Adhesion and formation of microbial biofilms in complex microfluidic devices

Aloke Kumar, David Karig, Suresh Neethirajan, Anil K. Suresh, Bernadeta R. Srijanto, Partha P. Mukherjee, Scott Retterer, Mitchel J. Doktycz

Research output: Chapter in Book/Report/Conference proceedingConference paperAcademicpeer-review

3 Citations (Scopus)

Abstract

Shewanella oneidensis is a metal reducing bacterium, which is of interest for bioremediation and clean energy applications. S. oneidensis biofilms play a critical role in several situations such as in microbial energy harvesting devices. Here, we use a microfluidic device to quantify the effects of hydrodynamics on the biofilm morphology of S. oneidensis. For different rates of fluid flow through a complex microfluidic device, we studied the spatiotemporal dynamics of biofilms, and we quantified several morphological features such as spatial distribution, cluster formation and surface coverage. We found that hydrodynamics resulted in significant differences in biofilm dynamics. The baffles in the device created regions of low and high flow in the same device. At higher flow rates, a nonuniform biofilm develops, due to unequal advection in different regions of the microchannel. However, at lower flow rates, a more uniform biofilm evolved. This depicts competition between adhesion events, growth and fluid advection. Atomic force microscopy (AFM) revealed that higher production of extra-cellular polymeric substances (EPS) occurred at higher flow velocities.

Original languageEnglish
Title of host publicationASME 2012 3rd International Conference on Micro/Nanoscale Heat and Mass Transfer, MNHMT 2012
Pages79-84
Number of pages6
DOIs
Publication statusPublished - 18 Jul 2013
Externally publishedYes
EventASME 2012 3rd International Conference on Micro/Nanoscale Heat and Mass Transfer, MNHMT 2012 - Atlanta, GA, United States
Duration: 3 Mar 20126 Mar 2012

Publication series

NameASME 2012 3rd International Conference on Micro/Nanoscale Heat and Mass Transfer, MNHMT 2012

Conference

ConferenceASME 2012 3rd International Conference on Micro/Nanoscale Heat and Mass Transfer, MNHMT 2012
CountryUnited States
CityAtlanta, GA
Period3/03/126/03/12

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