Adipose triglyceride lipase (ATGL) expression in human skeletal muscle is type I (oxidative) fiber specific

Johan W.E. Jocken, Egbert Smit, Gijs H. Goossens, Yvonne P.G. Essers, Marleen A. Van Baak, Marco Mensink, Wim H.M. Saris, Ellen E. Blaak

Research output: Contribution to journalArticleAcademicpeer-review

54 Citations (Scopus)

Abstract

Accumulation of triacylglycerol (TAG) and lipid intermediates in skeletal muscle plays an important role in the etiology of insulin resistance and type 2 diabetes mellitus. Disturbances in skeletal muscle lipid turnover and lipolysis may contribute significantly to this. So far, knowledge on the regulation of muscle lipolysis is limited. Recently the identification of a new lipase was reported: adipose triglyceride lipase (ATGL). ATGL deficient animals show significant lipid accumulation in skeletal muscle, which may indicate that ATGL plays a pivotal role in skeletal muscle lipolysis. However, until now, it is still unknown whether ATGL protein is expressed in human skeletal muscle. Therefore, the aim of the present study was to investigate whether ATGL is expressed at the protein level in human skeletal muscle, and to examine whether its expression is fiber-type specific. To accomplish this, we established an imunohistochemical and immunofluorescent staining procedure to study ATGL protein expression in relation to fiber type in human vastus lateralis muscle of eight male subjects (BMI range: 21.0-34.5 kg/m2 and age: 38-59 years). In the present paper we report for the first time that ATGL protein is indeed expressed in human skeletal muscle. Moreover, ATGL is exclusively expressed in type I (oxidative) muscle fibers, suggesting a pivotal role for ATGL in intramuscular fatty acid handling, lipid storage and breakdown.

Original languageEnglish
Pages (from-to)535-538
Number of pages4
JournalHistochemistry and Cell Biology
Volume129
Issue number4
DOIs
Publication statusPublished - 1 Apr 2008
Externally publishedYes

Keywords

  • ATGL
  • Fiber type
  • Obesity
  • Protein
  • Skeletal muscle

Fingerprint

Dive into the research topics of 'Adipose triglyceride lipase (ATGL) expression in human skeletal muscle is type I (oxidative) fiber specific'. Together they form a unique fingerprint.

Cite this