Across-line SNP association study of innate and adaptive immune response in laying hens

F. Biscarini, H. Bovenhuis, J.A.M. van Arendonk, H.K. Parmentier, B.J. Jungerius, J.J. van der Poel

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Abstract

The aim of the present study was to detect quantitative trait loci (QTL) for innate and adaptive immunity in laying hens. For this purpose, the associations between 1022 single nucleotide polymorphism (SNP) markers and immune traits were studied in 583 hens from nine different layer lines. Immune traits were natural antibodies for keyhole limpet haemocyanin (KLH) and lipopolysaccharide (LPS) at 20, 40 and 65 weeks, acquired antibodies to the vaccinal virus of Newcastle disease at 20 weeks, and complement activity measured on sheep and bovine red blood cells at 20, 40 and 65 weeks. We adopted a novel approach based on across-line analysis and testing of the SNP-by-line interaction. Among lines, linkage disequilibrium is conserved at shorter distances than in individual lines; therefore, SNPs significantly associated with immune traits across lines are expected to be near the functional mutations. In the analysis, the SNPs that had a significant across-line effect but did not show significant SNP-by-line interaction were identified to test whether the association was consistent in the individual lines. Ultimately, 59 significant associations between SNPs and immune traits were detected. Our results confirmed some previously identified QTL and identified new QTL potentially involved in the immune function. We found evidence for a role of IL17A (chromosome 3) in natural and acquired antibody titres and in the classical and alternative pathways of complement activation. The major histocompatibility genes on chromosome 16 showed significant association with natural and acquired antibody titres and classical complement activity. The IL12B gene on chromosome 13 was associated with natural antibody titres
Original languageEnglish
Pages (from-to)26-38
JournalAnimal Genetics
Volume41
Issue number1
DOIs
Publication statusPublished - 2010

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Adaptive Immunity
Innate Immunity
laying hens
single nucleotide polymorphism
Single Nucleotide Polymorphism
antibodies
Quantitative Trait Loci
quantitative trait loci
complement
Antibodies
chromosomes
Classical Complement Pathway
Alternative Complement Pathway
Newcastle disease virus
Chromosomes, Human, Pair 16
Chromosomes, Human, Pair 13
linkage disequilibrium
Histocompatibility
Chromosomes, Human, Pair 3
lipopolysaccharides

Keywords

  • quantitative trait loci
  • linkage disequilibrium
  • antibody-response
  • natural antibodies
  • chickens
  • populations
  • heritability
  • enteritidis
  • survival
  • disease

Cite this

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title = "Across-line SNP association study of innate and adaptive immune response in laying hens",
abstract = "The aim of the present study was to detect quantitative trait loci (QTL) for innate and adaptive immunity in laying hens. For this purpose, the associations between 1022 single nucleotide polymorphism (SNP) markers and immune traits were studied in 583 hens from nine different layer lines. Immune traits were natural antibodies for keyhole limpet haemocyanin (KLH) and lipopolysaccharide (LPS) at 20, 40 and 65 weeks, acquired antibodies to the vaccinal virus of Newcastle disease at 20 weeks, and complement activity measured on sheep and bovine red blood cells at 20, 40 and 65 weeks. We adopted a novel approach based on across-line analysis and testing of the SNP-by-line interaction. Among lines, linkage disequilibrium is conserved at shorter distances than in individual lines; therefore, SNPs significantly associated with immune traits across lines are expected to be near the functional mutations. In the analysis, the SNPs that had a significant across-line effect but did not show significant SNP-by-line interaction were identified to test whether the association was consistent in the individual lines. Ultimately, 59 significant associations between SNPs and immune traits were detected. Our results confirmed some previously identified QTL and identified new QTL potentially involved in the immune function. We found evidence for a role of IL17A (chromosome 3) in natural and acquired antibody titres and in the classical and alternative pathways of complement activation. The major histocompatibility genes on chromosome 16 showed significant association with natural and acquired antibody titres and classical complement activity. The IL12B gene on chromosome 13 was associated with natural antibody titres",
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author = "F. Biscarini and H. Bovenhuis and {van Arendonk}, J.A.M. and H.K. Parmentier and B.J. Jungerius and {van der Poel}, J.J.",
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Across-line SNP association study of innate and adaptive immune response in laying hens. / Biscarini, F.; Bovenhuis, H.; van Arendonk, J.A.M.; Parmentier, H.K.; Jungerius, B.J.; van der Poel, J.J.

In: Animal Genetics, Vol. 41, No. 1, 2010, p. 26-38.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Across-line SNP association study of innate and adaptive immune response in laying hens

AU - Biscarini, F.

AU - Bovenhuis, H.

AU - van Arendonk, J.A.M.

AU - Parmentier, H.K.

AU - Jungerius, B.J.

AU - van der Poel, J.J.

PY - 2010

Y1 - 2010

N2 - The aim of the present study was to detect quantitative trait loci (QTL) for innate and adaptive immunity in laying hens. For this purpose, the associations between 1022 single nucleotide polymorphism (SNP) markers and immune traits were studied in 583 hens from nine different layer lines. Immune traits were natural antibodies for keyhole limpet haemocyanin (KLH) and lipopolysaccharide (LPS) at 20, 40 and 65 weeks, acquired antibodies to the vaccinal virus of Newcastle disease at 20 weeks, and complement activity measured on sheep and bovine red blood cells at 20, 40 and 65 weeks. We adopted a novel approach based on across-line analysis and testing of the SNP-by-line interaction. Among lines, linkage disequilibrium is conserved at shorter distances than in individual lines; therefore, SNPs significantly associated with immune traits across lines are expected to be near the functional mutations. In the analysis, the SNPs that had a significant across-line effect but did not show significant SNP-by-line interaction were identified to test whether the association was consistent in the individual lines. Ultimately, 59 significant associations between SNPs and immune traits were detected. Our results confirmed some previously identified QTL and identified new QTL potentially involved in the immune function. We found evidence for a role of IL17A (chromosome 3) in natural and acquired antibody titres and in the classical and alternative pathways of complement activation. The major histocompatibility genes on chromosome 16 showed significant association with natural and acquired antibody titres and classical complement activity. The IL12B gene on chromosome 13 was associated with natural antibody titres

AB - The aim of the present study was to detect quantitative trait loci (QTL) for innate and adaptive immunity in laying hens. For this purpose, the associations between 1022 single nucleotide polymorphism (SNP) markers and immune traits were studied in 583 hens from nine different layer lines. Immune traits were natural antibodies for keyhole limpet haemocyanin (KLH) and lipopolysaccharide (LPS) at 20, 40 and 65 weeks, acquired antibodies to the vaccinal virus of Newcastle disease at 20 weeks, and complement activity measured on sheep and bovine red blood cells at 20, 40 and 65 weeks. We adopted a novel approach based on across-line analysis and testing of the SNP-by-line interaction. Among lines, linkage disequilibrium is conserved at shorter distances than in individual lines; therefore, SNPs significantly associated with immune traits across lines are expected to be near the functional mutations. In the analysis, the SNPs that had a significant across-line effect but did not show significant SNP-by-line interaction were identified to test whether the association was consistent in the individual lines. Ultimately, 59 significant associations between SNPs and immune traits were detected. Our results confirmed some previously identified QTL and identified new QTL potentially involved in the immune function. We found evidence for a role of IL17A (chromosome 3) in natural and acquired antibody titres and in the classical and alternative pathways of complement activation. The major histocompatibility genes on chromosome 16 showed significant association with natural and acquired antibody titres and classical complement activity. The IL12B gene on chromosome 13 was associated with natural antibody titres

KW - quantitative trait loci

KW - linkage disequilibrium

KW - antibody-response

KW - natural antibodies

KW - chickens

KW - populations

KW - heritability

KW - enteritidis

KW - survival

KW - disease

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DO - 10.1111/j.1365-2052.2009.01960.x

M3 - Article

VL - 41

SP - 26

EP - 38

JO - Animal Genetics

JF - Animal Genetics

SN - 0268-9146

IS - 1

ER -