Activity of Bacillis thuringiensis toxins against cocoa pod borer larvae

D. Santoso, T. Chaidamsari, S. Wiryadiputra, R.A. de Maagd

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

Twelve Cry proteins from Bacillus thuringiensis Berliner were tested in bioassays on cacao plantations in Indonesia for activity against the larvae of cocoa pod borer (Conopomorpha cramerella (Snellen)), an insect pest of the cacao tree. Through the damage caused by their feeding, the larvae of cocoa pod borer cause the pods of the cocoa tree to ripen prematurely. They are difficult to control with conventional measures. Preliminary assays identified five toxins that were more active than others. In two subsequent bioassays the activity of selected toxins was determined more accurately. Three Cry1 proteins with relatively little homology were all found to be toxic, opening perspectives for controlling cocoa pod borer by expression of Cry proteins in transgenic plants
Original languageEnglish
Pages (from-to)735-738
JournalPest Management Science
Volume60
Issue number8
DOIs
Publication statusPublished - 2004

Fingerprint

Conopomorpha cramerella
toxins
larvae
bioassays
cocoa pods
Bacillus thuringiensis
insect pests
Indonesia
transgenic plants
proteins
protein synthesis
plantations
assays

Keywords

  • brush-border membrane
  • crystal proteins
  • domain iii
  • spodoptera-exigua
  • lepidoptera
  • resistance
  • management
  • binding

Cite this

Santoso, D. ; Chaidamsari, T. ; Wiryadiputra, S. ; de Maagd, R.A. / Activity of Bacillis thuringiensis toxins against cocoa pod borer larvae. In: Pest Management Science. 2004 ; Vol. 60, No. 8. pp. 735-738.
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abstract = "Twelve Cry proteins from Bacillus thuringiensis Berliner were tested in bioassays on cacao plantations in Indonesia for activity against the larvae of cocoa pod borer (Conopomorpha cramerella (Snellen)), an insect pest of the cacao tree. Through the damage caused by their feeding, the larvae of cocoa pod borer cause the pods of the cocoa tree to ripen prematurely. They are difficult to control with conventional measures. Preliminary assays identified five toxins that were more active than others. In two subsequent bioassays the activity of selected toxins was determined more accurately. Three Cry1 proteins with relatively little homology were all found to be toxic, opening perspectives for controlling cocoa pod borer by expression of Cry proteins in transgenic plants",
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Activity of Bacillis thuringiensis toxins against cocoa pod borer larvae. / Santoso, D.; Chaidamsari, T.; Wiryadiputra, S.; de Maagd, R.A.

In: Pest Management Science, Vol. 60, No. 8, 2004, p. 735-738.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - de Maagd, R.A.

PY - 2004

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