AIL and HDG proteins act antagonistically to control cell proliferation

A. Horstman, H. Fukuoka, J.M. Muino Acuna, L.M.C. Nitsch, Changhao Guo, P.A. Passarinho, G.F. Sanchez Perez, R.G.H. Immink, G.C. Angenent, K.A. Boutilier

Research output: Contribution to journalArticleAcademicpeer-review

15 Citations (Scopus)

Abstract

AINTEGUMENTA-LIKE (AIL) transcription factors are key regulators of cell proliferation and meristem identity. Although AIL functions have been well described, the direct signalling components of this pathway are largely unknown.We show that BABY BOOM(BBM) and other AIL proteins physically interact with multiple members of the L1-expressed HOMEODOMAIN GLABROUS (HDG) transcription factor family, including HDG1, HDG11 and HDG12. Overexpression of HDG1, HDG11 and HDG12 restricts growth due to root and shoot meristem arrest, which is associated with reduced expression of genes involved in meristem development and cell proliferation pathways, whereas downregulation of multiple HDG genes promotes cell overproliferation. These results suggest a role for HDG proteins in promoting cell differentiation. We also reveal a transcriptional network in which BBM andHDG1regulate several common target genes, and whereBBM/AIL and HDG regulate the expression of each other. Taken together, these results suggest opposite roles for AIL and HDG proteins, with AILs promoting cell proliferation and HDGs stimulating cell differentiation, and that these functions are mediated at both the protein-protein interaction and transcriptional level.
Original languageEnglish
Pages (from-to)454-464
JournalDevelopment
Volume142
DOIs
Publication statusPublished - 2015

Keywords

  • arabidopsis-thaliana
  • transcription factors
  • plant transformation
  • ectopic expression
  • quantitative pcr
  • chip-seq
  • differentiation
  • genes
  • plethora
  • growth

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  • Datasets

    BABY BOOM (BBM) ChIP-seq in Arabidopsis somatic embryo tissue

    Horstman, A. (Creator), Muino Acuna, J. M. (Creator), Boutilier, K. (Creator), Plant Research International, 17 Dec 2014

    Dataset

    HDG1 transcription factor targets

    Horstman, A. (Creator), Boutilier, K. (Creator), Sanchez Perez, G. (Creator), Wageningen University, 22 Jan 2015

    Dataset

    Projects

    Som. embryogenese TTI-GG (KB-17-003.02-003)

    Boutilier, K.

    1/01/1131/12/15

    Project: EZproject

    Cite this