Adulteration of cow's milk with buffalo's milk detected by an on-site carbon nanoparticles-based lateral flow immunoassay

Rajan Sharma*, Archana Verma, Nitin Shinde, Bimlesh Mann, Kamal Gandhi, Jan H. Wichers, Aart van Amerongen

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

A competitive lateral flow immunoassay using amorphous carbon nanoparticles (CNPs) and non-immunoglobulin antigen has been developed for the rapid detection of adulteration of cow's milk with buffalo's milk. Purified polyclonal antibodies against a specific buffalo's milk protein fraction were conjugated to CNPs and sprayed on a conjugate pad. The test line consisted of buffalo's skimmed milk proteins (1.6 μg/cm), while the control line contained anti-rabbit antibodies raised in goat (0.5 μg/cm). In the test procedure milk sample is mixed with 100 mM borate buffer (pH 8.8 containing 1% BSA and 0.05% Tween 20) and pipetted onto the sample-cum-conjugate pad. A black/grey test line can be observed if the sample is free from buffalo's milk. The sensitivity of the test i.e. no visible test line is 5% adulteration of cow's milk with buffalo's milk. The test has applicability at the milk receiving stations and can be applied to heated milk samples.

Original languageEnglish
Article number129311
JournalFood Chemistry
Volume351
DOIs
Publication statusPublished - 30 Jul 2021

Keywords

  • Adulteration
  • Buffalo's milk
  • Carbon nanoparticles
  • Cow's milk
  • Lateral flow immunoassay

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