Agrobacterium-mediated Genetic Transformation of Chrysanthemum (Chrysanthemum morifolium Ramat.) with an Aphidicidal Gene, gcs (Gamma-cadinene Synthase)

Mahmood Valizadeh, S.K. Kazemitabar, M.A. Jongsma

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Florist’s chrysanthemum (Chrysanthemum morifolium Ramat.) belongs to the Asteraceae family and represents the second most important floricultural crop in the world. Unfortunately most genotypes are sensitive to aphids. Internode explants of 1581 and 4043 genotypes were incubated with A. tumefaciens strain AGL-0 containing pBIN plasmid with the npt gene as a selectable marker for kanamycin resistance and gcs gene as an aphicidal gene with rbcS promoter. Kanamycin resistant shoots were induced from internodes after 3 weeks. Finally, the shoots were rooted on MS medium containing 30 mg L-1 kanamycin. Incorporation and expression of the transgenes were confirmed by PCR and RT-PCR analysis. Genotype 4043 has been transformed in this study for the first time. Transformation frequency for GCS was 6.25 and 5% for genotypes 1581 and 4043, respectively.
Original languageEnglish
Pages (from-to)161-181
JournalInternational Journal of Plant Breeding and Genetics
Volume6
Issue number4
DOIs
Publication statusPublished - 2012

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Chrysanthemum morifolium
Chrysanthemum
genetic transformation
Agrobacterium
kanamycin
genotype
internodes
genes
floriculture crops
shoots
transgenes
Asteraceae
Aphidoidea
plasmids
explants
reverse transcriptase polymerase chain reaction
promoter regions

Cite this

@article{65fe1df286d84ba19e557e29ac17809c,
title = "Agrobacterium-mediated Genetic Transformation of Chrysanthemum (Chrysanthemum morifolium Ramat.) with an Aphidicidal Gene, gcs (Gamma-cadinene Synthase)",
abstract = "Florist’s chrysanthemum (Chrysanthemum morifolium Ramat.) belongs to the Asteraceae family and represents the second most important floricultural crop in the world. Unfortunately most genotypes are sensitive to aphids. Internode explants of 1581 and 4043 genotypes were incubated with A. tumefaciens strain AGL-0 containing pBIN plasmid with the npt gene as a selectable marker for kanamycin resistance and gcs gene as an aphicidal gene with rbcS promoter. Kanamycin resistant shoots were induced from internodes after 3 weeks. Finally, the shoots were rooted on MS medium containing 30 mg L-1 kanamycin. Incorporation and expression of the transgenes were confirmed by PCR and RT-PCR analysis. Genotype 4043 has been transformed in this study for the first time. Transformation frequency for GCS was 6.25 and 5{\%} for genotypes 1581 and 4043, respectively.",
author = "Mahmood Valizadeh and S.K. Kazemitabar and M.A. Jongsma",
year = "2012",
doi = "10.3923/ijpbg.2012.168.181",
language = "English",
volume = "6",
pages = "161--181",
journal = "International Journal of Plant Breeding and Genetics",
issn = "1819-3347",
publisher = "Science Alert",
number = "4",

}

Agrobacterium-mediated Genetic Transformation of Chrysanthemum (Chrysanthemum morifolium Ramat.) with an Aphidicidal Gene, gcs (Gamma-cadinene Synthase). / Valizadeh, Mahmood; Kazemitabar, S.K.; Jongsma, M.A.

In: International Journal of Plant Breeding and Genetics, Vol. 6, No. 4, 2012, p. 161-181.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Agrobacterium-mediated Genetic Transformation of Chrysanthemum (Chrysanthemum morifolium Ramat.) with an Aphidicidal Gene, gcs (Gamma-cadinene Synthase)

AU - Valizadeh, Mahmood

AU - Kazemitabar, S.K.

AU - Jongsma, M.A.

PY - 2012

Y1 - 2012

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AB - Florist’s chrysanthemum (Chrysanthemum morifolium Ramat.) belongs to the Asteraceae family and represents the second most important floricultural crop in the world. Unfortunately most genotypes are sensitive to aphids. Internode explants of 1581 and 4043 genotypes were incubated with A. tumefaciens strain AGL-0 containing pBIN plasmid with the npt gene as a selectable marker for kanamycin resistance and gcs gene as an aphicidal gene with rbcS promoter. Kanamycin resistant shoots were induced from internodes after 3 weeks. Finally, the shoots were rooted on MS medium containing 30 mg L-1 kanamycin. Incorporation and expression of the transgenes were confirmed by PCR and RT-PCR analysis. Genotype 4043 has been transformed in this study for the first time. Transformation frequency for GCS was 6.25 and 5% for genotypes 1581 and 4043, respectively.

U2 - 10.3923/ijpbg.2012.168.181

DO - 10.3923/ijpbg.2012.168.181

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SN - 1819-3347

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