Abstract
Adventitious root (AR) formation is indispensable in vegetative propagation and is widely used. A better understanding of the underlying mechanisms is needed to improve rooting treatments. We first established a system to study rooting in Arabidopsis, the model organism in plant biology but only occasionally used to study adventitious rooting. Inhibition of polar auxin transport reduced AR formation. The role of auxin transporter proteins (several PIN-proteins) was found to be tissue-specific. Maturation (the transition from juvenile to adult) negatively influenced AR formation. Maturation was associated with increased DNA methylation and decreased miR156 level. 5-Azacytidine, a drug that reduces DNA methylation, increased rooting. We also examined the effect of two donor plant pre-treatments, etiolation and flooding, on rooting. Both increased AR formation.
| Original language | English |
|---|---|
| Qualification | Doctor of Philosophy |
| Awarding Institution |
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| Supervisors/Advisors |
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| Award date | 6 Sep 2016 |
| Place of Publication | Wageningen |
| Publisher | |
| Electronic ISBNs | 9789462578524 |
| DOIs | |
| Publication status | Published - 2016 |
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Keywords
- arabidopsis thaliana
- adventitious roots
- formation
- plant development
- quantitative traits
- etiolation
- auxins
- explants
- molecular biology
- gene expression
- dna methylation
- rooting
- ontogeny
- plant breeding
Cite this
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Adventitious root formation in Arabidopsis : underlying mechanisms and applications. / Massoumi, Mehdi.
Wageningen : Wageningen University, 2016. 191 p.Research output: Thesis › internal PhD, WU
TY - THES
T1 - Adventitious root formation in Arabidopsis
T2 - underlying mechanisms and applications
AU - Massoumi, Mehdi
N1 - WU thesis 6434 Includes bibliographic references. - With summary in English
PY - 2016
Y1 - 2016
N2 - Adventitious root (AR) formation is indispensable in vegetative propagation and is widely used. A better understanding of the underlying mechanisms is needed to improve rooting treatments. We first established a system to study rooting in Arabidopsis, the model organism in plant biology but only occasionally used to study adventitious rooting. Inhibition of polar auxin transport reduced AR formation. The role of auxin transporter proteins (several PIN-proteins) was found to be tissue-specific. Maturation (the transition from juvenile to adult) negatively influenced AR formation. Maturation was associated with increased DNA methylation and decreased miR156 level. 5-Azacytidine, a drug that reduces DNA methylation, increased rooting. We also examined the effect of two donor plant pre-treatments, etiolation and flooding, on rooting. Both increased AR formation.
AB - Adventitious root (AR) formation is indispensable in vegetative propagation and is widely used. A better understanding of the underlying mechanisms is needed to improve rooting treatments. We first established a system to study rooting in Arabidopsis, the model organism in plant biology but only occasionally used to study adventitious rooting. Inhibition of polar auxin transport reduced AR formation. The role of auxin transporter proteins (several PIN-proteins) was found to be tissue-specific. Maturation (the transition from juvenile to adult) negatively influenced AR formation. Maturation was associated with increased DNA methylation and decreased miR156 level. 5-Azacytidine, a drug that reduces DNA methylation, increased rooting. We also examined the effect of two donor plant pre-treatments, etiolation and flooding, on rooting. Both increased AR formation.
KW - arabidopsis thaliana
KW - adventitious roots
KW - formation
KW - plant development
KW - quantitative traits
KW - etiolation
KW - auxins
KW - explants
KW - molecular biology
KW - gene expression
KW - dna methylation
KW - rooting
KW - ontogeny
KW - plant breeding
KW - arabidopsis thaliana
KW - adventiefwortels
KW - formatie
KW - plantenontwikkeling
KW - kwantitatieve kenmerken
KW - etiolering
KW - auxinen
KW - explantaten
KW - moleculaire biologie
KW - genexpressie
KW - dna-methylering
KW - beworteling
KW - ontogenie
KW - plantenveredeling
U2 - 10.18174/385310
DO - 10.18174/385310
M3 - internal PhD, WU
PB - Wageningen University
CY - Wageningen
ER -