Advances in testing seed health

Peter Bonants*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

Abstract

Many different plant pathogens can be seed transmitted. In a world of global trade seeds infected with different plant pests are a potential risk. Seed health testing is important to prevent the spread of diseases. For the detection of these seed transmitted pests many methods are available e.g. ELISA, IF, dilution plating, PCR and real-time PCR. This chapter discusses several methods for seed health testing. It also evaluates the use of NGS sequencing for the detection of the most important seed transmitted pathogens in tomato: Clavibacter michiganensis subsp. michiganensis, Xanthomonas euvesicatoria, X. perforans, X. vesicatoria and X. gardneri, Pseudomonas syringae pv. tomato, Pepino Mosaic Virus and Potato spindle tuber viroid. These methods are compared with TaqMan PCR methods.
Original languageEnglish
Title of host publicationAdvances in seed science and technology for more sustainable crop production
EditorsJ. Buitink, O. Prince
Place of PublicationCambridge
PublisherBurleigh Dodds Science Publishing
Pages233-264
ISBN (Print)9781801465465
DOIs
Publication statusPublished - 26 Jul 2022

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