Activation tagging of ATHB13 in Arabidopsis thaliana confers broad-spectrum disease resistance

D. Gao, R.P. Huibers, X. Chen, A.E.H.M. Loonen, R.G.F. Visser, A.M.A. Wolters, Y. Bai

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17 Citations (Scopus)

Abstract

Powdery mildew species Oidium neolycopersici (On) can cause serious yield losses in tomato production worldwide. Besides on tomato, On is able to grow and reproduce on Arabidopsis. In this study we screened a collection of activation-tagged Arabidopsis mutants and identified one mutant, 3221, which displayed resistance to On, and in addition showed a reduced stature and serrated leaves. Additional disease tests demonstrated that the 3221 mutant exhibited resistance to downy mildew (Hyaloperonospora arabidopsidis) and green peach aphid (Myzus persicae), but retained susceptibility to bacterial pathogen Pseudomonas syringae pv tomato DC3000. The resistance trait and morphological alteration were mutually linked in 3221. Identification of the activation tag insertion site and microarray analysis revealed that ATHB13, a homeodomain-leucine zipper (HD-Zip) transcription factor, was constitutively overexpressed in 3221. Silencing of ATHB13 in 3221 resulted in the loss of both the morphological alteration and resistance, whereas overexpression of the cloned ATHB13 in Col-0 and Col-eds1-2 backgrounds resulted in morphological alteration and resistance. Microarray analysis further revealed that overexpression of ATHB13 influenced the expression of a large number of genes. Previously, it was reported that ATHB13-overexpressing lines conferred tolerance to abiotic stress. Together with our results, it appears that ATHB13 is involved in the crosstalk between abiotic and biotic stress resistance pathways.
Original languageEnglish
Pages (from-to)641-653
JournalPlant Molecular Biology
Volume86
Issue number6
DOIs
Publication statusPublished - 2014

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Oidium neolycopersici
Disease Resistance
Lycopersicon esculentum
Arabidopsis
disease resistance
Aphids
Arabidopsis thaliana
Myzus persicae
tomatoes
Microarray Analysis
mutants
abiotic stress
Pseudomonas syringae
Leucine Zippers
leucine zipper
downy mildew
biotic stress
powdery mildew
stress tolerance
Transcription Factors

Keywords

  • vegetative storage protein
  • powdery mildew resistance
  • transcription factors hahb1
  • nudix hydrolase
  • salicylic-acid
  • plant defense
  • cell-death
  • hd-zip
  • oidium-neolycopersici
  • expression patterns

Cite this

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title = "Activation tagging of ATHB13 in Arabidopsis thaliana confers broad-spectrum disease resistance",
abstract = "Powdery mildew species Oidium neolycopersici (On) can cause serious yield losses in tomato production worldwide. Besides on tomato, On is able to grow and reproduce on Arabidopsis. In this study we screened a collection of activation-tagged Arabidopsis mutants and identified one mutant, 3221, which displayed resistance to On, and in addition showed a reduced stature and serrated leaves. Additional disease tests demonstrated that the 3221 mutant exhibited resistance to downy mildew (Hyaloperonospora arabidopsidis) and green peach aphid (Myzus persicae), but retained susceptibility to bacterial pathogen Pseudomonas syringae pv tomato DC3000. The resistance trait and morphological alteration were mutually linked in 3221. Identification of the activation tag insertion site and microarray analysis revealed that ATHB13, a homeodomain-leucine zipper (HD-Zip) transcription factor, was constitutively overexpressed in 3221. Silencing of ATHB13 in 3221 resulted in the loss of both the morphological alteration and resistance, whereas overexpression of the cloned ATHB13 in Col-0 and Col-eds1-2 backgrounds resulted in morphological alteration and resistance. Microarray analysis further revealed that overexpression of ATHB13 influenced the expression of a large number of genes. Previously, it was reported that ATHB13-overexpressing lines conferred tolerance to abiotic stress. Together with our results, it appears that ATHB13 is involved in the crosstalk between abiotic and biotic stress resistance pathways.",
keywords = "vegetative storage protein, powdery mildew resistance, transcription factors hahb1, nudix hydrolase, salicylic-acid, plant defense, cell-death, hd-zip, oidium-neolycopersici, expression patterns",
author = "D. Gao and R.P. Huibers and X. Chen and A.E.H.M. Loonen and R.G.F. Visser and A.M.A. Wolters and Y. Bai",
year = "2014",
doi = "10.1007/s11103-014-0253-2",
language = "English",
volume = "86",
pages = "641--653",
journal = "Plant Molecular Biology",
issn = "0167-4412",
publisher = "Springer Verlag",
number = "6",

}

Activation tagging of ATHB13 in Arabidopsis thaliana confers broad-spectrum disease resistance. / Gao, D.; Huibers, R.P.; Chen, X.; Loonen, A.E.H.M.; Visser, R.G.F.; Wolters, A.M.A.; Bai, Y.

In: Plant Molecular Biology, Vol. 86, No. 6, 2014, p. 641-653.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Activation tagging of ATHB13 in Arabidopsis thaliana confers broad-spectrum disease resistance

AU - Gao, D.

AU - Huibers, R.P.

AU - Chen, X.

AU - Loonen, A.E.H.M.

AU - Visser, R.G.F.

AU - Wolters, A.M.A.

AU - Bai, Y.

PY - 2014

Y1 - 2014

N2 - Powdery mildew species Oidium neolycopersici (On) can cause serious yield losses in tomato production worldwide. Besides on tomato, On is able to grow and reproduce on Arabidopsis. In this study we screened a collection of activation-tagged Arabidopsis mutants and identified one mutant, 3221, which displayed resistance to On, and in addition showed a reduced stature and serrated leaves. Additional disease tests demonstrated that the 3221 mutant exhibited resistance to downy mildew (Hyaloperonospora arabidopsidis) and green peach aphid (Myzus persicae), but retained susceptibility to bacterial pathogen Pseudomonas syringae pv tomato DC3000. The resistance trait and morphological alteration were mutually linked in 3221. Identification of the activation tag insertion site and microarray analysis revealed that ATHB13, a homeodomain-leucine zipper (HD-Zip) transcription factor, was constitutively overexpressed in 3221. Silencing of ATHB13 in 3221 resulted in the loss of both the morphological alteration and resistance, whereas overexpression of the cloned ATHB13 in Col-0 and Col-eds1-2 backgrounds resulted in morphological alteration and resistance. Microarray analysis further revealed that overexpression of ATHB13 influenced the expression of a large number of genes. Previously, it was reported that ATHB13-overexpressing lines conferred tolerance to abiotic stress. Together with our results, it appears that ATHB13 is involved in the crosstalk between abiotic and biotic stress resistance pathways.

AB - Powdery mildew species Oidium neolycopersici (On) can cause serious yield losses in tomato production worldwide. Besides on tomato, On is able to grow and reproduce on Arabidopsis. In this study we screened a collection of activation-tagged Arabidopsis mutants and identified one mutant, 3221, which displayed resistance to On, and in addition showed a reduced stature and serrated leaves. Additional disease tests demonstrated that the 3221 mutant exhibited resistance to downy mildew (Hyaloperonospora arabidopsidis) and green peach aphid (Myzus persicae), but retained susceptibility to bacterial pathogen Pseudomonas syringae pv tomato DC3000. The resistance trait and morphological alteration were mutually linked in 3221. Identification of the activation tag insertion site and microarray analysis revealed that ATHB13, a homeodomain-leucine zipper (HD-Zip) transcription factor, was constitutively overexpressed in 3221. Silencing of ATHB13 in 3221 resulted in the loss of both the morphological alteration and resistance, whereas overexpression of the cloned ATHB13 in Col-0 and Col-eds1-2 backgrounds resulted in morphological alteration and resistance. Microarray analysis further revealed that overexpression of ATHB13 influenced the expression of a large number of genes. Previously, it was reported that ATHB13-overexpressing lines conferred tolerance to abiotic stress. Together with our results, it appears that ATHB13 is involved in the crosstalk between abiotic and biotic stress resistance pathways.

KW - vegetative storage protein

KW - powdery mildew resistance

KW - transcription factors hahb1

KW - nudix hydrolase

KW - salicylic-acid

KW - plant defense

KW - cell-death

KW - hd-zip

KW - oidium-neolycopersici

KW - expression patterns

U2 - 10.1007/s11103-014-0253-2

DO - 10.1007/s11103-014-0253-2

M3 - Article

VL - 86

SP - 641

EP - 653

JO - Plant Molecular Biology

JF - Plant Molecular Biology

SN - 0167-4412

IS - 6

ER -