Adipose gene expression response of lean and obese mice to short-term dietary restriction

E.M. van Schothorst, J. Keijer, J.L.A. Pennings, A. Opperhuizen, C.E. van den Brom, T. Kohl, N.L.W. van Franssen-Hal, B. Hoebee

    Research output: Contribution to journalArticleAcademicpeer-review

    12 Citations (Scopus)

    Abstract

    Overweight and obesity lead to higher morbidity risks, which are alleviated even by mild weight loss. To gain insight in the molecular effects of weight loss in adipose tissue, we analyzed the effects of short-term dietary restriction (DR) on mice fed a low-fat diet (lean mice) or a high-fat diet (obese mice). Female C57Bl6/J mice on both diets were on DR until an average body weight loss of 20%, which was achieved in 8 to 12 days depending on body weight at the start of DR. Plasma free fatty acids and blood glucose levels decreased significantly on DR. In the (restricted) low-fat diet groups, gene expression analysis using adipose-enriched cDNA microarrays revealed only two transcripts to be significant differentially expressed by DR: up-regulation of malic enzyme (Mod1) and down-regulation of major urinary protein 1 (Mup1). Real-time polymerase chain reaction analysis confirmed these findings and showed, for the high-fat diet groups, an identical expression pattern for Mup1, whereas Mod1 showed an opposed gene expression pattern for the high-fat diet groups. In conclusion, initial weight loss induces transcriptional changes only in a very small number of adipose genes, which also depends on the (restricted) diet used
    Original languageEnglish
    Pages (from-to)974-979
    JournalObesity
    Volume14
    Issue number6
    DOIs
    Publication statusPublished - 2006

    Fingerprint

    Obese Mice
    Weight Loss
    High Fat Diet
    Gene Expression
    Fat-Restricted Diet
    Body Weight
    Diet
    Oligonucleotide Array Sequence Analysis
    Nonesterified Fatty Acids
    Blood Glucose
    Adipose Tissue
    Real-Time Polymerase Chain Reaction
    Up-Regulation
    Down-Regulation
    Obesity
    Molecular Weight
    Morbidity
    Enzymes
    Genes
    major urinary proteins

    Keywords

    • modest weight-loss
    • patterns
    • tissue
    • risk
    • rats

    Cite this

    van Schothorst, E. M., Keijer, J., Pennings, J. L. A., Opperhuizen, A., van den Brom, C. E., Kohl, T., ... Hoebee, B. (2006). Adipose gene expression response of lean and obese mice to short-term dietary restriction. Obesity, 14(6), 974-979. https://doi.org/10.1038/oby.2006.111
    van Schothorst, E.M. ; Keijer, J. ; Pennings, J.L.A. ; Opperhuizen, A. ; van den Brom, C.E. ; Kohl, T. ; van Franssen-Hal, N.L.W. ; Hoebee, B. / Adipose gene expression response of lean and obese mice to short-term dietary restriction. In: Obesity. 2006 ; Vol. 14, No. 6. pp. 974-979.
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    abstract = "Overweight and obesity lead to higher morbidity risks, which are alleviated even by mild weight loss. To gain insight in the molecular effects of weight loss in adipose tissue, we analyzed the effects of short-term dietary restriction (DR) on mice fed a low-fat diet (lean mice) or a high-fat diet (obese mice). Female C57Bl6/J mice on both diets were on DR until an average body weight loss of 20{\%}, which was achieved in 8 to 12 days depending on body weight at the start of DR. Plasma free fatty acids and blood glucose levels decreased significantly on DR. In the (restricted) low-fat diet groups, gene expression analysis using adipose-enriched cDNA microarrays revealed only two transcripts to be significant differentially expressed by DR: up-regulation of malic enzyme (Mod1) and down-regulation of major urinary protein 1 (Mup1). Real-time polymerase chain reaction analysis confirmed these findings and showed, for the high-fat diet groups, an identical expression pattern for Mup1, whereas Mod1 showed an opposed gene expression pattern for the high-fat diet groups. In conclusion, initial weight loss induces transcriptional changes only in a very small number of adipose genes, which also depends on the (restricted) diet used",
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    van Schothorst, EM, Keijer, J, Pennings, JLA, Opperhuizen, A, van den Brom, CE, Kohl, T, van Franssen-Hal, NLW & Hoebee, B 2006, 'Adipose gene expression response of lean and obese mice to short-term dietary restriction' Obesity, vol. 14, no. 6, pp. 974-979. https://doi.org/10.1038/oby.2006.111

    Adipose gene expression response of lean and obese mice to short-term dietary restriction. / van Schothorst, E.M.; Keijer, J.; Pennings, J.L.A.; Opperhuizen, A.; van den Brom, C.E.; Kohl, T.; van Franssen-Hal, N.L.W.; Hoebee, B.

    In: Obesity, Vol. 14, No. 6, 2006, p. 974-979.

    Research output: Contribution to journalArticleAcademicpeer-review

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    AU - Keijer, J.

    AU - Pennings, J.L.A.

    AU - Opperhuizen, A.

    AU - van den Brom, C.E.

    AU - Kohl, T.

    AU - van Franssen-Hal, N.L.W.

    AU - Hoebee, B.

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    AB - Overweight and obesity lead to higher morbidity risks, which are alleviated even by mild weight loss. To gain insight in the molecular effects of weight loss in adipose tissue, we analyzed the effects of short-term dietary restriction (DR) on mice fed a low-fat diet (lean mice) or a high-fat diet (obese mice). Female C57Bl6/J mice on both diets were on DR until an average body weight loss of 20%, which was achieved in 8 to 12 days depending on body weight at the start of DR. Plasma free fatty acids and blood glucose levels decreased significantly on DR. In the (restricted) low-fat diet groups, gene expression analysis using adipose-enriched cDNA microarrays revealed only two transcripts to be significant differentially expressed by DR: up-regulation of malic enzyme (Mod1) and down-regulation of major urinary protein 1 (Mup1). Real-time polymerase chain reaction analysis confirmed these findings and showed, for the high-fat diet groups, an identical expression pattern for Mup1, whereas Mod1 showed an opposed gene expression pattern for the high-fat diet groups. In conclusion, initial weight loss induces transcriptional changes only in a very small number of adipose genes, which also depends on the (restricted) diet used

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