Abundance, production and stabilization of microbial biomass under conventional and reduced tillage

K.J. van Groenigen, J. Bloem, E. Baath, P. Boeckx, J. Rousk, S. Bodé, P.D. Forristal, M.B. Jones

    Research output: Contribution to journalArticleAcademicpeer-review

    168 Citations (Scopus)

    Abstract

    Soil tillage practices affect the soil microbial community in various ways, with possible consequences for nitrogen (N) losses, plant growth and soil organic carbon (C) sequestration. As microbes affect soil organic matter (SOM) dynamics largely through their activity, their impact may not be deduced from biomass measurements alone. Moreover, residual microbial tissue is thought to facilitate SOM stabilization, and to provide a long term integrated measure of effects on the microorganisms. In this study, we therefore compared the effect of reduced (RT) and conventional tillage (CT) on the biomass, growth rate and residues of the major microbial decomposer groups fungi and bacteria. Soil samples were collected at two depths (0–5 cm and 5–20 cm) from plots in an Irish winter wheat field that were exposed to either conventional or shallow non-inversion tillage for 7 growing seasons. Total soil fungal and bacterial biomasses were estimated using epifluorescence microscopy. To separate between biomass of saprophytic fungi and arbuscular mycorrhizae, samples were analyzed for ergosterol and phospholipid fatty acid (PLFA) biomarkers. Growth rates of saprophytic fungi were determined by [14C]acetate-in-ergosterol incorporation, whereas bacterial growth rates were determined by the incorporation of 3H-leucine in bacterial proteins. Finally, soil contents of fungal and bacterial residues were estimated by quantifying microbial derived amino sugars. Reduced tillage increased the total biomass of both bacteria and fungi in the 0–5 cm soil layer to a similar extent. Both ergosterol and PLFA analyses indicated that RT increased biomass of saprophytic fungi in the 0–5 cm soil layer. In contrast, RT increased the biomass of arbuscular mycorrhizae as well as its contribution to the total fungal biomass across the whole plough layer. Growth rates of both saprotrophic fungi and bacteria on the other hand were not affected by soil tillage, possibly indicating a decreased turnover rate of soil microbial biomass under RT. Moreover, RT did not affect the proportion of microbial residues that were derived from fungi. In summary, our results suggest that RT can promote soil C storage without increasing the role of saprophytic fungi in SOM dynamics relative to that of bacteria.
    Original languageEnglish
    Pages (from-to)48-55
    JournalSoil Biology and Biochemistry
    Volume42
    Issue number1
    DOIs
    Publication statusPublished - 2010

    Keywords

    • arbuscular mycorrhizal fungi
    • particle-size fractions
    • amino sugar signature
    • leucine incorporation
    • carbon sequestration
    • enzyme-activities
    • c sequestration
    • organic-carbon
    • soil
    • bacterial

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