Airborne microorganisms and dust from livestock houses

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The objective of this study was to evaluate the efficiencies and suitability of samplers for airborne microorganisms and dust, which could be used in practical livestock houses. Two studies were performed: 1) Testing impaction and cyclone pre-separators for dust sampling in livestock houses; 2) Determining sampling efficiencies of four bioaerosol samplers for bacteria and virus. Study 1. The overloading problem of the EU reference impaction pre-separator (IPS) was tested in layer houses and compared with cyclone pre-separators (CPS) for sampling PM10 and PM2.5. Study 2. Physical and biological efficiencies of Andersen 6-stage impactor, all glass impinger (AGI-30), high air flow rate sampler OMNI-3000, and MD8 with gelatin filter were investigated for collecting aerosolized bacteria, Enterococcus faecalis, Escherichia coli, Campylobacter jejuni and Mycoplasma synoviae and live Gumboro vaccine virus. A tracer (uranine) was used to determine physical efficiencies and bioaerosol deposition. The study was done in a HEPA isolator (volume: 1.3 m3). The results show the PM10 IPS did not become overloaded in 24 h measurements in layer houses, whereas PM2.5 IPS became overloaded within 1 h. CPS did not become overloaded during 48 h sampling of both dust fractions. The OMNI-3000 (62%) had lower physical efficiency than the MD8, while the other samplers had similar efficiencies as MD8. All the bioaerosol samplers had high biological efficiencies for all four bacterial species, except for C. jejuni (1%) when measured with the OMNI-3000 and for E. coli (38%) and C. jejuni (2%) when measured with the MD8. The biological efficiencies of the Andersen impactor (61%), the AGI-30 (90%) and the MD8 (163%) were not significantly different from 100% for collecting the aerosolized virus. However, the biological efficiency (23%) of the OMNI-3000 was significantly lower than 100%.
Original languageEnglish
Publication statusPublished - 2011
EventXVth International Congress on Animal Hygiene, Vienna, Austria -
Duration: 3 Jul 20117 Jul 2011


ConferenceXVth International Congress on Animal Hygiene, Vienna, Austria


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