Actin-Depolymerizing Factor2-Mediated Actin Dynamics Are Essential for Root-Knot Nematode Infection of Arabidopsis

M. Clement, T. Ketelaar, N. Rodiuc, M.Y. Banora, A. Smertenko, G. Engler, P. Abad, P.J. Hussey, J. De Almeida Engler

Research output: Contribution to journalArticleAcademicpeer-review

56 Citations (Scopus)

Abstract

Reorganization of the actin and microtubule networks is known to occur in targeted vascular parenchymal root cells upon infection with the nematode Meloidogyne incognita. Here, we show that actin-depolymerizing factor (ADF) is upregulated in the giant feeding cells of Arabidopsis thaliana that develop upon nematode infection and that knockdown of a specific ADF isotype inhibits nematode proliferation. Analysis of the levels of transcript and the localization of seven ADF genes shows that five are upregulated in galls that result from the infection and that ADF2 expression is particularly increased between 14 and 21 d after nematode inoculation. Further analysis of ADF2 function in inducible RNA interference lines designed to knock down ADF2 expression reveals that this protein is required for normal cell growth and plant development. The net effect of decreased levels of ADF2 is F-actin stabilization in cells, resulting from decreased F-actin turnover. In nematode-infected plants with reduced levels of ADF2, the galls containing the giant feeding cells and growing nematodes do not develop due to the arrest in growth of the giant multinucleate feeding cells, which in turn is due to an aberrant actin network
Original languageEnglish
Pages (from-to)2963-2979
Number of pages17
JournalThe Plant Cell
Volume21
Issue number9
DOIs
Publication statusPublished - 2009

    Fingerprint

Keywords

  • plant-parasitic nematodes
  • filament turnover
  • nonhost resistance
  • in-vivo
  • adf/cofilin family
  • gene-expression
  • cell periphery
  • maize actin
  • giant-cells
  • cytoskeleton

Cite this

Clement, M., Ketelaar, T., Rodiuc, N., Banora, M. Y., Smertenko, A., Engler, G., ... De Almeida Engler, J. (2009). Actin-Depolymerizing Factor2-Mediated Actin Dynamics Are Essential for Root-Knot Nematode Infection of Arabidopsis. The Plant Cell, 21(9), 2963-2979. https://doi.org/10.1105/tpc.109.069104