Adrenergic regulation of the innate immune response in common carp (Cyprinus carpio L.)

M.K. Chadzinska, E. Tertil, M. Kepka, G.J. Hermsen, M.H. Scheer, B.M.L. Verburg-van Kemenade

Research output: Contribution to journalArticleAcademicpeer-review

28 Citations (Scopus)

Abstract

Catecholamines exert their physiological actions through a and ß adrenergic receptors (ARs). As ARs are not exclusively expressed on neuroendocrine cells, but also on leukocytes, they may facilitate neuroendocrine modulation of immune responses. We sequenced the ß2a-AR in common carp, and studied its expression profile and involvement in the regulation of teleost innate immune responses. ß2a-AR messenger RNA was found to be constitutively expressed in brain areas, especially in the preoptic nucleus (NPO, homologous to the mammalian hypothalamus), and in immune organs. During the active phase of an in vivo inflammatory response, induced by i.p. zymosan treatment, ß2a-AR gene expression was up-regulated in the peritoneal leukocytes. Additionally, adrenaline in vitro reduced the synthesis of oxygen radical species and nitric oxide, while it enhanced arginase activity in fish phagocytes. Furthermore, in vitro adrenaline administration inhibited expression of pro-inflammatory cytokines, chemokines and their receptors. It is therefore hypothesized that adrenaline will down-regulate phagocyte skewing toward classical/innate polarization.
Original languageEnglish
Pages (from-to)306-316
JournalDevelopmental and Comparative Immunology
Volume36
Issue number2
DOIs
Publication statusPublished - 2012

Fingerprint

Carps
Innate Immunity
Adrenergic Agents
Adrenergic Receptors
Epinephrine
Phagocytes
Leukocytes
Arginase
Neuroendocrine Cells
Preoptic Area
Zymosan
Chemokine Receptors
Hypothalamus
Catecholamines
Reactive Oxygen Species
Nitric Oxide
Fishes
Down-Regulation
Cytokines
Gene Expression

Keywords

  • trout oncorhynchus-mykiss
  • necrosis-factor-alpha
  • rainbow-trout
  • beta-adrenoceptors
  • in-vitro
  • beta(2)-adrenergic receptor
  • neutrophilic granulocytes
  • interleukin-10 production
  • corticosteroid receptors
  • catecholamine secretion

Cite this

Chadzinska, M.K. ; Tertil, E. ; Kepka, M. ; Hermsen, G.J. ; Scheer, M.H. ; Verburg-van Kemenade, B.M.L. / Adrenergic regulation of the innate immune response in common carp (Cyprinus carpio L.). In: Developmental and Comparative Immunology. 2012 ; Vol. 36, No. 2. pp. 306-316.
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abstract = "Catecholamines exert their physiological actions through a and {\ss} adrenergic receptors (ARs). As ARs are not exclusively expressed on neuroendocrine cells, but also on leukocytes, they may facilitate neuroendocrine modulation of immune responses. We sequenced the {\ss}2a-AR in common carp, and studied its expression profile and involvement in the regulation of teleost innate immune responses. {\ss}2a-AR messenger RNA was found to be constitutively expressed in brain areas, especially in the preoptic nucleus (NPO, homologous to the mammalian hypothalamus), and in immune organs. During the active phase of an in vivo inflammatory response, induced by i.p. zymosan treatment, {\ss}2a-AR gene expression was up-regulated in the peritoneal leukocytes. Additionally, adrenaline in vitro reduced the synthesis of oxygen radical species and nitric oxide, while it enhanced arginase activity in fish phagocytes. Furthermore, in vitro adrenaline administration inhibited expression of pro-inflammatory cytokines, chemokines and their receptors. It is therefore hypothesized that adrenaline will down-regulate phagocyte skewing toward classical/innate polarization.",
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Adrenergic regulation of the innate immune response in common carp (Cyprinus carpio L.). / Chadzinska, M.K.; Tertil, E.; Kepka, M.; Hermsen, G.J.; Scheer, M.H.; Verburg-van Kemenade, B.M.L.

In: Developmental and Comparative Immunology, Vol. 36, No. 2, 2012, p. 306-316.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Scheer, M.H.

AU - Verburg-van Kemenade, B.M.L.

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N2 - Catecholamines exert their physiological actions through a and ß adrenergic receptors (ARs). As ARs are not exclusively expressed on neuroendocrine cells, but also on leukocytes, they may facilitate neuroendocrine modulation of immune responses. We sequenced the ß2a-AR in common carp, and studied its expression profile and involvement in the regulation of teleost innate immune responses. ß2a-AR messenger RNA was found to be constitutively expressed in brain areas, especially in the preoptic nucleus (NPO, homologous to the mammalian hypothalamus), and in immune organs. During the active phase of an in vivo inflammatory response, induced by i.p. zymosan treatment, ß2a-AR gene expression was up-regulated in the peritoneal leukocytes. Additionally, adrenaline in vitro reduced the synthesis of oxygen radical species and nitric oxide, while it enhanced arginase activity in fish phagocytes. Furthermore, in vitro adrenaline administration inhibited expression of pro-inflammatory cytokines, chemokines and their receptors. It is therefore hypothesized that adrenaline will down-regulate phagocyte skewing toward classical/innate polarization.

AB - Catecholamines exert their physiological actions through a and ß adrenergic receptors (ARs). As ARs are not exclusively expressed on neuroendocrine cells, but also on leukocytes, they may facilitate neuroendocrine modulation of immune responses. We sequenced the ß2a-AR in common carp, and studied its expression profile and involvement in the regulation of teleost innate immune responses. ß2a-AR messenger RNA was found to be constitutively expressed in brain areas, especially in the preoptic nucleus (NPO, homologous to the mammalian hypothalamus), and in immune organs. During the active phase of an in vivo inflammatory response, induced by i.p. zymosan treatment, ß2a-AR gene expression was up-regulated in the peritoneal leukocytes. Additionally, adrenaline in vitro reduced the synthesis of oxygen radical species and nitric oxide, while it enhanced arginase activity in fish phagocytes. Furthermore, in vitro adrenaline administration inhibited expression of pro-inflammatory cytokines, chemokines and their receptors. It is therefore hypothesized that adrenaline will down-regulate phagocyte skewing toward classical/innate polarization.

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