Adaptive braking by Ase1 prevents overlapping microtubules from sliding completely apart.

M. Braun, Z. Lansky, G. Fink, F. Ruhnow, S. Diez, M.E. Janson

Research output: Contribution to journalArticleAcademicpeer-review

56 Citations (Scopus)

Abstract

Short regions of overlap between ends of antiparallel microtubules are central elements within bipolar microtubule arrays. Although their formation requires motors1, recent in vitro studies demonstrated that stable overlaps cannot be generated by molecular motors alone. Motors either slide microtubules along each other until complete separation2, 3, 4 or, in the presence of opposing motors, generate oscillatory movements5, 6, 7. Here, we show that Ase1, a member of the conserved MAP65/PRC1 family of microtubule-bundling proteins, enables the formation of stable antiparallel overlaps through adaptive braking of Kinesin-14-driven microtubule–microtubule sliding. As overlapping microtubules start to slide apart, Ase1 molecules become compacted in the shrinking overlap and the sliding velocity gradually decreases in a dose-dependent manner. Compaction is driven by moving microtubule ends that act as barriers to Ase1 diffusion. Quantitative modelling showed that the molecular off-rate of Ase1 is sufficiently low to enable persistent overlap stabilization over tens of minutes. The finding of adaptive braking demonstrates that sliding can be slowed down locally to stabilize overlaps at the centre of bipolar arrays, whereas sliding proceeds elsewhere to enable network self-organization.
Original languageEnglish
Pages (from-to)1259-1264
Number of pages6
JournalNature Cell Biology
Volume13
DOIs
Publication statusPublished - 2011

Fingerprint

Microtubules
Microtubule Proteins
Kinesin

Keywords

  • fission yeast
  • spindle midzone
  • cross-linkers
  • protein
  • kinesin-14
  • binding
  • motors
  • prc1
  • organization
  • cytokinesis

Cite this

Braun, M. ; Lansky, Z. ; Fink, G. ; Ruhnow, F. ; Diez, S. ; Janson, M.E. / Adaptive braking by Ase1 prevents overlapping microtubules from sliding completely apart. In: Nature Cell Biology. 2011 ; Vol. 13. pp. 1259-1264.
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abstract = "Short regions of overlap between ends of antiparallel microtubules are central elements within bipolar microtubule arrays. Although their formation requires motors1, recent in vitro studies demonstrated that stable overlaps cannot be generated by molecular motors alone. Motors either slide microtubules along each other until complete separation2, 3, 4 or, in the presence of opposing motors, generate oscillatory movements5, 6, 7. Here, we show that Ase1, a member of the conserved MAP65/PRC1 family of microtubule-bundling proteins, enables the formation of stable antiparallel overlaps through adaptive braking of Kinesin-14-driven microtubule–microtubule sliding. As overlapping microtubules start to slide apart, Ase1 molecules become compacted in the shrinking overlap and the sliding velocity gradually decreases in a dose-dependent manner. Compaction is driven by moving microtubule ends that act as barriers to Ase1 diffusion. Quantitative modelling showed that the molecular off-rate of Ase1 is sufficiently low to enable persistent overlap stabilization over tens of minutes. The finding of adaptive braking demonstrates that sliding can be slowed down locally to stabilize overlaps at the centre of bipolar arrays, whereas sliding proceeds elsewhere to enable network self-organization.",
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Adaptive braking by Ase1 prevents overlapping microtubules from sliding completely apart. / Braun, M.; Lansky, Z.; Fink, G.; Ruhnow, F.; Diez, S.; Janson, M.E.

In: Nature Cell Biology, Vol. 13, 2011, p. 1259-1264.

Research output: Contribution to journalArticleAcademicpeer-review

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