T cell and neutrophil populations. Importantly, A. muciniphila reduces the expression of the activation markers MHCII on dendritic cells and CD86 on B cells. A. muciniphila also increases whole blood ex vivo lipopolysaccharide-stimulated IL-10 release. Finally, although treatment with A. muciniphila improves lipid metabolism and immunity, it does not affect neointima formation or composition. Conclusions: Four weeks of treatment with A. muciniphila exerts lipid-lowering and immunomodulatory effects, which are insufficient to inhibit neointima formation in hyperlipidemic E3L.CETP mice.",
keywords = "Akkermansia muciniphila, atherosclerosis, immunity, lipid metabolism, mesenteric lymph nodes",
author = "Saeed Katiraei and {de Vries}, {Margreet R.} and Costain, {Alice H.} and Kathrin Thiem and Hoving, {Lisa R.} and {van Diepen}, {Janna A.} and Smits, {Hermelijn H.} and Bouter, {Kristien E.} and Rensen, {Patrick C.N.} and Quax, {Paul H.A.} and Max Nieuwdorp and Netea, {Mihai G.} and {de Vos}, {Willem M.} and Cani, {Patrice D.} and Clara Belzer and {van Dijk}, {Ko Willems} and Berb{\'e}e, {Jimmy F.P.} and {van Harmelen}, Vanessa",
year = "2019",
month = "8",
day = "6",
doi = "10.1002/mnfr.201900732",
language = "English",
journal = "Molecular Nutrition & Food Research",
issn = "1613-4125",
publisher = "Wiley-VCH Verlag",

}

Katiraei, S, de Vries, MR, Costain, AH, Thiem, K, Hoving, LR, van Diepen, JA, Smits, HH, Bouter, KE, Rensen, PCN, Quax, PHA, Nieuwdorp, M, Netea, MG, de Vos, WM, Cani, PD, Belzer, C, van Dijk, KW, Berbée, JFP & van Harmelen, V 2019, 'Akkermansia muciniphila Exerts Lipid-Lowering and Immunomodulatory Effects without Affecting Neointima Formation in Hyperlipidemic APOE*3-Leiden.CETP Mice', Molecular Nutrition and Food Research. https://doi.org/10.1002/mnfr.201900732

Akkermansia muciniphila Exerts Lipid-Lowering and Immunomodulatory Effects without Affecting Neointima Formation in Hyperlipidemic APOE*3-Leiden.CETP Mice. / Katiraei, Saeed; de Vries, Margreet R.; Costain, Alice H.; Thiem, Kathrin; Hoving, Lisa R.; van Diepen, Janna A.; Smits, Hermelijn H.; Bouter, Kristien E.; Rensen, Patrick C.N.; Quax, Paul H.A.; Nieuwdorp, Max; Netea, Mihai G.; de Vos, Willem M.; Cani, Patrice D.; Belzer, Clara; van Dijk, Ko Willems; Berbée, Jimmy F.P.; van Harmelen, Vanessa.

In: Molecular Nutrition and Food Research, 06.08.2019.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Akkermansia muciniphila Exerts Lipid-Lowering and Immunomodulatory Effects without Affecting Neointima Formation in Hyperlipidemic APOE*3-Leiden.CETP Mice

AU - Katiraei, Saeed

AU - de Vries, Margreet R.

AU - Costain, Alice H.

AU - Thiem, Kathrin

AU - Hoving, Lisa R.

AU - van Diepen, Janna A.

AU - Smits, Hermelijn H.

AU - Bouter, Kristien E.

AU - Rensen, Patrick C.N.

AU - Quax, Paul H.A.

AU - Nieuwdorp, Max

AU - Netea, Mihai G.

AU - de Vos, Willem M.

AU - Cani, Patrice D.

AU - Belzer, Clara

AU - van Dijk, Ko Willems

AU - Berbée, Jimmy F.P.

AU - van Harmelen, Vanessa

PY - 2019/8/6

Y1 - 2019/8/6

N2 - Scope: Akkermansia muciniphila (A. muciniphila) is an intestinal commensal with anti-inflammatory properties both in the intestine and other organs. The aim is to investigate the effects of oral administration of A. muciniphila on lipid metabolism, immunity, and cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L).CETP mice. Methods and results: Hyperlipidemic male E3L.CETP mice are daily treated with 2 × 108 CFU A. muciniphila by oral gavage for 4 weeks and the effects are determined on plasma lipid levels, immune parameters, and cuff-induced neointima formation and composition. A. muciniphila administration lowers body weight and plasma total cholesterol and triglycerides levels. A. muciniphila influences the immune cell composition in mesenteric lymph nodes, as evident from an increased total B cell population, while reducing the total T cell and neutrophil populations. Importantly, A. muciniphila reduces the expression of the activation markers MHCII on dendritic cells and CD86 on B cells. A. muciniphila also increases whole blood ex vivo lipopolysaccharide-stimulated IL-10 release. Finally, although treatment with A. muciniphila improves lipid metabolism and immunity, it does not affect neointima formation or composition. Conclusions: Four weeks of treatment with A. muciniphila exerts lipid-lowering and immunomodulatory effects, which are insufficient to inhibit neointima formation in hyperlipidemic E3L.CETP mice.

AB - Scope: Akkermansia muciniphila (A. muciniphila) is an intestinal commensal with anti-inflammatory properties both in the intestine and other organs. The aim is to investigate the effects of oral administration of A. muciniphila on lipid metabolism, immunity, and cuff-induced neointima formation in hyperlipidemic APOE*3-Leiden (E3L).CETP mice. Methods and results: Hyperlipidemic male E3L.CETP mice are daily treated with 2 × 108 CFU A. muciniphila by oral gavage for 4 weeks and the effects are determined on plasma lipid levels, immune parameters, and cuff-induced neointima formation and composition. A. muciniphila administration lowers body weight and plasma total cholesterol and triglycerides levels. A. muciniphila influences the immune cell composition in mesenteric lymph nodes, as evident from an increased total B cell population, while reducing the total T cell and neutrophil populations. Importantly, A. muciniphila reduces the expression of the activation markers MHCII on dendritic cells and CD86 on B cells. A. muciniphila also increases whole blood ex vivo lipopolysaccharide-stimulated IL-10 release. Finally, although treatment with A. muciniphila improves lipid metabolism and immunity, it does not affect neointima formation or composition. Conclusions: Four weeks of treatment with A. muciniphila exerts lipid-lowering and immunomodulatory effects, which are insufficient to inhibit neointima formation in hyperlipidemic E3L.CETP mice.

KW - Akkermansia muciniphila

KW - atherosclerosis

KW - immunity

KW - lipid metabolism

KW - mesenteric lymph nodes

U2 - 10.1002/mnfr.201900732

DO - 10.1002/mnfr.201900732

M3 - Article

JO - Molecular Nutrition & Food Research

JF - Molecular Nutrition & Food Research

SN - 1613-4125

M1 - 1900732

ER -