Accumulation of human EGF in nectar of transformed plants of Nicotiana langsdorffii × N. sanderae and transfer to honey by bees

J.P.F.G. Helsper, C.P. Ruyter-Spira, P.H.S. Kwakman, W.K. Bleeker, L.C.P. Keizer, J.B. Bade, A.A. te Velde, S.A.J. Zaat, M. Verbeek, J. Creemers-Molenaar

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Honey has been used successfully in wound healing for thousands of years. The peptide hormone human epidermal growth factor (hEGF) is also known to have a beneficial effect in various wound healing processes via mechanisms that differ from those for honey. In this study, we show that hEGF can be incorporated into honey via nectar. Plants of Nicotiana langsdorffii × N. sanderae were transformed with the gene for hEGF, equipped with a nectary-targeted promoter and a signal sequence for secretion to nectar. These plants accumulated hEGF in the nectar. The maximum hEGF concentration recorded with ELISA in these plants is 2.5 ng·ml-1. There is a significant linear relationship (P <0.001) between hEGF concentration and induction of hEGF-receptor phosphorylation. Since the flower morphology of these plants did not allow production of honey from their nectar, we used feeding solutions, spiked with synthetic hEGF, to study transfer of this peptide into honey through bee activity. Transfer of hEGF from a feeding solution to honey by bees occurred with retention of the hEGF concentration and the capacity to induce hEGF-receptor phosphorylation. These observations indicate that plants can function as a production platform for honey containing biologically active peptides, which may enhance wound healing and other biological processes
Original languageEnglish
Pages (from-to)740-746
JournalPlant Biology
Volume13
Issue number5
DOIs
Publication statusPublished - 2011

Fingerprint

Nicotiana langsdorffii
nectar plants
honey
nectar
bee
epidermal growth factor
Apoidea
tissue repair
peptide
phosphorylation
peptides
nectary
peptide hormones
nectaries
plant morphology
signal peptide
biological processes
secretion

Keywords

  • epidermal-growth-factor
  • wound-healing process
  • floral sources
  • antioxidant capacity
  • apis-mellifera
  • in-vitro
  • cells
  • inhibition
  • expression
  • dressings

Cite this

Helsper, J.P.F.G. ; Ruyter-Spira, C.P. ; Kwakman, P.H.S. ; Bleeker, W.K. ; Keizer, L.C.P. ; Bade, J.B. ; te Velde, A.A. ; Zaat, S.A.J. ; Verbeek, M. ; Creemers-Molenaar, J. / Accumulation of human EGF in nectar of transformed plants of Nicotiana langsdorffii × N. sanderae and transfer to honey by bees. In: Plant Biology. 2011 ; Vol. 13, No. 5. pp. 740-746.
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abstract = "Honey has been used successfully in wound healing for thousands of years. The peptide hormone human epidermal growth factor (hEGF) is also known to have a beneficial effect in various wound healing processes via mechanisms that differ from those for honey. In this study, we show that hEGF can be incorporated into honey via nectar. Plants of Nicotiana langsdorffii × N. sanderae were transformed with the gene for hEGF, equipped with a nectary-targeted promoter and a signal sequence for secretion to nectar. These plants accumulated hEGF in the nectar. The maximum hEGF concentration recorded with ELISA in these plants is 2.5 ng·ml-1. There is a significant linear relationship (P <0.001) between hEGF concentration and induction of hEGF-receptor phosphorylation. Since the flower morphology of these plants did not allow production of honey from their nectar, we used feeding solutions, spiked with synthetic hEGF, to study transfer of this peptide into honey through bee activity. Transfer of hEGF from a feeding solution to honey by bees occurred with retention of the hEGF concentration and the capacity to induce hEGF-receptor phosphorylation. These observations indicate that plants can function as a production platform for honey containing biologically active peptides, which may enhance wound healing and other biological processes",
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author = "J.P.F.G. Helsper and C.P. Ruyter-Spira and P.H.S. Kwakman and W.K. Bleeker and L.C.P. Keizer and J.B. Bade and {te Velde}, A.A. and S.A.J. Zaat and M. Verbeek and J. Creemers-Molenaar",
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Accumulation of human EGF in nectar of transformed plants of Nicotiana langsdorffii × N. sanderae and transfer to honey by bees. / Helsper, J.P.F.G.; Ruyter-Spira, C.P.; Kwakman, P.H.S.; Bleeker, W.K.; Keizer, L.C.P.; Bade, J.B.; te Velde, A.A.; Zaat, S.A.J.; Verbeek, M.; Creemers-Molenaar, J.

In: Plant Biology, Vol. 13, No. 5, 2011, p. 740-746.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Accumulation of human EGF in nectar of transformed plants of Nicotiana langsdorffii × N. sanderae and transfer to honey by bees

AU - Helsper, J.P.F.G.

AU - Ruyter-Spira, C.P.

AU - Kwakman, P.H.S.

AU - Bleeker, W.K.

AU - Keizer, L.C.P.

AU - Bade, J.B.

AU - te Velde, A.A.

AU - Zaat, S.A.J.

AU - Verbeek, M.

AU - Creemers-Molenaar, J.

PY - 2011

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N2 - Honey has been used successfully in wound healing for thousands of years. The peptide hormone human epidermal growth factor (hEGF) is also known to have a beneficial effect in various wound healing processes via mechanisms that differ from those for honey. In this study, we show that hEGF can be incorporated into honey via nectar. Plants of Nicotiana langsdorffii × N. sanderae were transformed with the gene for hEGF, equipped with a nectary-targeted promoter and a signal sequence for secretion to nectar. These plants accumulated hEGF in the nectar. The maximum hEGF concentration recorded with ELISA in these plants is 2.5 ng·ml-1. There is a significant linear relationship (P <0.001) between hEGF concentration and induction of hEGF-receptor phosphorylation. Since the flower morphology of these plants did not allow production of honey from their nectar, we used feeding solutions, spiked with synthetic hEGF, to study transfer of this peptide into honey through bee activity. Transfer of hEGF from a feeding solution to honey by bees occurred with retention of the hEGF concentration and the capacity to induce hEGF-receptor phosphorylation. These observations indicate that plants can function as a production platform for honey containing biologically active peptides, which may enhance wound healing and other biological processes

AB - Honey has been used successfully in wound healing for thousands of years. The peptide hormone human epidermal growth factor (hEGF) is also known to have a beneficial effect in various wound healing processes via mechanisms that differ from those for honey. In this study, we show that hEGF can be incorporated into honey via nectar. Plants of Nicotiana langsdorffii × N. sanderae were transformed with the gene for hEGF, equipped with a nectary-targeted promoter and a signal sequence for secretion to nectar. These plants accumulated hEGF in the nectar. The maximum hEGF concentration recorded with ELISA in these plants is 2.5 ng·ml-1. There is a significant linear relationship (P <0.001) between hEGF concentration and induction of hEGF-receptor phosphorylation. Since the flower morphology of these plants did not allow production of honey from their nectar, we used feeding solutions, spiked with synthetic hEGF, to study transfer of this peptide into honey through bee activity. Transfer of hEGF from a feeding solution to honey by bees occurred with retention of the hEGF concentration and the capacity to induce hEGF-receptor phosphorylation. These observations indicate that plants can function as a production platform for honey containing biologically active peptides, which may enhance wound healing and other biological processes

KW - epidermal-growth-factor

KW - wound-healing process

KW - floral sources

KW - antioxidant capacity

KW - apis-mellifera

KW - in-vitro

KW - cells

KW - inhibition

KW - expression

KW - dressings

U2 - 10.1111/j.1438-8677.2010.00440.x

DO - 10.1111/j.1438-8677.2010.00440.x

M3 - Article

VL - 13

SP - 740

EP - 746

JO - Plant Biology

JF - Plant Biology

SN - 1435-8603

IS - 5

ER -