Active defence responses associated with non-host resistance of Arabidopsis thaliana to the oomycete pathogen Phytophthora infestans

E. Huitema, V.G.A.A. Vleeshouwers, D.M. Francis, S. Kamoun

Research output: Contribution to journalArticleAcademicpeer-review

82 Citations (Scopus)

Abstract

The molecular basis of non-host resistance, or species-specific resistance, remains one of the major unknowns in the study of plant¿microbe interactions. In this paper, we describe the characterization of a non-host pathosystem involving the model plant Arabidopsis thaliana and the economically important and destructive oomycete pathogen Phytophthora infestans. Cytological investigations into the early stages of this interaction revealed the germination of P. infestans cysts on Arabidopsis leaves, direct penetration of epidermal cells, formation of infection vesicles and occasionally secondary hyphae, followed by a typical hypersensitive response. P. infestans biomass dynamics during infection of Arabidopsis was monitored using kinetic PCR, revealing an increase in biomass during the first 24 h after inoculation, followed by a decrease in the later stages. Transgenic reporter lines and RNA blot analyses were used to characterize the defence responses induced following P. infestans infection. Significant induction of PDF1.2 was observed at 48 h after inoculation, whereas elevated levels of PR gene expression were detected three days after inoculation. To further characterize this defence response, DNA microarray analyses were carried out to determine the expression profiles for c. 11 000 Arabidopsis cDNAs 16 h after infection. These analyses revealed a significant overlap between Arabidopsis non-host response and other defence-related treatments described in the literature. In particular, non-host response to P. infestans was clearly associated with activation of the jasmonate pathway. The described Arabidopsis¿P. infestans pathosystem offers excellent prospects for improving our understanding of non-host resistance.
Original languageEnglish
Pages (from-to)487-500
JournalMolecular Plant Pathology
Volume4
Issue number6
DOIs
Publication statusPublished - 2003

Fingerprint

Phytophthora infestans
Oomycetes
Arabidopsis
Arabidopsis thaliana
pathogen
inoculation
pathogens
Infection
infection
Biomass
induced response
biomass
cyst
vesicle
Hyphae
gene expression
RNA
hypersensitive response
Microarray Analysis
germination

Keywords

  • systemic acquired-resistance
  • cultured parsley cells
  • disease resistance
  • salicylic-acid
  • peronospora-parasitica
  • hypersensitive response
  • innate immunity
  • molecular-genetics
  • oxidative burst
  • plant defense

Cite this

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title = "Active defence responses associated with non-host resistance of Arabidopsis thaliana to the oomycete pathogen Phytophthora infestans",
abstract = "The molecular basis of non-host resistance, or species-specific resistance, remains one of the major unknowns in the study of plant¿microbe interactions. In this paper, we describe the characterization of a non-host pathosystem involving the model plant Arabidopsis thaliana and the economically important and destructive oomycete pathogen Phytophthora infestans. Cytological investigations into the early stages of this interaction revealed the germination of P. infestans cysts on Arabidopsis leaves, direct penetration of epidermal cells, formation of infection vesicles and occasionally secondary hyphae, followed by a typical hypersensitive response. P. infestans biomass dynamics during infection of Arabidopsis was monitored using kinetic PCR, revealing an increase in biomass during the first 24 h after inoculation, followed by a decrease in the later stages. Transgenic reporter lines and RNA blot analyses were used to characterize the defence responses induced following P. infestans infection. Significant induction of PDF1.2 was observed at 48 h after inoculation, whereas elevated levels of PR gene expression were detected three days after inoculation. To further characterize this defence response, DNA microarray analyses were carried out to determine the expression profiles for c. 11 000 Arabidopsis cDNAs 16 h after infection. These analyses revealed a significant overlap between Arabidopsis non-host response and other defence-related treatments described in the literature. In particular, non-host response to P. infestans was clearly associated with activation of the jasmonate pathway. The described Arabidopsis¿P. infestans pathosystem offers excellent prospects for improving our understanding of non-host resistance.",
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Active defence responses associated with non-host resistance of Arabidopsis thaliana to the oomycete pathogen Phytophthora infestans. / Huitema, E.; Vleeshouwers, V.G.A.A.; Francis, D.M.; Kamoun, S.

In: Molecular Plant Pathology, Vol. 4, No. 6, 2003, p. 487-500.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Active defence responses associated with non-host resistance of Arabidopsis thaliana to the oomycete pathogen Phytophthora infestans

AU - Huitema, E.

AU - Vleeshouwers, V.G.A.A.

AU - Francis, D.M.

AU - Kamoun, S.

N1 - 000220169600007

PY - 2003

Y1 - 2003

N2 - The molecular basis of non-host resistance, or species-specific resistance, remains one of the major unknowns in the study of plant¿microbe interactions. In this paper, we describe the characterization of a non-host pathosystem involving the model plant Arabidopsis thaliana and the economically important and destructive oomycete pathogen Phytophthora infestans. Cytological investigations into the early stages of this interaction revealed the germination of P. infestans cysts on Arabidopsis leaves, direct penetration of epidermal cells, formation of infection vesicles and occasionally secondary hyphae, followed by a typical hypersensitive response. P. infestans biomass dynamics during infection of Arabidopsis was monitored using kinetic PCR, revealing an increase in biomass during the first 24 h after inoculation, followed by a decrease in the later stages. Transgenic reporter lines and RNA blot analyses were used to characterize the defence responses induced following P. infestans infection. Significant induction of PDF1.2 was observed at 48 h after inoculation, whereas elevated levels of PR gene expression were detected three days after inoculation. To further characterize this defence response, DNA microarray analyses were carried out to determine the expression profiles for c. 11 000 Arabidopsis cDNAs 16 h after infection. These analyses revealed a significant overlap between Arabidopsis non-host response and other defence-related treatments described in the literature. In particular, non-host response to P. infestans was clearly associated with activation of the jasmonate pathway. The described Arabidopsis¿P. infestans pathosystem offers excellent prospects for improving our understanding of non-host resistance.

AB - The molecular basis of non-host resistance, or species-specific resistance, remains one of the major unknowns in the study of plant¿microbe interactions. In this paper, we describe the characterization of a non-host pathosystem involving the model plant Arabidopsis thaliana and the economically important and destructive oomycete pathogen Phytophthora infestans. Cytological investigations into the early stages of this interaction revealed the germination of P. infestans cysts on Arabidopsis leaves, direct penetration of epidermal cells, formation of infection vesicles and occasionally secondary hyphae, followed by a typical hypersensitive response. P. infestans biomass dynamics during infection of Arabidopsis was monitored using kinetic PCR, revealing an increase in biomass during the first 24 h after inoculation, followed by a decrease in the later stages. Transgenic reporter lines and RNA blot analyses were used to characterize the defence responses induced following P. infestans infection. Significant induction of PDF1.2 was observed at 48 h after inoculation, whereas elevated levels of PR gene expression were detected three days after inoculation. To further characterize this defence response, DNA microarray analyses were carried out to determine the expression profiles for c. 11 000 Arabidopsis cDNAs 16 h after infection. These analyses revealed a significant overlap between Arabidopsis non-host response and other defence-related treatments described in the literature. In particular, non-host response to P. infestans was clearly associated with activation of the jasmonate pathway. The described Arabidopsis¿P. infestans pathosystem offers excellent prospects for improving our understanding of non-host resistance.

KW - systemic acquired-resistance

KW - cultured parsley cells

KW - disease resistance

KW - salicylic-acid

KW - peronospora-parasitica

KW - hypersensitive response

KW - innate immunity

KW - molecular-genetics

KW - oxidative burst

KW - plant defense

U2 - 10.1046/j.1364-3703.2003.00195.x

DO - 10.1046/j.1364-3703.2003.00195.x

M3 - Article

VL - 4

SP - 487

EP - 500

JO - Molecular Plant Pathology

JF - Molecular Plant Pathology

SN - 1464-6722

IS - 6

ER -