Accumulation of cynaropicrin in globe artichoke and localization ofenzymes involved in its biosynthesis

K. Eljounaidi, C. Comino, A. Moglia, K. Cankar, A. Genre, A. Hehn, F. Bourgaud, J. Beekwilder, S. Lanteri

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

Globe artichoke (Cynara cardunculus var. scolymus) belongs to the Asteraceae family, in which one ofthe most biologically significant class of secondary metabolites are sesquiterpene lactones (STLs). Inglobe artichoke the principal STL is the cynaropicrin, which contributes to approximately 80% of itscharacteristic bitter taste. Cynaropicrin content was assessed in globe artichoke tissues and was observedto accumulate in leaves of different developmental stages. In the receptacle, a progressive decrease wasobserved during inflorescence development, while the STL could not be detected in the inflorescencebracts. Almost undetectable amounts were found in the roots and inflorescence stems at the commercialstage. Cynaropicrin content was found to correlate with expression of genes encoding CcGAS, CcGAO andCcCOS, which are involved in the STL biosynthesis.A more detailed study of leaf material revealed that cynaropicrin predominantly accumulates in thetrichomes, and not in the apoplastic cavity fluids. Analysis of the promoter regions of CcGAO and CcCOSrevealed the presence of L1-box motifs, which confers trichome-specific expression in Arabidopsis, sug-gesting that cynaropicrin is not only stored but also synthesized in trichomes. A transient expression ofGFP fusion proteins was performed in Nicotiana benthamiana plants: the CcGAS fluorescence signal waslocated in the cytoplasm while the CcGAO and CcCOS localized to the endoplasmatic reticulum.
LanguageEnglish
Pages128-136
JournalPlant Science
Volume239
DOIs
Publication statusPublished - 2015

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Cynara scolymus
artichokes
sesquiterpenoid lactones
Sesquiterpenes
Lactones
biosynthesis
Trichomes
Cynara cardunculus subsp. cardunculus
Inflorescence
trichomes
Scolymus
Cynara
reticulum
Nicotiana benthamiana
Reticulum
Asteraceae
secondary metabolites
leaves
inflorescences
Arabidopsis

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Eljounaidi, K. ; Comino, C. ; Moglia, A. ; Cankar, K. ; Genre, A. ; Hehn, A. ; Bourgaud, F. ; Beekwilder, J. ; Lanteri, S. / Accumulation of cynaropicrin in globe artichoke and localization ofenzymes involved in its biosynthesis. In: Plant Science. 2015 ; Vol. 239. pp. 128-136.
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abstract = "Globe artichoke (Cynara cardunculus var. scolymus) belongs to the Asteraceae family, in which one ofthe most biologically significant class of secondary metabolites are sesquiterpene lactones (STLs). Inglobe artichoke the principal STL is the cynaropicrin, which contributes to approximately 80{\%} of itscharacteristic bitter taste. Cynaropicrin content was assessed in globe artichoke tissues and was observedto accumulate in leaves of different developmental stages. In the receptacle, a progressive decrease wasobserved during inflorescence development, while the STL could not be detected in the inflorescencebracts. Almost undetectable amounts were found in the roots and inflorescence stems at the commercialstage. Cynaropicrin content was found to correlate with expression of genes encoding CcGAS, CcGAO andCcCOS, which are involved in the STL biosynthesis.A more detailed study of leaf material revealed that cynaropicrin predominantly accumulates in thetrichomes, and not in the apoplastic cavity fluids. Analysis of the promoter regions of CcGAO and CcCOSrevealed the presence of L1-box motifs, which confers trichome-specific expression in Arabidopsis, sug-gesting that cynaropicrin is not only stored but also synthesized in trichomes. A transient expression ofGFP fusion proteins was performed in Nicotiana benthamiana plants: the CcGAS fluorescence signal waslocated in the cytoplasm while the CcGAO and CcCOS localized to the endoplasmatic reticulum.",
author = "K. Eljounaidi and C. Comino and A. Moglia and K. Cankar and A. Genre and A. Hehn and F. Bourgaud and J. Beekwilder and S. Lanteri",
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pages = "128--136",
journal = "Plant Science",
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Accumulation of cynaropicrin in globe artichoke and localization ofenzymes involved in its biosynthesis. / Eljounaidi, K.; Comino, C.; Moglia, A.; Cankar, K.; Genre, A.; Hehn, A.; Bourgaud, F.; Beekwilder, J.; Lanteri, S.

In: Plant Science, Vol. 239, 2015, p. 128-136.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Accumulation of cynaropicrin in globe artichoke and localization ofenzymes involved in its biosynthesis

AU - Eljounaidi, K.

AU - Comino, C.

AU - Moglia, A.

AU - Cankar, K.

AU - Genre, A.

AU - Hehn, A.

AU - Bourgaud, F.

AU - Beekwilder, J.

AU - Lanteri, S.

PY - 2015

Y1 - 2015

N2 - Globe artichoke (Cynara cardunculus var. scolymus) belongs to the Asteraceae family, in which one ofthe most biologically significant class of secondary metabolites are sesquiterpene lactones (STLs). Inglobe artichoke the principal STL is the cynaropicrin, which contributes to approximately 80% of itscharacteristic bitter taste. Cynaropicrin content was assessed in globe artichoke tissues and was observedto accumulate in leaves of different developmental stages. In the receptacle, a progressive decrease wasobserved during inflorescence development, while the STL could not be detected in the inflorescencebracts. Almost undetectable amounts were found in the roots and inflorescence stems at the commercialstage. Cynaropicrin content was found to correlate with expression of genes encoding CcGAS, CcGAO andCcCOS, which are involved in the STL biosynthesis.A more detailed study of leaf material revealed that cynaropicrin predominantly accumulates in thetrichomes, and not in the apoplastic cavity fluids. Analysis of the promoter regions of CcGAO and CcCOSrevealed the presence of L1-box motifs, which confers trichome-specific expression in Arabidopsis, sug-gesting that cynaropicrin is not only stored but also synthesized in trichomes. A transient expression ofGFP fusion proteins was performed in Nicotiana benthamiana plants: the CcGAS fluorescence signal waslocated in the cytoplasm while the CcGAO and CcCOS localized to the endoplasmatic reticulum.

AB - Globe artichoke (Cynara cardunculus var. scolymus) belongs to the Asteraceae family, in which one ofthe most biologically significant class of secondary metabolites are sesquiterpene lactones (STLs). Inglobe artichoke the principal STL is the cynaropicrin, which contributes to approximately 80% of itscharacteristic bitter taste. Cynaropicrin content was assessed in globe artichoke tissues and was observedto accumulate in leaves of different developmental stages. In the receptacle, a progressive decrease wasobserved during inflorescence development, while the STL could not be detected in the inflorescencebracts. Almost undetectable amounts were found in the roots and inflorescence stems at the commercialstage. Cynaropicrin content was found to correlate with expression of genes encoding CcGAS, CcGAO andCcCOS, which are involved in the STL biosynthesis.A more detailed study of leaf material revealed that cynaropicrin predominantly accumulates in thetrichomes, and not in the apoplastic cavity fluids. Analysis of the promoter regions of CcGAO and CcCOSrevealed the presence of L1-box motifs, which confers trichome-specific expression in Arabidopsis, sug-gesting that cynaropicrin is not only stored but also synthesized in trichomes. A transient expression ofGFP fusion proteins was performed in Nicotiana benthamiana plants: the CcGAS fluorescence signal waslocated in the cytoplasm while the CcGAO and CcCOS localized to the endoplasmatic reticulum.

U2 - 10.1016/j.plantsci.2015.07.020

DO - 10.1016/j.plantsci.2015.07.020

M3 - Article

VL - 239

SP - 128

EP - 136

JO - Plant Science

T2 - Plant Science

JF - Plant Science

SN - 0168-9452

ER -