Accounting for the decrease of photosystem photochemical efficiency with increasing irradiance to estimate quantum yield of leaf photosynthesis

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Abstract

Maximum quantum yield for leaf CO2 assimilation under limiting light conditions (UCO2LL) is commonly estimated as the slope of the linear regression of net photosynthetic rate against absorbed irradiance over a range of low-irradiance conditions. Methodological errors associated with this estimation have often been attributed either to light absorptance by non-photosynthetic pigments or to some data points being beyond the linear range of the irradiance response, both causing an underestimation of UCO2LL. We demonstrate here that a decrease in photosystem (PS) photochemical efficiency with increasing irradiance, even at very low levels, is another source of error that causes a systematic underestimation of UCO2LL. A model method accounting for this error was developed, and was used to estimate UCO2LL from simultaneous measurements of gas exchange and chlorophyll fluorescence on leaves using various combinations of species, CO2, O2, or leaf temperature levels. The conventional linear regression method under-estimated UCO2LL by ca. 10–15 %. Differences in the estimated UCO2LL among measurement conditions were generally accounted for by different levels of photorespiration as described by the Farquhar-von Caemmerer–Berry model. However, our data revealed that the temperature dependence of PSII photochemical efficiency under low light was an additional factor that should be accounted for in the model.
Original languageEnglish
Pages (from-to)323-335
JournalPhotosynthesis Research
Volume122
Issue number3
DOIs
Publication statusPublished - 2014

Keywords

  • temperature response functions
  • chlorophyll fluorescence
  • co2 uptake
  • electron-transport
  • c-3 photosynthesis
  • biochemical-model
  • limited photosynthesis
  • mesophyll conductance
  • vascular plants
  • o-2 evolution

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