Agaricus bisporus and Agaricus brasiliensis (1 ¿ 6)-ß-d-glucans show immunostimulatory activity on human THP-1 derived macrophages

F.R. Smiderle, G. Alquini, M.Z. Tadra-Sfeir, M. Lacomini, H.J. Wichers, L.J.L.D. van Griensven

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63 Citations (Scopus)

Abstract

The (1 ¿ 6)-ß-d-glucans from Agaricus bisporus and Agaricus brasiliensis were purified to evaluate their effects on the innate immune system. THP-1 macrophages were used to investigate the induction of the expression of TNF-a, IL1ß, and COX-2 by RT-PCR. The purification of the polysaccharides gave rise to fractions containing 96–98% of glucose. The samples were analyzed by GC–MS, HPSEC and 13C NMR, which confirmed the presence of homogeneous (1 ¿ 6)-ß-d-glucans. The ß-glucans were incubated with THP-1 derived macrophages, for 3 h and 6 h to evaluate their effects on the expression of pro-inflammatory genes. Both ß-glucans stimulated the expression of such genes as much as the pro-inflammatory control (LPS). When the cells were incubated with LPS + ß-glucan, a significant inhibition of the expression of IL-1ß and COX-2 was observed for both treatments after 3 h of incubation. By the results, we conclude that the (1 ¿ 6)-ß-d-glucans present an immunostimulatory activity when administered to THP-1 derived macrophages
Original languageEnglish
Pages (from-to)91-99
JournalCarbohydrate Polymers
Volume94
Issue number1
DOIs
Publication statusPublished - 2013

Keywords

  • beta-glucans
  • fungal metabolites
  • molecular-weight
  • polysaccharides
  • lipopolysaccharide
  • mushroom
  • activation
  • receptor
  • rats
  • expression

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