Adventitious root formation in Arabidopsis: underlying mechanisms and applications

Mehdi Massoumi

Research output: Thesisinternal PhD, WUAcademic

Abstract

Adventitious root (AR) formation is indispensable in vegetative propagation and is widely used. A better understanding of the underlying mechanisms is needed to improve rooting treatments. We first established a system to study rooting in Arabidopsis, the model organism in plant biology but only occasionally used to study adventitious rooting. Inhibition of polar auxin transport reduced AR formation. The role of auxin transporter proteins (several PIN-proteins) was found to be tissue-specific. Maturation (the transition from juvenile to adult) negatively influenced AR formation. Maturation was associated with increased DNA methylation and decreased miR156 level. 5-Azacytidine, a drug that reduces DNA methylation, increased rooting. We also examined the effect of two donor plant pre-treatments, etiolation and flooding, on rooting. Both increased AR formation.

Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
Supervisors/Advisors
  • Visser, Richard, Promotor
  • de Klerk, Geert-Jan, Co-promotor
  • Krens, Frans, Co-promotor
Award date6 Sep 2016
Place of PublicationWageningen
Publisher
Electronic ISBNs9789462578524
DOIs
Publication statusPublished - 2016

Fingerprint

adventitious roots
rooting
Arabidopsis
DNA methylation
auxins
etiolation
vegetative propagation
plant biology
transporters
proteins
pretreatment
drugs
organisms

Keywords

  • arabidopsis thaliana
  • adventitious roots
  • formation
  • plant development
  • quantitative traits
  • etiolation
  • auxins
  • explants
  • molecular biology
  • gene expression
  • dna methylation
  • rooting
  • ontogeny
  • plant breeding

Cite this

Massoumi, Mehdi. / Adventitious root formation in Arabidopsis : underlying mechanisms and applications. Wageningen : Wageningen University, 2016. 191 p.
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title = "Adventitious root formation in Arabidopsis: underlying mechanisms and applications",
abstract = "Adventitious root (AR) formation is indispensable in vegetative propagation and is widely used. A better understanding of the underlying mechanisms is needed to improve rooting treatments. We first established a system to study rooting in Arabidopsis, the model organism in plant biology but only occasionally used to study adventitious rooting. Inhibition of polar auxin transport reduced AR formation. The role of auxin transporter proteins (several PIN-proteins) was found to be tissue-specific. Maturation (the transition from juvenile to adult) negatively influenced AR formation. Maturation was associated with increased DNA methylation and decreased miR156 level. 5-Azacytidine, a drug that reduces DNA methylation, increased rooting. We also examined the effect of two donor plant pre-treatments, etiolation and flooding, on rooting. Both increased AR formation.",
keywords = "arabidopsis thaliana, adventitious roots, formation, plant development, quantitative traits, etiolation, auxins, explants, molecular biology, gene expression, dna methylation, rooting, ontogeny, plant breeding, arabidopsis thaliana, adventiefwortels, formatie, plantenontwikkeling, kwantitatieve kenmerken, etiolering, auxinen, explantaten, moleculaire biologie, genexpressie, dna-methylering, beworteling, ontogenie, plantenveredeling",
author = "Mehdi Massoumi",
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Massoumi, M 2016, 'Adventitious root formation in Arabidopsis: underlying mechanisms and applications', Doctor of Philosophy, Wageningen University, Wageningen. https://doi.org/10.18174/385310

Adventitious root formation in Arabidopsis : underlying mechanisms and applications. / Massoumi, Mehdi.

Wageningen : Wageningen University, 2016. 191 p.

Research output: Thesisinternal PhD, WUAcademic

TY - THES

T1 - Adventitious root formation in Arabidopsis

T2 - underlying mechanisms and applications

AU - Massoumi, Mehdi

N1 - WU thesis 6434 Includes bibliographic references. - With summary in English

PY - 2016

Y1 - 2016

N2 - Adventitious root (AR) formation is indispensable in vegetative propagation and is widely used. A better understanding of the underlying mechanisms is needed to improve rooting treatments. We first established a system to study rooting in Arabidopsis, the model organism in plant biology but only occasionally used to study adventitious rooting. Inhibition of polar auxin transport reduced AR formation. The role of auxin transporter proteins (several PIN-proteins) was found to be tissue-specific. Maturation (the transition from juvenile to adult) negatively influenced AR formation. Maturation was associated with increased DNA methylation and decreased miR156 level. 5-Azacytidine, a drug that reduces DNA methylation, increased rooting. We also examined the effect of two donor plant pre-treatments, etiolation and flooding, on rooting. Both increased AR formation.

AB - Adventitious root (AR) formation is indispensable in vegetative propagation and is widely used. A better understanding of the underlying mechanisms is needed to improve rooting treatments. We first established a system to study rooting in Arabidopsis, the model organism in plant biology but only occasionally used to study adventitious rooting. Inhibition of polar auxin transport reduced AR formation. The role of auxin transporter proteins (several PIN-proteins) was found to be tissue-specific. Maturation (the transition from juvenile to adult) negatively influenced AR formation. Maturation was associated with increased DNA methylation and decreased miR156 level. 5-Azacytidine, a drug that reduces DNA methylation, increased rooting. We also examined the effect of two donor plant pre-treatments, etiolation and flooding, on rooting. Both increased AR formation.

KW - arabidopsis thaliana

KW - adventitious roots

KW - formation

KW - plant development

KW - quantitative traits

KW - etiolation

KW - auxins

KW - explants

KW - molecular biology

KW - gene expression

KW - dna methylation

KW - rooting

KW - ontogeny

KW - plant breeding

KW - arabidopsis thaliana

KW - adventiefwortels

KW - formatie

KW - plantenontwikkeling

KW - kwantitatieve kenmerken

KW - etiolering

KW - auxinen

KW - explantaten

KW - moleculaire biologie

KW - genexpressie

KW - dna-methylering

KW - beworteling

KW - ontogenie

KW - plantenveredeling

U2 - 10.18174/385310

DO - 10.18174/385310

M3 - internal PhD, WU

PB - Wageningen University

CY - Wageningen

ER -