Abstract
Soil-borne vascular wilt diseases caused by Verticillium spp. are among the most notorious diseases worldwide, causing severe yield and quality losses in food, feed and industrial crops. Regardless of the economical importance of Verticillium wilt disease, little is known about the molecular basis of Verticillium-host interactions and genes playing key role in these interactions. We have undertaken a forward genetics approach to identify host genes that play an important role in the interaction of Arabidopsis with Verticillium. To this end, an
activation tagged Arabidopsis mutant library was screened with V. dahliae. From this screening, four mutants with enhanced resistance were identified that also appeared to display enhanced resistance towards V. albo-atrum. The
enhanced resistance phenotypes have been confirmed by quantifying the fungal biomass in planta using real-time PCR. To further study whether the enhanced resistance of the mutants is specific to Verticillium spp. or also concerns other
pathogens, the mutants were challenged with the fungal pathogens Botrytis cinerea, Plectosphaerella cucumerina and Alternaria brassicicola, and the bacterial pathogen Pseudomonas syringae. Interestingly, the mutants displayed
differential responses towards these pathogens. Currently, analyses to identify the T-DNA insertion sites are ongoing. This research is supported by the Dutch Technology Foundation STW, applied science division of NWO and the
Technology Program of the Ministry of Economic Affairs.
activation tagged Arabidopsis mutant library was screened with V. dahliae. From this screening, four mutants with enhanced resistance were identified that also appeared to display enhanced resistance towards V. albo-atrum. The
enhanced resistance phenotypes have been confirmed by quantifying the fungal biomass in planta using real-time PCR. To further study whether the enhanced resistance of the mutants is specific to Verticillium spp. or also concerns other
pathogens, the mutants were challenged with the fungal pathogens Botrytis cinerea, Plectosphaerella cucumerina and Alternaria brassicicola, and the bacterial pathogen Pseudomonas syringae. Interestingly, the mutants displayed
differential responses towards these pathogens. Currently, analyses to identify the T-DNA insertion sites are ongoing. This research is supported by the Dutch Technology Foundation STW, applied science division of NWO and the
Technology Program of the Ministry of Economic Affairs.
| Original language | English |
|---|---|
| Title of host publication | ISMPMI International Congress abstracts, Quebec City, Canada, 19-23 July 2009 |
| Publisher | International Society for Molecular Plant-Microbe Interactions |
| Pages | 154 |
| Publication status | Published - 2009 |
| Event | XIV International Congress on Molecular Plant-Microbe Interactions, Quebec City, Canada - Duration: 19 Jul 2009 → 23 Jul 2009 |
Conference
| Conference | XIV International Congress on Molecular Plant-Microbe Interactions, Quebec City, Canada |
|---|---|
| Period | 19/07/09 → 23/07/09 |